crispr design Search Results


pcr genotyping  (Transnetyx)
99
Transnetyx pcr genotyping
Pcr Genotyping, supplied by Transnetyx, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr genotyping/product/Transnetyx
Average 99 stars, based on 1 article reviews
pcr genotyping - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
crispr design platform  (Broad Institute Inc)
90
Broad Institute Inc crispr design platform
Crispr Design Platform, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr design platform/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
crispr design platform - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
crispr design tool  (ATUM Bio)
90
ATUM Bio crispr design tool
Crispr Design Tool, supplied by ATUM Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr design tool/product/ATUM Bio
Average 90 stars, based on 1 article reviews
crispr design tool - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
optimized crispr design application  (Shanghai GenePharma)
90
Shanghai GenePharma optimized crispr design application
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Optimized Crispr Design Application, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/optimized crispr design application/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
optimized crispr design application - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
crispr-cas9 in frame with paprikarfp  (ATUM Bio)
90
ATUM Bio crispr-cas9 in frame with paprikarfp
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Crispr Cas9 In Frame With Paprikarfp, supplied by ATUM Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr-cas9 in frame with paprikarfp/product/ATUM Bio
Average 90 stars, based on 1 article reviews
crispr-cas9 in frame with paprikarfp - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
crispr sgrna design tool  (GenScript corporation)
90
GenScript corporation crispr sgrna design tool
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Crispr Sgrna Design Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr sgrna design tool/product/GenScript corporation
Average 90 stars, based on 1 article reviews
crispr sgrna design tool - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
crispr primer designer software  (GenScript corporation)
90
GenScript corporation crispr primer designer software
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Crispr Primer Designer Software, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr primer designer software/product/GenScript corporation
Average 90 stars, based on 1 article reviews
crispr primer designer software - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
gencrispr grna design tool  (GenScript corporation)
90
GenScript corporation gencrispr grna design tool
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Gencrispr Grna Design Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gencrispr grna design tool/product/GenScript corporation
Average 90 stars, based on 1 article reviews
gencrispr grna design tool - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
sanger crispr webtool  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc sanger crispr webtool
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Sanger Crispr Webtool, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sanger crispr webtool/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
sanger crispr webtool - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
crispr tool  (GenScript corporation)
90
GenScript corporation crispr tool
Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the <t>CRISPR/Cas9</t> and LoxP systems. <t>Two</t> <t>sgRNAs</t> were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Crispr Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr tool/product/GenScript corporation
Average 90 stars, based on 1 article reviews
crispr tool - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
crispr design assistance  (Vir Biotechnology)
90
Vir Biotechnology crispr design assistance
Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the <t>CRISPR/Cas9</t> and LoxP systems. <t>Two</t> <t>sgRNAs</t> were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Crispr Design Assistance, supplied by Vir Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr design assistance/product/Vir Biotechnology
Average 90 stars, based on 1 article reviews
crispr design assistance - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
crispr-p online primer design software  (PrimerDesign Inc)
90
PrimerDesign Inc crispr-p online primer design software
Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the <t>CRISPR/Cas9</t> and LoxP systems. <t>Two</t> <t>sgRNAs</t> were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Crispr P Online Primer Design Software, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr-p online primer design software/product/PrimerDesign Inc
Average 90 stars, based on 1 article reviews
crispr-p online primer design software - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


The stable SH-SY5Y cells, expressing AMPKα1 shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing CRISPR/Cas-9-AMPKα1 (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.

Journal: Oncotarget

Article Title: AMPK activation by Tanshinone IIA protects neuronal cells from oxygen-glucose deprivation

doi: 10.18632/oncotarget.23391

Figure Lengend Snippet: The stable SH-SY5Y cells, expressing AMPKα1 shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing CRISPR/Cas-9-AMPKα1 (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.

Article Snippet: The small guide RNA (sgRNA) targeting human AMPKα1 was based on the Optimized CRISPR Design application ( http://crispr.mit.edu/ ), which was provided by Genepharm (Shanghai, China).

Techniques: Expressing, shRNA, Control, CRISPR, Quantitative RT-PCR, MTT Assay, Lactate Dehydrogenase Assay

Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the CRISPR/Cas9 and LoxP systems. Two sgRNAs were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.

Journal: Viruses

Article Title: Genomic Characterization and gE/gI-Deleted Strain Construction of Novel PRV Variants Isolated in Central China

doi: 10.3390/v15061237

Figure Lengend Snippet: Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the CRISPR/Cas9 and LoxP systems. Two sgRNAs were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.

Article Snippet: sgRNAs targeting the gE and gI genes were designed using an online CRISPR tool ( https://www.genscript.com/gRNA-design-tool.html , accessed on 15 May 2021).

Techniques: Mutagenesis, Virus, CRISPR