Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The C(t) normalization value (mean ± SD) of GLUT1 in bronchial brushing samples.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques:

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on NSCLC cell proliferation and colony formation. (A, B) NSCLC cell proliferation; (C, D) NSCLC cell colony formation. Adding siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; the graph in (C) and (D) shows the number of colony formation under different treatments; * , P <0.05 compared with negative control; NC, negative control; si-NC, scramble siRNA for negative control; Error bars, S.D.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on the cell cycle correlated proteins in NSCLC cells. We added siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; NC, negative control; si-NC, scramble siRNA for negative control.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on NSCLC cell migration and correlated proteins. (A, B) NSCLC cell migration; (C, D) the expressions of ROCK1, ROCK2 and RhoA. We added siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; the graph in (A) and (B) shows the distance of cell migration under different treatments; * , P <0.05 compared with negative control; NC, negative control; si-NC, scramble siRNA for negative control; Error bars, S.D.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Migration, Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on NSCLC cell invasion and correlated proteins. (A, B) NSCLC cell invasion; (C, D) the expressions of MMP2, proMMP2 and TIMP2. We added siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; the graph in (A) and (B) shows the number of invading cells under different treatments; * , P <0.05 compared with negative control; NC, negative control; si-NC, scramble siRNA for negative control; Error bars, S.D.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on the apoptosis in A549 and LK2 cells. We added siRNA-GLUT1 (siGLUT1) to A549 cell (A) and GLUT1 expression plasmid (GLUT1) to LK2 cell (B); NC, negative control; si-NC, scramble siRNA for negative control.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on integrin β1/Src/FAK signaling in NSCLC cells. We added siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; NC, negative control; si-NC, scramble siRNA for negative control.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Molecular medicine reports

Article Title: Combination of temozolomide and Taxol exerts a synergistic inhibitory effect on Taxol‑resistant glioma cells via inhibition of glucose metabolism.

doi: 10.3892/mmr.2015.4405

Figure Lengend Snippet: Figure 3. Taxol‑resistant cells exhibit upregulated glucose metabolism. (A) Generation of Taxol‑resistant cells from U251 glioma cells. Cells were treated with gradually increasing concentrations of Taxol in regular cell culture conditions for the selection of resistant cells. U251 Taxol‑resistant clones were pooled and analyzed following treatment with Taxol at the indicated concentrations for 48 h, and cell viability was measured. (B) Glucose uptake (Left) and lactate production (right) were measured in U251 Taxol‑sensitive and resistant cells. (C) Western blotting demonstrated that the expression levels of Glut1 and PDK1 were upregulated in Taxol‑resistant cells, as compared with the parental cells. β‑actin served as a loading control. Data are presented as the mean ± standard error of three indepen dent experiments. *P<0.05; **P<0.01. PDK1, phosphoinositide‑dependent kinase‑1; Glut1, glucose transporter 1; S, sensitive; R, resistant.

Article Snippet: A vector containing Myc-DDK-tagged wild-type Glut1 (cat. no. RC222696), was purchased from OriGene Technologies, Inc., Rockville, MD, USA).

Techniques: Cell Culture, Selection, Clone Assay, Western Blot, Expressing, Control

Journal: Molecular medicine reports

Article Title: Combination of temozolomide and Taxol exerts a synergistic inhibitory effect on Taxol‑resistant glioma cells via inhibition of glucose metabolism.

doi: 10.3892/mmr.2015.4405

Figure Lengend Snippet: Figure 2. TMZ‑resistant cells exhibit downregulated glucose metabolism. (A) Generation of TMZ‑resistant cells from U251 glioma cells. Cells were treated with gradually increasing concentrations of TMZ in regular cell culture conditions for the selection of resistant cells. U251 TMZ‑resistant clones were pooled and analyzed following treatment with TMZ at the indicated concentrations for 48 h, and cell viability was measured. (B) Glucose uptake (left) and lactate production (right) were measured in U251 TMZ‑sensitive and resistant cells. (C) Western blotting demonstrated that the expression of Glut1 and PDK1 were downregulated in TMZ‑resistant cells, as compared with the parental cells. β‑actin served as a loading control. Data are presented as the mean ± standard error of three independent experiments. *P<0.05; **P<0.01. TMZ, temozolomide; PDK1, phosphoinositide‑dependent kinase‑1; Glut1, glucose transporter 1; S, sensitive; R, resistant.

Article Snippet: A vector containing Myc-DDK-tagged wild-type Glut1 (cat. no. RC222696), was purchased from OriGene Technologies, Inc., Rockville, MD, USA).

