custom-built upright microscope Search Results


upright microscope  (Nikon)
99
Nikon upright microscope
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Upright Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/Objectives/pmc09980096-185-6-12
Average 99 stars, based on 1 article reviews
upright microscope - by Bioz Stars, 2026-09
99/100 stars
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orca r2 ccd camera  (Hamamatsu)
90
Hamamatsu orca r2 ccd camera
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Orca R2 Ccd Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/imagem+1k+camera/pmc04517217-390-12-22
Average 90 stars, based on 1 article reviews
orca r2 ccd camera - by Bioz Stars, 2026-09
90/100 stars
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fixed stage upright bx 51wi  (Olympus)
99
Olympus fixed stage upright bx 51wi
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Fixed Stage Upright Bx 51wi, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/BX61WI%2FBX51WI+Fixed+Stage+Microscope/pmc12866819-352-10-14
Average 99 stars, based on 1 article reviews
fixed stage upright bx 51wi - by Bioz Stars, 2026-09
99/100 stars
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olympus upright microscopes  (Olympus)
96
Olympus olympus upright microscopes
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Olympus Upright Microscopes, supplied by Olympus, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/CX23+Biological+Microscopes/pm40403705-926-31-31
Average 96 stars, based on 1 article reviews
olympus upright microscopes - by Bioz Stars, 2026-09
96/100 stars
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upright microscope  (Thorlabs)
86
Thorlabs upright microscope
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Upright Microscope, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/epifluorescence+microscope+upright/pm41275500-341-13-18
Average 86 stars, based on 1 article reviews
upright microscope - by Bioz Stars, 2026-09
86/100 stars
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fn1 upright microscope  (Nikon)
97
Nikon fn1 upright microscope
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Fn1 Upright Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/ECLIPSE+FN1/pmc04819566-82-13-16
Average 97 stars, based on 1 article reviews
fn1 upright microscope - by Bioz Stars, 2026-09
97/100 stars
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two photon microscope  (Scientifica)
86
Scientifica two photon microscope
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Two Photon Microscope, supplied by Scientifica, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/microscope+photon+two/pmc05653236-113-3-5
Average 86 stars, based on 1 article reviews
two photon microscope - by Bioz Stars, 2026-09
86/100 stars
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photon microscope  (Scientifica)
86
Scientifica photon microscope
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Photon Microscope, supplied by Scientifica, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/microscope+upright/pm41436654-385-8-17
Average 86 stars, based on 1 article reviews
photon microscope - by Bioz Stars, 2026-09
86/100 stars
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photometics 95b camera  (Photometics Inc)
90
Photometics Inc photometics 95b camera
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Photometics 95b Camera, supplied by Photometics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/photometics+95b+camera/pmc10891099-228-6-5
Average 90 stars, based on 1 article reviews
photometics 95b camera - by Bioz Stars, 2026-09
90/100 stars
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led source  (Thorlabs)
86
Thorlabs led source
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Led Source, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/led+light+source/us12551893-683-15-17
Average 86 stars, based on 1 article reviews
led source - by Bioz Stars, 2026-09
86/100 stars
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upright nikon c1 confocal laser scanning microscope  (Nikon)
99
Nikon upright nikon c1 confocal laser scanning microscope
A. representation of a head and body restrained mouse placed under <t>microscope</t> objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.
Upright Nikon C1 Confocal Laser Scanning Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+microscope/C2%2B/pm16163297-56-15-16
Average 99 stars, based on 1 article reviews
upright nikon c1 confocal laser scanning microscope - by Bioz Stars, 2026-09
99/100 stars
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Image Search Results


A. representation of a head and body restrained mouse placed under microscope objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.

Journal: bioRxiv

Article Title: Repeated passive visual experience modulates spontaneous and non-familiar stimulievoked neural activity

doi: 10.1101/2023.02.21.529278

Figure Lengend Snippet: A. representation of a head and body restrained mouse placed under microscope objective viewing visual stimuli. B. Experimental timeline (top). Each square indicates a day, and h1 and h2 are habituation days when mice were exposed to a gray screen. Spontaneous and visually evoked calcium transients were imaged in sessions S1 and S2. Visual stimuli consisted of full-field phase reversing grating stimuli of different orientations and natural images (NI; bottom). On the intervening days, mice experienced only one orientation of phase reversing grating stimulus. C. Representative standard deviation projection images from S1 (left) and S2 (right); scale bar: 50 μm. A neuron circled in blue is zoomed on the right; scale bar: 10 μm. D. Raster plot of calcium transients (dF/F0 indicated by the color bar) from identified neurons in the imaging field in C on S1 (left) and S2 (right). E. dF/F0 of calcium transients from the neuron circled in C on S1 (blue) and S2 (orange) during the entire imaging period encompassing gray screen, different grating, and natural images (represented as bars or NI on top) stimuli. Gray bars represent the stimulus duration. F. Trial-averaged dF/F0 (%) of the transients in E for each stimulus. GS and stim (3 seconds each) represent the gray screen and the indicated stimulus period, respectively. Arrow represents the start of the stimulus. Data presented as mean ± SEM. G. Population average of the mean trial-averaged dF/F0 for the indicated stimulus on S1 and S2. ** p <0.01; paired t test; n = 10 mice. Circles represent the average for each mouse and the lines connect the values from the same mouse in the two sessions. H. Scatterplot representing the relation between dF/F0 on S1 and S2. The circles represent neurons (561 neurons). The dotted line represents the approximate mean dF/F0 on S1 (4%) and values less and more than the mean are considered low and highly responsive neurons, respectively. The green line represents the identity line.

Article Snippet: Imaging was performed in a custom-built upright microscope with a 4X objective (Nikon).

Techniques: Microscopy, Standard Deviation, Imaging