Journal: British Journal of Cancer

Article Title: Inhibition of the AURKA/YAP1 axis is a promising therapeutic option for overcoming cetuximab resistance in colorectal cancer stem cells

doi: 10.1038/s41416-024-02649-z

Figure Lengend Snippet: a Plot representing the distribution of YAP1 expression in CRC cell lines sensitive to cetuximab compared to the resistant ones ns =non-significant * p < 0.05, two-tailed t-Student’s test. b Plot depicting the distribution of YAP1 activity score in cetuximab-sensitive CRC cell lines compared to the resistant ones. c Correlation analysis between AURKA expression and YAP1 activity score. d Correlation analysis between AURKA and YAP1 expression levels. e Western blot illustrating the basal levels of total amount and phosphorylated YAP1 (Ser397) in CRC cell lines HCA46, SW48 and C10. Tubulin was used as a loading control. Results are plotted as the average ± SD of all the biological replicates and were normalised to the HCA46 cell line ( n = 3). ns =non-significant, ** p < 0.01, *** p < 0.001, one-way ANOVA. f Gene expression levels of CTGF and CYR61 in CRC cell lines HCA46, SW48 and C10 ( n = 3). ns =non-significant, ** p < 0.01, *** p < 0.001, one-way ANOVA.

Article Snippet: Human CRC cell line HCA46 was obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Expressing, Two Tailed Test, Activity Assay, Western Blot, Control, Gene Expression

Journal: British Journal of Cancer

Article Title: Inhibition of the AURKA/YAP1 axis is a promising therapeutic option for overcoming cetuximab resistance in colorectal cancer stem cells

doi: 10.1038/s41416-024-02649-z

Figure Lengend Snippet: a Western blot illustrating c-MET levels in the HCA46, SW48 and C10 cell lines. Tubulin was used as a loading control. Results are plotted as the average ± SD of all the biological replicates and were normalized to the HCA46 cell line ( n = 3). b Colony formation assay of CRC cell lines HCA46, SW48 and C10.Results are plotted as the average ± SD of all the biological replicates. ** p < 0.01 ( n = 3), one-way ANOVA. c ALDH1 relative activity in CRC cell lines HCA46, SW48 and C10. Results were normalized to the cetuximab-sensitive HCA46 cell line. Results are plotted as the average ± SD of all the biological replicates. ** p < 0.01, *** p < 0.001, one-way ANOVA. d Western blot illustrating c-MET expression in SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results were normalized to the YAP1-trasduced cell lines. e Colony formation assay of SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results are plotted as the average ± SD of all the biological replicates, ** p < 0.01 ( n = 3), one-way ANOVA. f ALDH1 relative activity in SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results were normalized to the empty vector condition in each cell line. Results are plotted as the average ± SD of all the biological replicates, * p < 0.05, ** p < 0.01 ( n = 3), one-way ANOVA. g SOX2 gene expression in SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results were normalized to the empty vector condition in each cell line. Results are plotted as the average ± SD of all the biological replicates * p < 0.05, ** p < 0.01 ( n = 3), one-way ANOVA.

Article Snippet: Human CRC cell line HCA46 was obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Western Blot, Control, Colony Assay, Activity Assay, Expressing, Transduction, Plasmid Preparation, Gene Expression

Journal: Aging (Albany NY)

Article Title: A pair of primary colorectal cancer-derived and corresponding synchronous liver metastasis-derived organoid cell lines

doi: 10.18632/aging.205595

Figure Lengend Snippet: Background information of different CRC cell lines.

Article Snippet: By comparison with other CRC cell lines , researchers who are interested in CRC, metastatic CRC, and the corresponding treatments could obtain these cell lines from the CCTCC (No. C202218, C202219; WDPP011, WDPP010) or from our laboratory upon reasonable request.

Techniques: Derivative Assay, Generated, Mutagenesis

Journal: American Journal of Human Genetics

Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction

doi: 10.1016/j.ajhg.2019.05.004

Figure Lengend Snippet: ATF1 Promotes Cell Proliferation and Xenograft Tumor Growth in CRC by Affecting Cell Apoptosis

Article Snippet: A modified ATF1 knockout CRC HCT116 cell line was generated by CRISPR-Cas9 technology (Genloci Biotechnologies).

Techniques:

Journal: American Journal of Human Genetics

Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction

doi: 10.1016/j.ajhg.2019.05.004

Figure Lengend Snippet: Functional Genomic Screening Reveals that ATF1 Is an Oncogene in CRC

Article Snippet: A modified ATF1 knockout CRC HCT116 cell line was generated by CRISPR-Cas9 technology (Genloci Biotechnologies).