Techniques: Cell Culture, Selection, Clone Assay, Western Blot, Expressing, Control

Journal: Molecular medicine reports

Article Title: Combination of temozolomide and Taxol exerts a synergistic inhibitory effect on Taxol‑resistant glioma cells via inhibition of glucose metabolism.

doi: 10.3892/mmr.2015.4405

Figure Lengend Snippet: Figure 5. Overexpression of Glut1 renders Taxol‑resistant cells unsusceptible to Taxol and TMZ combined treatment. (A) Treatment with a combination of Taxol and TMZ inhibited glucose uptake (left) and lactate production (right). Taxol‑resistant U251 glioma cells were treated with Taxol alone at 1 µM; TMZ alone at 5 µM, or Taxol + TMZ for 48 h, following which glucose uptake (left) and lactate production (right) were measured. (B) Western blotting demonstrated that the expression levels of Glut1 in U251 Taxol‑resistant cells transiently transfected with Glut1 were higher, as compared with cells transfected with a vector control (left). U251 Taxol‑resistant cells were transiently transfected with vector control or wild‑type Glut1 for 48 h, and were plated into 48‑well plates for the indicated treatments for 48 h, followed by measurements of cell viability. β‑actin served as a loading control. Data are presented as the mean ± standard error of three independent experiments. *P<0.05. TMZ, temozolomide; Ctrl, control; R, resistant; Glut1, glucose transporter 1; V, vector.

Article Snippet: A vector containing Myc-DDK-tagged wild-type Glut1 (cat. no. RC222696), was purchased from OriGene Technologies, Inc., Rockville, MD, USA).

Techniques: Over Expression, Western Blot, Expressing, Transfection, Plasmid Preparation, Control

Journal: International journal of molecular sciences

Article Title: MiR-22/GLUT1 Axis Induces Metabolic Reprogramming and Sorafenib Resistance in Hepatocellular Carcinoma.

doi: 10.3390/ijms26083808

Figure Lengend Snippet: Figure 4. GLUT1 is a target of miR-22 in HCC and regulates cell metabolism. (A) WB analysis of GLUT1 expression in the miR-22-silenced (MZIP-22) HepG2 cells and miR-22-overexpressing (pMXs-22) Huh-7 cells and related controls (shRNA). GAPDH was used as a housekeeping gene; WB analysis was performed in two independent experiments. GLUT1 molecular weight (MW) is from 45 to 60 kDa, and all the bands in this MW range have been considered for the analysis. (B) Dual-luciferase activity assay of wild-type (WT) and mutant (MUT) GLUT1-3′UTR vectors co-transfected with miR-22 in HepG2 and Huh-7 cells. NC: negative control precursor miRNA.

Article Snippet: GLUT1 (SLC2A1, NM_006516) 3′-UTR vector (SC215980) was purchased from Origene.

Techniques: Expressing, shRNA, Molecular Weight, Luciferase, Activity Assay, Mutagenesis, Transfection, Negative Control

Journal: International journal of molecular sciences

Article Title: MiR-22/GLUT1 Axis Induces Metabolic Reprogramming and Sorafenib Resistance in Hepatocellular Carcinoma.

doi: 10.3390/ijms26083808

Figure Lengend Snippet: Figure 5. miR-22 downregulation is associated with sorafenib resistance in HCC. (A) Growth curves of the miR-22-silenced (MZIP-22) HepG2 cells and miR-22-overexpressing (pMXs-22) Huh-7 cells and re- lated controls (shRNA) under sorafenib treatment. Rescue experiment in the miR-22-silenced (MZIP-22) HepG2 cells treated with the GLUT1-inhibitor BAY-876 or vehicle (DMSO) and sorafenib. The growth curves were normalized to T0. Mean ± SD values are reported. Two independent experiments were performed in quadruplicate. WB analysis of GLUT1 expression in the same setting. WB was performed in two independent experiments, and GAPDH was used as a housekeeping gene. (B,C) Box plot graph of the miR-22 (B) and GLUT1 (C) levels in responder (N = 8) and non-responder (N = 7) HCC nodules from sorafenib-treated rats. The Y-axis reports the 2−∆∆Ct values corresponding to the miR-22 or GLUT1 levels. Correlation graph between miR-22 (B) or GLUT1 (C) expression and tumor volume of sorafenib-treated DEN-HCC rats. The axes report the 2−∆∆Ct values corresponding to mRNA levels and tumor size (mm3) of HCC nodules transformed in a log2 form. Beta-actin or U6RNA were used as housekeeping genes. Real-Time PCR analysis was run in triplicate. (D) Correlation graphs between the

Article Snippet: GLUT1 (SLC2A1, NM_006516) 3′-UTR vector (SC215980) was purchased from Origene.

Techniques: shRNA, Expressing, Transformation Assay, Real-time Polymerase Chain Reaction

Journal: International journal of molecular sciences

Article Title: MiR-22/GLUT1 Axis Induces Metabolic Reprogramming and Sorafenib Resistance in Hepatocellular Carcinoma.

doi: 10.3390/ijms26083808

Figure Lengend Snippet: Figure 6. miR-22 circulating levels predict sorafenib resistance in human and rat HCCs. (A) Box plot graph of GLUT1 expression in early HCCs from the Bologna cohort divided based on serum miR-22 levels (high, N = 11; low, N = 10). The Y-axis reports the 2−∆∆Ct values. Mean ± SD values are displayed. GAPDH was used as a housekeeping gene. (B) Correlation graph between the tissue and serum miR-22 expression levels in early HCCs from the Bologna cohort. The axes report the 2−∆∆Ct values corresponding to the

Article Snippet: GLUT1 (SLC2A1, NM_006516) 3′-UTR vector (SC215980) was purchased from Origene.

Techniques: Expressing