Journal: Frontiers in Microbiology

Article Title: Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum

doi: 10.3389/fmicb.2021.765872

Figure Lengend Snippet: Representative features of the interaction between P. myriotylum (Pm) and P. oligandrum (Po). Here hyphae are imaged in the region where the two colonies have recently made contact using either brightfield microscopy coupled with trypan blue staining (A–C) or cryo-scanning electron microscopy (D–F) . (A) P. myriotylum from self-confrontation, (B) P. oligandrum from self-confrontation with spiny oogonia visible, (C) coiling of P. oligandrum hypha around the hypha of P. myriotylum and apparent penetration by P. oligandrum of P. myriotylum hypha. The brightfield images of the cultures from where P. oligandrum and P. myriotylum confront each other were stained with trypan blue. (D) P. myriotylum from self-confrontation, (E) P. oligandrum from self-confrontation, (F) coiling of a P. oligandrum hypha around a hypha of P. myriotylum at two different magnifications. The cryo-SEM images were taken from cultures growing on polycarbonate membranes using the same experimental set-up as was used for the Dual-RNAseq (see for images of these cultures). The solid amber arrow indicates examples of hyphal coiling and the empty arrow indicates an example of penetration.

Article Snippet: A cryo-scanning electron microscope (cryo-SEM) (Quorum PP3010T system integrated onto a Hitachi SU8010 FE-SEM) was also used to visualize the interaction between P. oligandrum and P. myriotylum .

Techniques: Microscopy, Staining, Electron Microscopy, RNA sequencing