Journal: PLoS ONE

Article Title: Sodium-Glucose Cotransporter 2 Inhibitor and a Low Carbohydrate Diet Affect Gluconeogenesis and Glycogen Content Differently in the Kidney and the Liver of Non-Diabetic Mice

doi: 10.1371/journal.pone.0157672

Figure Lengend Snippet: Taqman (R) gene expression assays annotation.

Article Snippet: Mm00501607_m1 , Creb1 , cAMP responsive element binding protein 1 , NM_009952.2.

Techniques: Gene Expression, Binding Assay

Journal: PLoS ONE

Article Title: Sodium-Glucose Cotransporter 2 Inhibitor and a Low Carbohydrate Diet Affect Gluconeogenesis and Glycogen Content Differently in the Kidney and the Liver of Non-Diabetic Mice

doi: 10.1371/journal.pone.0157672

Figure Lengend Snippet: Foxo1 and Creb1 mRNA expression in the kidney (a, b) and liver (c, d) were determined by quantitative RT-PCR. The means ± SEM of mRNA related to NC group are presented. n = 6–10. *p < 0.05, **p < 0.01 vs NC. White bars, NC; hatched white bars, LC; hatched grey bars, NC+Ipra; black bars LC+Ipra.

Article Snippet: Mm00501607_m1 , Creb1 , cAMP responsive element binding protein 1 , NM_009952.2.

Techniques: Expressing, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Dibutyryl cyclic AMP downregulates tenascin-C in neurons and astrocytes and reduces AAV-mediated gene expression in DRG neurons

doi: 10.1101/2025.05.13.653846

Figure Lengend Snippet: ( A ) Representative snapshots from Imaris software of DRGs visualized using the Zeiss Lightsheet Z.1 following ECi clearing. Scale bar: 400 µm. ( B ) Representative confocal images of DRG sections showing varying signal intensities in the presence and absence of db-cAMP. For intensity measurements, all stained sections were imaged using the same laser power, gain, and airy units. Scale bar: 400 µm. ( C ) Quantification of (A). Bar graphs display the total number of GFP-positive or V5-positive cells in the DRG. Data are presented as mean ± SEM, analysed by unpaired t-test, ns > 0.5, *p < 0.05, n = 6 DRGs/group. ( D ) Quantification of (B). Bar graphs show the total GFP-positive or V5-positive signal per DRG. Data are presented as mean ± SEM, analysed by unpaired t-test, **p < 0.01, ****p < 0.0001, n = 8 DRGs/group, with three sections evaluated per DRG to obtain the final value. ( E, F ) Immunoblotting results for GFP, PKA C-α, β-actin, and vinculin in DRG samples from animals injected with GFP only and those co-injected with db-cAMP 13 weeks post-injection. Values were normalised to vinculin. Two housekeeping proteins were utilised to enhance the reliability and accuracy of the results. Results are presented as mean density values ± SEM; n = 3 DRGs, with western blots repeated four times in separate experiments; ns > 0.5, *p < 0.05, **p < 0.01, analysed by unpaired t-test. ( G ) Heat maps illustrate Log 2 fold changes (as Log 2 (2^-ΔΔCt)) for the expression of Creb1 and Itg α 9 , normalised to the uninjured, uninjected control. Log 2 (2^-ΔΔCt) values were determined through qRT-PCR analysis. Results are presented as a heat map.

Article Snippet: For qPCR, TaqMan® gene expression assays (Life Technologies by Thermo Fisher Scientific, Waltham, MA, USA) were used for CREB1 (Rn00578828_g1), Itga9 (Rn01746751_m1) and GAPDH (Rn01775763_g1), all purchased from Applied Biosystems and used according to the manufacturer’s recommendations.

Techniques: Software, Staining, Western Blot, Injection, Expressing, Control, Quantitative RT-PCR

Journal: The FASEB Journal

Article Title: Prolonged activation of cAMP signaling leads to endothelial barrier disruption via transcriptional repression of RRAS

doi: 10.1096/fj.201700818RRR

Figure Lengend Snippet: RNA sequence analysis of human lung microvascular ECs

Article Snippet: Expression vectors pCMV-N-Flag and pCMV-N-Flag-CREB1 encoding a full-length cDNA for human CREB1 flanked with an N-terminal Flag tag were purchased from Sino Biological (Beijing, China).

Techniques: Sequencing

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Macrophage migration inhibitory factor mediates joint capsule fibrosis via facilitating phospholipid metabolite PGE2 production in fibroblasts

doi: 10.1007/s00018-025-05800-y

Figure Lengend Snippet: Effects of CREB on the MIF-induced COX2 expression and PGE2 production. A , Western blot analysis of p-CREB following joint capsule fibroblasts treated with 2 µg/mL MIF for 0, 15, 30, 60, and 120 min. B , Quantitative results of A. C , Western blot analysis of p-CREB in joint capsule fibroblasts following siRNA2 knockdown of CD74 for 48 h and 2 µg/mL MIF stimulation for 15 min. D , Quantitative results of C. E , Evaluation of CREB knockdown efficiency using qRT-PCR after transfection for 48 h and siRNA1 was selected for subsequent experiments. F–H , Following siRNA1 knockdown of CREB for 48 h and 2 μg/ml MIF stimulation for 24 h, COX2 expression in joint capsule fibroblasts was assessed by qRT-PCR ( F ) and Western blot ( G ). Quantitative results of G as shown in ( H ). I and J , PGE2 production in supernatant ( I ) and lysate ( J ) of joint capsule fibroblasts were tested by ELISA accordingly. Each experiment was conducted three times. Error bars denote standard deviation. * P < 0.05 compared with Control group or 0 min group

Article Snippet: CREB gene silencing (RiboBio, Guangzhou, China) was similarly performed using different siRNA sequences (siRNA1, siRNA2, siRNA3) following the same procedures.

Techniques: Expressing, Western Blot, Knockdown, Quantitative RT-PCR, Transfection, Enzyme-linked Immunosorbent Assay, Standard Deviation, Control