Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The C(t) normalization value (mean ± SD) of GLUT1 in bronchial brushing samples.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques:

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on NSCLC cell proliferation and colony formation. (A, B) NSCLC cell proliferation; (C, D) NSCLC cell colony formation. Adding siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; the graph in (C) and (D) shows the number of colony formation under different treatments; * , P <0.05 compared with negative control; NC, negative control; si-NC, scramble siRNA for negative control; Error bars, S.D.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on the cell cycle correlated proteins in NSCLC cells. We added siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; NC, negative control; si-NC, scramble siRNA for negative control.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on NSCLC cell migration and correlated proteins. (A, B) NSCLC cell migration; (C, D) the expressions of ROCK1, ROCK2 and RhoA. We added siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; the graph in (A) and (B) shows the distance of cell migration under different treatments; * , P <0.05 compared with negative control; NC, negative control; si-NC, scramble siRNA for negative control; Error bars, S.D.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Migration, Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on NSCLC cell invasion and correlated proteins. (A, B) NSCLC cell invasion; (C, D) the expressions of MMP2, proMMP2 and TIMP2. We added siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; the graph in (A) and (B) shows the number of invading cells under different treatments; * , P <0.05 compared with negative control; NC, negative control; si-NC, scramble siRNA for negative control; Error bars, S.D.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on the apoptosis in A549 and LK2 cells. We added siRNA-GLUT1 (siGLUT1) to A549 cell (A) and GLUT1 expression plasmid (GLUT1) to LK2 cell (B); NC, negative control; si-NC, scramble siRNA for negative control.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Journal of Cancer

Article Title: Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling

doi: 10.7150/jca.30772

Figure Lengend Snippet: The effect of GLUT1 on integrin β1/Src/FAK signaling in NSCLC cells. We added siRNA-GLUT1 (siGLUT1) to A549 cell and GLUT1 expression plasmid (GLUT1) to LK2 cell; NC, negative control; si-NC, scramble siRNA for negative control.

Article Snippet: GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011).

Techniques: Expressing, Plasmid Preparation, Negative Control

Journal: Frontiers in Oncology

Article Title: Long Non-Coding RNA TMPO-AS1 Promotes GLUT1-Mediated Glycolysis and Paclitaxel Resistance in Endometrial Cancer Cells by Interacting With miR-140 and miR-143

doi: 10.3389/fonc.2022.912935

Figure Lengend Snippet: GLUT1 Expression and Clinical Importance in EC. (A) The mRNA expression of GLUT1 (cancer versus normal tissues) in pan-cancers was analyzed with the Oncomine database. The graphic demonstrates the number of datasets that meet our threshold for each cancer type. Red: up-regulation; blue: down-regulation. (B) The heatmap was generated by the ImaGEO database using two EC data sets (GSE17025 and GSE56026). (C) Up- or down-regulated genes in EC tissues compared to normal tissues (ImaGEO). (D) The GEO dataset was analyzed for GLUT1 expression in stage I EC samples and normal endometrium samples. (E) The expression of GLUT1 protein was examined in EC tissue and adjacent normal tissues (Human Protein Atlas database; scale bar: 100 μm). (F) The probability of overall survival in EC patients expressing high or low GLUT1 levels was assessed using the KM plotter database.

Article Snippet: A human GLUT1 3′-untranslated region (3′-UTR) luciferase reporter vector was purchased from OriGene (Rockville, MD).

Techniques: Expressing, Generated

Journal: Frontiers in Oncology

Article Title: Long Non-Coding RNA TMPO-AS1 Promotes GLUT1-Mediated Glycolysis and Paclitaxel Resistance in Endometrial Cancer Cells by Interacting With miR-140 and miR-143

doi: 10.3389/fonc.2022.912935

Figure Lengend Snippet: Critical Roles of GLUT1 in Promoting EC Cell Phenotypes. (A) Western blotting analysis of GLUT1 protein expression in a normal endometrial cell line (EM) and human EC cell lines. (B) Western blotting analysis of GLUT1 expression in GLUT1-overexpressing HHUA cells and Ishikawa cells with GLUT1 silencing. (C–F) EC cell proliferation (C) , invasion (D) , glucose consumption (E) , and lactate production (F) assays following GLUT1 overexpression or knockdown. (G, H) GLUT1-overexpressing HHUA cells (G) and Ishikawa cells after knockdown of GLUT1 (H) were treated with different concentrations of paclitaxel, and cell viability was examined using the CCK-8 assay. Vec: vector. *** P < 0.001.

Article Snippet: A human GLUT1 3′-untranslated region (3′-UTR) luciferase reporter vector was purchased from OriGene (Rockville, MD).

