Journal: Foods

Article Title: Sea Buckthorn Pericarp Flavonoids Improve Diet-Induced Hyperlipidemia via Coordinated Modulation of Hepatic Lipid Metabolism and Gut Microbiota

doi: 10.3390/foods15061049

Figure Lengend Snippet: TFSP modulates the expression of ACC, FAS, CPT-1α, PPARα and ATGL proteins in the livers of HFD mice. n = 6 per group. Data are presented as mean ± SD. Different letters above bars indicate statistically significant differences ( p < 0.05) by one-way ANOVA followed by LSD post hoc test.

Article Snippet: Peroxisome proliferator-activated receptor alpha (PPARα) primary antibody (Cat. No. A00600-2), carnitine palmitoyltransferase-1 alpha (CPT-1α) primary antibody (Cat. No. A00917-3), acetyl-CoA carboxylase (ACC) primary antibody (Cat. No. M01802-2), fatty acid synthase (FAS) primary antibody (Cat. No. BA0484), adipose triglyceride lipase (ATGL) primary antibody (Cat. No. A01800-1), and GAPDH primary antibody (Cat. No. BM1623) were obtained from BOSTER Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing

Journal: Biomolecules & Therapeutics

Article Title: Dietary Potassium Synergistically Enhances Anti-Obesity Efficacy of Garcinia Cambogia Complex in High-Fat Diet-Induced Obese Mice

doi: 10.4062/biomolther.2025.069

Figure Lengend Snippet: The synergistic effect of potassium supplement on lipid accumulation in liver and adipose tissues. (A) Hematoxylin and eosin (H&E) staining of liver, epididymal white-adipose tissue, and inter-scapular brown adipose tissue. Oil staining of liver sections from the same groups and the quantification of hepatic steatosis. (B) Quantification of lipid droplet area in liver, WAT, and BAT. Values are expressed as mean ± SD (n=5 per group). (C) Western blot analysis of lipid metabolism-related proteins including FAS, CPT1, UCP-1, and PGC1α in liver, WAT, and BAT. β-actin was used as a loading control. The significance of the difference between the four groups was analyzed by one-way ANOVA analysis of variance. * p <0.05 and *** p <0.001 versus HFD group.

Article Snippet: Antibodies used included sterol regulatory element-binding protein 1 (SREBP1, 1:50, Invitrogen, Waltham, MA, USA, cat. No. PA1-337), phosphorylated acetyl-CoA carboxylase1 (p-ACC1, 1:30, Cell Signaling, cat. No. 3661), FAS (1:50, Santa Cruz Biotechnology, cat. No. sc20140), phosphorylated AMP-activated protein kinase (p-AMPK, 1:30, cat. No. 2575), and CPT1 (1:50, Novus Biologicals, cat. No. NB100-53791).

Techniques: Staining, Western Blot, Control

Journal: Biomolecules & Therapeutics

Article Title: Dietary Potassium Synergistically Enhances Anti-Obesity Efficacy of Garcinia Cambogia Complex in High-Fat Diet-Induced Obese Mice

doi: 10.4062/biomolther.2025.069

Figure Lengend Snippet: Inhibition of lipogenesis by potassium supplementation through the IGF1R/PI3K/AKT/GSK3β signaling in combination with the GC complex. (A) The immunohistochemical staining of FAS, SREBP1, CPT1 and p-ACC1 in liver sections from each group. (B) Quantification of immunohistochemical (IHC) scoring for positive staining of the four proteins. The expression of lipogenesis-related proteins was significantly decreased by potassium and GC complex compared to the HFD group. Values are expressed as mean ± SD (n=5 per group). (C) Western blot analysis of IGF1R, PI3K, p-AKT, p-GSK3β in liver tissue of each group. β-actin was used as a loading control. The significance of the difference between the four groups was analyzed by one-way ANOVA analysis of variance. * p <0.05 and ** p <0.01 versus HFD group.

Article Snippet: Antibodies used included sterol regulatory element-binding protein 1 (SREBP1, 1:50, Invitrogen, Waltham, MA, USA, cat. No. PA1-337), phosphorylated acetyl-CoA carboxylase1 (p-ACC1, 1:30, Cell Signaling, cat. No. 3661), FAS (1:50, Santa Cruz Biotechnology, cat. No. sc20140), phosphorylated AMP-activated protein kinase (p-AMPK, 1:30, cat. No. 2575), and CPT1 (1:50, Novus Biologicals, cat. No. NB100-53791).

