Journal: Cell reports

Article Title: Nuclear transport surveillance of p53 by nuclear pores in glioblastoma.

doi: 10.1016/j.celrep.2023.112882

Figure Lengend Snippet: Figure 6. PIM kinase mediates p53 degradation by preventing the proteasome pathway (A) Immunofluorescence of p53 in U87 cells after PIM447 treatment (10 mM) for 48 h showed an accumulation of p53 inside the nucleus. Scale bar: 10 mm. (B) Western blot analysis of U87 and A172 cells after PIM447 treatment (10 mM) for 48 h showed p53, p21, and MDM2 upregulation. (C) Growth assay by crystal violet staining in GBM cells after PIM447 treatment (10 mM) for 72 h incubation showed growth inhibition in the treated cells (n = 3, unpaired two-tailed Student’s t test, *p < 0.05).

Article Snippet: MG132 (Sigma, M8699) (50 mM, 4 h); KPT330 (Funakoshi, 18127) (1 nM, 4 h); Leptomycin B (Cell Signaling Technology, 9676) (10 nM, 3 h); PIM447 (Selleck, S7985) (10 mM, 48 h (Immunofluorescence), 72 h (crystal violet growth assay)), AZD1208 (Selleck, S7104) (50mM, 24 h (western blotting), 72 h (crystal violet growth assay)), Nutlin-3 (Funakoshi (CEM), CS-0295) (8 mM, 24 h), Cycloheximide (30 ng/mL, 2 and 4 h); Cisplatin (Selleck, S1166) (5 mM, 24 h); Etoposide (Cell Signaling Technology, 2200) (50 mM, 24 h), Hydrogen Peroxide 30% (Nacalai, 18411-25) (500 mM, 6 h) were used in this study.

Techniques: Western Blot, Growth Assay, Staining, Incubation, Inhibition, Two Tailed Test