Techniques: Functional Assay

Journal: American Journal of Human Genetics

Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction

doi: 10.1016/j.ajhg.2019.05.004

Figure Lengend Snippet: SP1 and GATA3 preferentially bind to the dbSNP: rs61926301[T] and dbSNP: rs7959129[T] alleles at the ATF1 promoter and first intron region, respectively

Article Snippet: A modified ATF1 knockout CRC HCT116 cell line was generated by CRISPR-Cas9 technology (Genloci Biotechnologies).

Techniques:

Journal: American Journal of Human Genetics

Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction

doi: 10.1016/j.ajhg.2019.05.004

Figure Lengend Snippet: Transcription Factors SP1 and GATA3 Correlate with ATF1 Expression in an Allele-Specific Manner

Article Snippet: A modified ATF1 knockout CRC HCT116 cell line was generated by CRISPR-Cas9 technology (Genloci Biotechnologies).

Techniques: Expressing

Journal: American Journal of Human Genetics

Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction

doi: 10.1016/j.ajhg.2019.05.004

Figure Lengend Snippet: rs61926301 and rs7959129 Synergistically Facilitate ATF1 Transcriptional Activity Mediated by the Transcription Factors SP1 and GATA3

Article Snippet: A modified ATF1 knockout CRC HCT116 cell line was generated by CRISPR-Cas9 technology (Genloci Biotechnologies).

Techniques: Activity Assay

Journal: American Journal of Human Genetics

Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction

doi: 10.1016/j.ajhg.2019.05.004

Figure Lengend Snippet: Risk Alleles of rs61926301 and rs7959129 Facilitate a Promoter-Enhancer Interaction Mediated by SP1 and GATA3 to Upregulate ATF1 Expression

Article Snippet: A modified ATF1 knockout CRC HCT116 cell line was generated by CRISPR-Cas9 technology (Genloci Biotechnologies).

Techniques: Expressing

Journal: American Journal of Human Genetics

Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction

doi: 10.1016/j.ajhg.2019.05.004

Figure Lengend Snippet: ATF1 Activates a Panel of Genes Associated with Apoptosis, Wnt, TGF-β, and MAPK Pathways and Facilitates the Early Onset of CRC

Article Snippet: A modified ATF1 knockout CRC HCT116 cell line was generated by CRISPR-Cas9 technology (Genloci Biotechnologies).

Techniques:

Journal: American Journal of Human Genetics

Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction

doi: 10.1016/j.ajhg.2019.05.004

Figure Lengend Snippet: Graphical Representation of the Regulation and Function of ATF1 in CRC

Article Snippet: A modified ATF1 knockout CRC HCT116 cell line was generated by CRISPR-Cas9 technology (Genloci Biotechnologies).

Techniques:

Journal: Communications Biology

Article Title: KCTD15 acts as an anti-tumor factor in colorectal cancer cells downstream of the demethylase FTO and the m6A reader YTHDF2

doi: 10.1038/s42003-024-05880-9

Figure Lengend Snippet: a The mRNA expression of KCTD15 mRNA was determined by qRT-PCR in HCT116 and LoVo cells. b KCTD15 mRNA stability at different time points was measured by qRT-PCR. c Primers were designed to amplify the fragments (sequences 1 and 2) near m6A positions of KCTD15. d Binding of FTO to KCTD15 RNA was measured by RIP-PCR. e MeRIP-qPCR was conducted to analyze the m6A demethylation of KCTD15 RNA. f To further explore the how m6A modification affected KCTD15 mRNA expression, base A within the m6A motif was replaced by base C. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. n = 3 per group.

Article Snippet: HCT116 and LoVo CRC cell lines were purchased from iCell (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Modification

Journal: Communications Biology

Article Title: KCTD15 acts as an anti-tumor factor in colorectal cancer cells downstream of the demethylase FTO and the m6A reader YTHDF2

doi: 10.1038/s42003-024-05880-9

Figure Lengend Snippet: a The effects of YTHDF1, 2, and 3 on KCTD15 mRNA levels were measured by qRT-PCR in HCT116 and LoVo cells. b Hypothetical mechanisms regarding to FTO/YTHDF2-medidated m6A modification of KCTD15. c Binding of YTHDF2 to KCTD15 RNA was measured by RIP-qPCR. d KCTD15 expression was measured by qRT-PCR and Western Blot. e The protein expression levels of FTO and YTHDF2 were detected by Western Blot. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. n = 3 per group.

Article Snippet: HCT116 and LoVo CRC cell lines were purchased from iCell (Shanghai, China).

Techniques: Quantitative RT-PCR, Modification, Binding Assay, Expressing, Western Blot