Journal: Toxics

Article Title: Effects of Short-Term Exposure to Polystyrene Nanoplastics on the Nervous System: Calcium Homeostasis, BDNF and Synaptic Plasticity

doi: 10.3390/toxics14020178

Figure Lengend Snippet: Role of BDNF in the mechanism of synaptic plasticity impairment. All indicators were detected in neurons from the control and PS-NPs intervention groups. ( A ) Western blot analysis of CREB, BDNF, Cav3.1, and CaMKIIα in neurons, with GAPDH as the loading control. ( B ) Protein expression levels of CREB in the neurons. n = 3. ( C ) Protein expression levels of BDNF in the neurons. n = 3. ( D ) Calcium ion content in the neurons. n = 6. ( E ) Protein expression levels of the calcium channel Cav3.1 in neurons. ( n = 3). ( F ) Protein expression levels of CaMKIIα in neurons. n = 3. (○) represents the individual samples within each group. Quantitative results were normalized, and the data in the bar charts are presented as mean ± SD. ** p < 0.01 or *** p < 0.001, **** p < 0.0001.

Article Snippet: The membranes were blocked for 1 h at room temperature with 5% skim milk (Beyotime, Shanghai, China, P0216-1500 g) and incubated overnight at 4 °C with specific primary antibodies, including Cav3.1 (1:2000, NBP2-59322, Novus, Lone Tree, CO, USA), CaMKIIα (1:2000, 3357S, CST, Danvers, MA, USA), CREB (1:2000, 12208-1-AP, Proteintech, Rosemont, IL, USA), BDNF (1:1000, ab226843, abcam, Cambridge, UK), PSD95 (1:1000, 2507S, CST), SYN (1:2000, 17785-1-AP, Proteintech), ProBDNF (1:1000, 28205-1-AP, Proteintech), and GAPDH (1:2000, UM4002, YouKang, Tianjin, China).

Techniques: Control, Western Blot, Expressing

Journal: Chinese Medicine

Article Title: Electroacupuncture alleviates cognitive impairments in APP/PS1 mice via gastric vagal afferent-mediated activation of the nucleus tractus solitarius‒locus coeruleus noradrenergic circuit

doi: 10.1186/s13020-025-01230-x

Figure Lengend Snippet: EA suppresses neuroinflammation in the hippocampus of APP/PS1 mice by activating the β2-AR/PKA/CREB pathway. A Representative Western blots showing the levels of ADRB, PKA, p-PKA, CREB, p-CREB, IL-6, IL-1β, and TNF-α in the hippocampi of the mice in each group. B – I Data represent the mean ± SD (n = 4). * P < 0.05 and ** P < 0.01 by one-way ANOVA

Article Snippet: p-CREB , / , 1:300 , ABclonal , Rabbit , AP0903.

Techniques: Western Blot

Journal: bioRxiv

Article Title: β2-Adrenergic Biased Agonist Nebivolol Inhibits the Development of Th17 and the Response of Memory Th17 Cells in an NF-κB-Dependent Manner

doi: 10.1101/2024.09.08.611829

Figure Lengend Snippet: Blasted memory Th cells activated with antiCD3/antiCD28/antiCD2 for 15 minutes in conditions of non-activated cells, activated cells, activated cells plus nebivolol, terbutaline A) Representative western blot data of equal amounts of protein from the cell lysates was shown for p-CREB and α-tubulin as a loading control. B) Band intensity was quantified and shown corrected to the loading control as a ratio, with data pooled from three independent biological experiments. C) Memory Th cells were activated for five days, with nebivolol plus or minus H89. A representative IL-17A ELISA data for the effect of PKA in nebivolol’s signaling pathway by using H89 as a PKA inhibitor. ANOVA was followed by Tukey’s multiple comparisons tests.

Article Snippet: Antibodies were against human Phospho-ser529 NF-κB p65 (1/500, clone A21012B Biolegend), NF-κB p65 Antibody (1/1000, clone 14G10A21, Biolegend), phospho-Ser133 CREB1 (1/700, rabbit polyclonal, Cusabio, Cedarlane), phospho-Ser412-β-arrestin1 (1/1000, clone mAb #2416 Cell Signaling Tech., Danvers, USA), β2AR (1/500, clone 4A6C9, Novusbio, Cedarlane), GAPDH (1/15000, 10B4E3, MA000071M1m, Cusabio, Cedarlane) and α□tubulin (1/1000, Santa Cruz Biotechnology Inc., USA).

Techniques: Western Blot, Control, Enzyme-linked Immunosorbent Assay