Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: Comparative performance of four LIT modes, Luminex, and ELISA

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Luminex, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Incubation

Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: Schematic diagrams illustrating the methodologies used in Luminex® Assay ( a ) and ELISA ( b ). Figure was created with MedPeer (medpeer.cn). c Dose dependent median fluorescence intensity of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at different concentrations were compared between Standard LIT (black) and Luminex (red). d Dose dependent median fluorescence intensity and absorbance of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at different concentrations were compared between Standard LIT (black) and ELISA (red). Data presented as mean ± SD from three replicates. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Luminex, Enzyme-linked Immunosorbent Assay, Fluorescence, Standard Deviation

Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: a Dose-dependent median fluorescence intensity of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at various concentrations compared between Standard LIT assays with (red) and without (black) added serum. b Comparison of LODs for IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF with (pink) and without (blue) serum. c Spike recoveries for cytokines at high, medium, and low analyte concentrations. Linear regression plots comparing serum concentrations of IL-6 ( d ) and IL-8 ( e ) as determined by Luminex and LIT. Linear regression plots for IL-6 ( f ) and IL-8 ( g ) serum concentrations, determined by ELISA and LIT. Linear regression plots for IL-6 ( h ) and IL-8 ( i ) serum concentrations, determined by chemiluminescence and LIT. Data are presented as mean ± SD; n = 3 repeated tests. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Fluorescence, Comparison, Luminex, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: PloS one

Article Title: Assessing the role of STAT3 in DC differentiation and autologous DC immunotherapy in mouse models of GBM.

doi: 10.1371/journal.pone.0096318

Figure Lengend Snippet: Figure 2. The role of STAT3 signaling in the differentiation and expansion of DCs by Flt3L and GM-CSF. A. WT and STAT3 null bone marrow cells were cultured in the presence of hFlt3L (100 ng/ml) for 8 days then subsequently analyzed by flow cytometry for DC subtypes. Expression of CD45R was used to distinguish pDCs (CD11c+/CD45R+) from cDCs (CD11c+/CD45R2). The total number of CD11c+ DCs expanded from WT and STAT3 KO bone marrow was quantified from multiple independent bone marrow cultures (*, p,0.05; two-tail students t-test). B. Flow cytometry and quantification of GM-CSF-derived (40 ng/ml) BMDCs from WT and STAT3 null bone marrow cells. C. WT and STAT3 deficient mice were

Article Snippet: Recombinant mouse GM-CSF was procured from Abd Serotec (Raleigh, NC; cat# PMP82) and recombinant mouse Flt3L was purchased from Novus Biologicals, LLC (Littleton, CO; cat# NBCI-21336).

Techniques: Cell Culture, Flow Cytometry, Expressing, Derivative Assay

Journal: Cancer cell

Article Title: Oncogenic Kras-induced GM-CSF production promotes the development of pancreatic neoplasia

doi: 10.1016/j.ccr.2012.04.024

Figure Lengend Snippet: (A) Levels of GM-CSF mRNA (black bar) and protein (gray bar) in GFP-KrasG12D- PDEC were assessed by quantitative RT-PCR and ELISA respectively. Data is presented as an average fold induction over values from isogenic GFP-WT-PDEC. Error bars indicate SD, (n=3).

Article Snippet: Where indicated, mouse GM-CSF protein levels were determined by Mouse GM-CSF Quantikine ELISA Kit (mean MDD=1.8pg/ml; R&D Systems).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay