Journal: PLoS ONE

Article Title: Melanocyte progenitor cells reside in human subcutaneous adipose tissue

doi: 10.1371/journal.pone.0256622

Figure Lengend Snippet: The expression levels of HMB45, MITF, MelEM, Mel2, MelanA, MATP, and tyrosinase (TYR) in undifferentiated and differentiated ADSCs, as well as NHEMs, were examined by immunofluorescence microscopy. All melanocytic markers except for TYR were detected.

Article Snippet: In addition, undifferentiated and differentiated ADSCs were fixed for 30 min in 4% paraformaldehyde at room temperature and blocked with PBS containing 0.1% Triton (Wako) and 10% goat serum (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for 1 h at room temperature followed by a 1 h incubation at 37°C with primary antibodies against microphthalmia-associated transcription factor (MITF, 1:250, Abcam Biotechnology), Melan A (1:1000, Abcam Biotechnology), Mel2 (1:500, Abcam Biotechnology), MelEM (1:1; Developmental Studies Hybridoma Bank), MATP (1:400; Abcam Biotechnology), and Tyrosinase (TYR, 1:250, Abcam Biotechnology).

Techniques: Expressing, Immunofluorescence, Microscopy