Techniques: Western Blot, Expressing, Over Expression, CCK-8 Assay, Plasmid Preparation

Journal: Frontiers in Oncology

Article Title: Long Non-Coding RNA TMPO-AS1 Promotes GLUT1-Mediated Glycolysis and Paclitaxel Resistance in Endometrial Cancer Cells by Interacting With miR-140 and miR-143

doi: 10.3389/fonc.2022.912935

Figure Lengend Snippet: KEGG Pathway and Wiki Pathway Enrichment Analysis of GLUT1 -Associated Genes in EC. (A) Genes that were found to be highly co-expressed with GLUT1 in the TCGA EC dataset from the LinkedOmics database were chosen. (B, C) Gene set enrichment analysis for positively correlated genes of GLUT1 in TCGA EC tissues. Enrichment analysis for KEGG pathways (B) and Wikipathway cancer (C) was performed using the LinkedOmics database.

Article Snippet: A human GLUT1 3′-untranslated region (3′-UTR) luciferase reporter vector was purchased from OriGene (Rockville, MD).

Techniques:

Journal: Frontiers in Oncology

Article Title: Long Non-Coding RNA TMPO-AS1 Promotes GLUT1-Mediated Glycolysis and Paclitaxel Resistance in Endometrial Cancer Cells by Interacting With miR-140 and miR-143

doi: 10.3389/fonc.2022.912935

Figure Lengend Snippet: GLUT1 is a Positive Upstream Regulator of MMP1, MMP14, and Cyclin D1. (A) The expression of GLUT1 was positively correlated with the expression of MMP1, MMP14 , and Cyclin D1 in EC tissues from the TCGA dataset. (B) qRT-PCR analysis of the indicated genes in EC cells after overexpression or knockdown of GLUT1. (C) The expression of MMP1, MMP14 , and Cyclin D1 in TCGA EC and normal tissues (ENCORI database). *** P < 0.001.

Article Snippet: A human GLUT1 3′-untranslated region (3′-UTR) luciferase reporter vector was purchased from OriGene (Rockville, MD).

Techniques: Expressing, Quantitative RT-PCR, Over Expression

Journal: Frontiers in Oncology

Article Title: Long Non-Coding RNA TMPO-AS1 Promotes GLUT1-Mediated Glycolysis and Paclitaxel Resistance in Endometrial Cancer Cells by Interacting With miR-140 and miR-143

doi: 10.3389/fonc.2022.912935

Figure Lengend Snippet: GLUT1 is a Target Gene of MiR-140 and MiR-143. (A) Venn diagram showing the overlapping miRNAs predicted by three online databases. (B) Computational prediction of duplex formation between miR-140/miR-143 and the GLUT1 3′-UTR region. (C) The expression of miR-140/miR-143 in TCGA EC tissues and normal tissues (miR-TV database). (D) qRT-PCR analysis of miR-140/miR-143 in EC and EM cells. (E) GLUT1 protein expression was examined using western blotting assays in EC cells transfected as indicated. (F) Luciferase reporter assays in Ishikawa cells transfected with the wild-type (WT) or mutant (MUT) GLUT1 3′-UTR, as well as miR-140/miR-143 mimic or a control mimic. *** P < 0.001.

Article Snippet: A human GLUT1 3′-untranslated region (3′-UTR) luciferase reporter vector was purchased from OriGene (Rockville, MD).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection, Luciferase, Mutagenesis

Journal: Frontiers in Oncology

Article Title: Long Non-Coding RNA TMPO-AS1 Promotes GLUT1-Mediated Glycolysis and Paclitaxel Resistance in Endometrial Cancer Cells by Interacting With miR-140 and miR-143

doi: 10.3389/fonc.2022.912935

Figure Lengend Snippet: TMPO-AS1 Induces GLUT1 Expression by Repressing MiR-140 and MiR-143 Levels. (A) GLUT1 protein expression was measured in Ishikawa and HHUA cells transfected with (or without) TMPO-AS1 siRNA, along with (or without) miR-140/miR-143 inhibitor. (B) Examination of MMP1 , MMP14 , and Cyclin D1 expression in Ishikawa cells transfected as indicated. (C) Correlation analysis between TMPO-AS1 and GLUT1 or between TMPO-AS1 and miR-140/miR-143. *** P < 0.001.

Article Snippet: A human GLUT1 3′-untranslated region (3′-UTR) luciferase reporter vector was purchased from OriGene (Rockville, MD).

Techniques: Expressing, Transfection

Journal: Frontiers in Oncology

Article Title: Long Non-Coding RNA TMPO-AS1 Promotes GLUT1-Mediated Glycolysis and Paclitaxel Resistance in Endometrial Cancer Cells by Interacting With miR-140 and miR-143

doi: 10.3389/fonc.2022.912935

Figure Lengend Snippet: Graphical Abstract showing that the LncRNA TMPO-AS1 Regulates Glycolysis and Paclitaxel Resistance in EC Cells by Affecting the MiR-140/MiR-143-GLUT1 Pathway.

Article Snippet: A human GLUT1 3′-untranslated region (3′-UTR) luciferase reporter vector was purchased from OriGene (Rockville, MD).

Techniques: