Journal: International journal of molecular sciences

Article Title: Isoliquiritigenin Ameliorates High-Fat Diet-Induced Obesity in Mice by Activating Brown Adipose Tissue.

doi: 10.3390/ijms26041616

Figure Lengend Snippet: Figure 3. ISL treatment enhanced the expression of mitochondrial biogenesis and thermogenesis- related genes and proteins. (A) The mRNA levels of UCP1, UCP2, PPARGC1a, and PRDM16 in the BAT (n = 3). (B) Representative UCP1 staining of BAT (n = 3). (C,D) The protein levels of PPARGC1a, UCP1, and CKB in BAT (n = 3). * p < 0.05, ** p < 0.01.

Article Snippet: The following primary antibodies were used: C/EBPa (Proteintech, 18311-1-AP, 1:2000), FASN (Proteintech, 10624-2-AP, 1:5000), GLUT4 (Santa Cruz Biotechnology, Dallas, TX, USA, sc-53566, 1:2000), PPARG (Proteintech, 16643-1-AP, 1:2000), FABP4 (Proteintech, 12802- 1-AP, 1:6000), ATGL (Proteintech, 55190-1-AP, 1:2000), PPARGC1a (Proteintech, 66369-1-Ig, 1:6000), CKB (Proteintech, 15137-1-AP, 1:5000), UCP1 (Proteintech, 23673-1-AP, 1:2000), and GAPDH (Proteintech, 10494-1-AP, 1:5000).

Techniques: Expressing, Staining

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Golden bifid treatment regulates gut microbiota and serum metabolites to improve myocardial dysfunction in cecal ligation and puncture-induced sepsis mice.

doi: 10.1016/j.bbadis.2024.167049

Figure Lengend Snippet: Fig. 1. Effects of probiotics on body weight and cardiac function in myocardial injury model in sepsis mice Three groups were set: control (sham surgery), cecal ligation and puncture (CLP)-induced sepsis group, and CLP-induced sepsis plus probiotics (golden bifid) treatment group. Mice were randomly allocated into three groups and treated accordingly. (A) Mice weight was monitored on days 0, 2, and 4 of modeling. (B) The surviving hours of mice were analyzed using survival curves. (C) Cardiac functions were evaluated using Echocardiography. (D) Serum CK-MB release was determined using ELISA. (E) Histopathological alterations were evaluated using H&E staining. (F) Myocardial apoptosis was examined using TUNEL staining. (G-H) Cardiomyocyte size change was determined by WGA staining. (I) The protein levels of Bax, Bcl-2, and cleaved-caspase 3 using Immunoblotting. ** p < 0.01, vs. control group; ## p < 0.01, vs. CLP group.

Article Snippet: The CK-MB concentration within the serum was measured using the Mouse CK-MB enzyme-linked immunosorbent assay (ELISA) kit (CSBE14404m, CUSABIO, China).

Techniques: Probiotics, Control, Ligation, Enzyme-linked Immunosorbent Assay, Staining, TUNEL Assay, Western Blot

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Golden bifid treatment regulates gut microbiota and serum metabolites to improve myocardial dysfunction in cecal ligation and puncture-induced sepsis mice.

doi: 10.1016/j.bbadis.2024.167049

Figure Lengend Snippet: Fig. 2. Effects of probiotics on inflammatory and oxidative stress-related indicators in myocardial injury model in sepsis mice (A) The levels of TNF-α, IL-1β, and IL-6 in myocardial tissues were examined using ELISA. (B) The levels of TNF-α, IL-1β, and IL-6 in mice serum were examined using ELISA. (C) The protein levels of SOD were examined using Immunoblotting. (D) The activity of SOD was determined using ELISA. (E) The levels of oxidative damage marker 8-OHdG were determined using ELISA. ** p < 0.01, vs. control group; # p < 0.05, ## p < 0.01, vs. CLP group.

Article Snippet: The CK-MB concentration within the serum was measured using the Mouse CK-MB enzyme-linked immunosorbent assay (ELISA) kit (CSBE14404m, CUSABIO, China).

Techniques: Probiotics, Enzyme-linked Immunosorbent Assay, Western Blot, Activity Assay, Marker, Control

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Golden bifid treatment regulates gut microbiota and serum metabolites to improve myocardial dysfunction in cecal ligation and puncture-induced sepsis mice.

doi: 10.1016/j.bbadis.2024.167049

Figure Lengend Snippet: Fig. 3. Effects of probiotics on intestinal mucosal permeability and barrier function in myocardial injury model in sepsis mice (A) The intestinal mucous layer thickness was determined using Alcian blue staining. (B) The levels of LPS in serum were determined using ELISA. (C) The levels and distribution of tight junction protein ZO-1 and occludin were detected by Immunofluorescence staining (IF). ** p < 0.01, vs. control group; # p < 0.05, vs. CLP group.

Article Snippet: The CK-MB concentration within the serum was measured using the Mouse CK-MB enzyme-linked immunosorbent assay (ELISA) kit (CSBE14404m, CUSABIO, China).

Techniques: Probiotics, Permeability, Staining, Enzyme-linked Immunosorbent Assay, Immunofluorescence, IF-P, Control