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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: The human innate immune protein calprotectin induces iron starvation responses in Pseudomonas aeruginosa
doi: 10.1074/jbc.RA118.006819
Figure Lengend Snippet: P. aeruginosa metabolites affect iron depletion by CP. Tris/TSB was supplemented with 10 μm CP-Ser in the absence or presence of 5 μm PYO (A) or 2 μm pyoverdine (Pvd) (B) and incubated at 37 °C. At 4 and 8 h, CP-Ser was filtered out, and the metal content in the flow-through was analyzed by ICP-MS. The t = 0 h time point corresponds to filtered media before any CP, PYO, or pyoverdine supplementation and is thus the same for each condition (n = 3; **, p < 0.01).
Article Snippet: Samples were prepared in 15-ml Falcon tubes, and 2-ml samples were transferred to
Techniques: Incubation