crossval Search Results


matlab script crossval  (MathWorks Inc)
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Figure 2. IFITM incorporation does not affect incorporation and cleavage of resistant HIV-1 AD8 Env. A panel of resistant AD8 Env pseudoviruses (Vector, SQV-treated (immature), IFITM1, and IFITM3-containing) was produced and analyzed. (A) Analysis of virus maturation by p24 im- munoblot. (B) Immunoblot analysis of Env incorporation and processing for a representative pseu- dovirus panel. (C) Western blot analysis of IFITM incorporation into pseudoviruses. (D) Effects of IFITMs on HIV-1 pseudovirus infectivity. TZM-bl cells were infected and the resulting luciferase signal (normalized to Vector) was measured after 48 h. Data are means and standard deviations of triplicate values from two independent panels of pseudoviruses. (E) The average efficiency of Env precursor (gp160) proteolytic processing for two independent preparations measured by calculating the gp41/gp160 band density ratio. (F) Env incorporation assessed by calculating the ratio of the total Env signal (gp41 + gp160) over the sum of the p24 and p55 bands, averaged across two pseudoviral preparations. (G,H) Immunofluorescence analysis of HIV-1 GFP-Vpr (G) and Env (H) incorporation into single virions using the <t>anti-gp120</t> 2G12 antibody and anti-human AF647-conjugated secondary antibodies. The statistical analysis was performed using Student’s t-test. Significance: n.s., p > 0.05; *, 0.05 > p > 0.01; ***, p < 0.001.
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linear discriminant analysis matlab crossval function  (MathWorks Inc)
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Figure 2. IFITM incorporation does not affect incorporation and cleavage of resistant HIV-1 AD8 Env. A panel of resistant AD8 Env pseudoviruses (Vector, SQV-treated (immature), IFITM1, and IFITM3-containing) was produced and analyzed. (A) Analysis of virus maturation by p24 im- munoblot. (B) Immunoblot analysis of Env incorporation and processing for a representative pseu- dovirus panel. (C) Western blot analysis of IFITM incorporation into pseudoviruses. (D) Effects of IFITMs on HIV-1 pseudovirus infectivity. TZM-bl cells were infected and the resulting luciferase signal (normalized to Vector) was measured after 48 h. Data are means and standard deviations of triplicate values from two independent panels of pseudoviruses. (E) The average efficiency of Env precursor (gp160) proteolytic processing for two independent preparations measured by calculating the gp41/gp160 band density ratio. (F) Env incorporation assessed by calculating the ratio of the total Env signal (gp41 + gp160) over the sum of the p24 and p55 bands, averaged across two pseudoviral preparations. (G,H) Immunofluorescence analysis of HIV-1 GFP-Vpr (G) and Env (H) incorporation into single virions using the <t>anti-gp120</t> 2G12 antibody and anti-human AF647-conjugated secondary antibodies. The statistical analysis was performed using Student’s t-test. Significance: n.s., p > 0.05; *, 0.05 > p > 0.01; ***, p < 0.001.
Linear Discriminant Analysis Matlab Crossval Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. IFITM incorporation does not affect incorporation and cleavage of resistant HIV-1 AD8 Env. A panel of resistant AD8 Env pseudoviruses (Vector, SQV-treated (immature), IFITM1, and IFITM3-containing) was produced and analyzed. (A) Analysis of virus maturation by p24 im- munoblot. (B) Immunoblot analysis of Env incorporation and processing for a representative pseu- dovirus panel. (C) Western blot analysis of IFITM incorporation into pseudoviruses. (D) Effects of IFITMs on HIV-1 pseudovirus infectivity. TZM-bl cells were infected and the resulting luciferase signal (normalized to Vector) was measured after 48 h. Data are means and standard deviations of triplicate values from two independent panels of pseudoviruses. (E) The average efficiency of Env precursor (gp160) proteolytic processing for two independent preparations measured by calculating the gp41/gp160 band density ratio. (F) Env incorporation assessed by calculating the ratio of the total Env signal (gp41 + gp160) over the sum of the p24 and p55 bands, averaged across two pseudoviral preparations. (G,H) Immunofluorescence analysis of HIV-1 GFP-Vpr (G) and Env (H) incorporation into single virions using the anti-gp120 2G12 antibody and anti-human AF647-conjugated secondary antibodies. The statistical analysis was performed using Student’s t-test. Significance: n.s., p > 0.05; *, 0.05 > p > 0.01; ***, p < 0.001.

Journal: Viruses

Article Title: IFITM1 and IFITM3 Proteins Inhibit the Infectivity of Progeny HIV-1 without Disrupting Envelope Glycoprotein Clusters.

doi: 10.3390/v15122390

Figure Lengend Snippet: Figure 2. IFITM incorporation does not affect incorporation and cleavage of resistant HIV-1 AD8 Env. A panel of resistant AD8 Env pseudoviruses (Vector, SQV-treated (immature), IFITM1, and IFITM3-containing) was produced and analyzed. (A) Analysis of virus maturation by p24 im- munoblot. (B) Immunoblot analysis of Env incorporation and processing for a representative pseu- dovirus panel. (C) Western blot analysis of IFITM incorporation into pseudoviruses. (D) Effects of IFITMs on HIV-1 pseudovirus infectivity. TZM-bl cells were infected and the resulting luciferase signal (normalized to Vector) was measured after 48 h. Data are means and standard deviations of triplicate values from two independent panels of pseudoviruses. (E) The average efficiency of Env precursor (gp160) proteolytic processing for two independent preparations measured by calculating the gp41/gp160 band density ratio. (F) Env incorporation assessed by calculating the ratio of the total Env signal (gp41 + gp160) over the sum of the p24 and p55 bands, averaged across two pseudoviral preparations. (G,H) Immunofluorescence analysis of HIV-1 GFP-Vpr (G) and Env (H) incorporation into single virions using the anti-gp120 2G12 antibody and anti-human AF647-conjugated secondary antibodies. The statistical analysis was performed using Student’s t-test. Significance: n.s., p > 0.05; *, 0.05 > p > 0.01; ***, p < 0.001.

Article Snippet: Viruses 2023, 15, 2390 3 of 17 ab109429), rabbit anti-IFITM1 (Sigma, St. Louis, MO, USA, cat. no. HPA004810), goat anti-HIV gp120 (Fitzgerald, Crossville, TN, USA, cat. no. 20HG-81), mouse anti-GAPDH (Proteintech, Rosemont, IL, USA, cat. no. 60004), goat anti-human IgG HRP (ThermoFisher Scientific, Waltham, MA, USA, cat. no. 31412), mouse anti-rabbit IgG HRP (Millipore, Temecula, CA, USA, cat. no. AP188P), rabbit anti-mouse IgG HRP (EMD Millipore, St. Louis, MO, USA, cat. No. AP160P), donkey anti-goat IgG HRP (Santa Cruz Biotechnology, Dallas, TX, USA, cat. no. sc-2020), and goat anti-human IgG (H+L) conjugated with AlexaFluor 647 (AF647, ThermoFisher, cat. No. A21445).

Techniques: Plasmid Preparation, Produced, Virus, Western Blot, Infection, Luciferase