Techniques: Inhibition, Immunohistochemical staining, Staining, Expressing, Western Blot, Control

Journal: Stem Cell Reviews and Reports

Article Title: Gene Expression at the Pluripotency Stage Predicts Pancreatic Endocrine Differentiation in iPSC Clones

doi: 10.1007/s12015-026-11091-y

Figure Lengend Snippet: Validation of group-specific transcriptional differences by RT-qPCR. Box plots showing the expression levels of the top 10 differentially expressed genes (DEGs) distinguishing Gr1 and Gr2 iPSC clones at the pluripotent stage. Statistical significance was defined as P < 0.05 (*), P < 0.01 (**) and P < 0.001 (***). Lines within boxes indicate medians, and whiskers represent variability outside the upper and lower quartiles. Five genes were upregulated in Gr2 compared with Gr1 (GBX2, ZFHX3, UTF1, CNTFR, CPT1A), whereas five were downregulated (PDGFRB, TNNT2, AFP, IDO1, ACBD7)

Article Snippet: Quantitative real-time PCR (RT-qPCR) was performed using TaqMan Gene Expression Assays (Applied Biosystems) for the selected differentially expressed genes (DEGs): upregulated in Gr2 : GBX2 (Hs00230965_m1), ZFHX3 (Hs00199344_m1), UTF1 (Hs00864535_s1), CNTFR (Hs00181798_m1), CPT1A (Hs00912671_m1). downregulated in Gr2 : PDGFRB (Hs01019589_m1), TNNT2 (Hs00943911_m1), AFP (Hs01040598_m1), IDO1 (Hs00984148_m1), and ACBD7 (Hs00744676_s1).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Clone Assay

Journal: Cell Reports Medicine

Article Title: Activation of hepatic adenosine A1 receptor ameliorates MASH via inhibiting SREBPs maturation

doi: 10.1016/j.xcrm.2024.101477

Figure Lengend Snippet: Liver-specific A 1 R knockout exaggerates MAFL and MASH in mice (A–F) Control mice ( A 1 R Flox/Flox without Cre, Flox) and liver-specific A 1 R knockout mice ( A 1 R Liver−/− , LKO) were fed an HFD (60 kcal%) for 16 weeks (n = 6). (A) Diagram of experimental design. (B) Representative image of liver and liver H&E staining. (C) Hepatic steatosis scores and liver index. (D) Quantification of hepatic triglycerides (TGs). (E) The level of serum alanine aminotransferase (ALT). (F) Relative protein expression of CPT1α, CD36, FASN, and ACC. (G–K) Flox mice and LKO mice were fed a calorie-restricted HFD (CD-HFD) for 9 weeks (n = 5∼7). (G) Diagram of experimental design. (H) H&E, F4/80, and Sirius red staining of liver sections, with histological evaluation. (I) Liver injury indicators, including serum ALT and AST. (J) mRNA expression in liver. Results are normalized for 18S . (K) Quantification of hepatic triglycerides (TGs). (L) Relative protein expression of α-SMA, pP65, P65, and IκB. GAPDH functioned as a reference protein. Results are representative of one biological replicate. Data are depicted as mean ± SEM. Student’s unpaired t test; ∗p < 0.05, ∗∗p < 0.01.

Article Snippet: CPT1 antibody , Proteintech, USA , ag7202.

Techniques: Knock-Out, Control, Staining, Expressing

Journal: Cell Reports Medicine

Article Title: Activation of hepatic adenosine A1 receptor ameliorates MASH via inhibiting SREBPs maturation

doi: 10.1016/j.xcrm.2024.101477

Figure Lengend Snippet: Liver-specific A 1 R overexpression protects mice from MAFL and MASH in mice (A–F) C57BL/6J mice were inoculated with vehicle-AAV (vehicle) or A 1 R -overexpression-AAV (A 1 R Liver OE ) intravenously, and then were fed an HFD for 12 weeks (n = 5∼9). (A) Diagram of experimental design. (B) Representative image of liver and liver H&E staining. (C) Hepatic steatosis scores. (D) Quantification of hepatic TG. (E) Liver injury indicators, including serum ALT and AST. (F) Relative protein expression of CPT1α, CD36, FASN, and ACC. (G–K) Vehicle mice and A 1 R Liver OE mice were fed a CD-HFD for 9 weeks (n = 10). (G) Diagram of experimental design. (H) H&E (n = 10), F4/80 (n = 6), and Sirius red staining (n = 10) of liver sections, with histological evaluation. (I) Liver injury indicators, including serum ALT and AST. (J) mRNA expression in liver. (K) Relative protein expression of α-SMA, pP65, P65, and IκB. Results are representative of one biological replicate. Data are depicted as mean ± SEM. Student’s unpaired t test; ∗p < 0.05, ∗∗p < 0.01.

Article Snippet: CPT1 antibody , Proteintech, USA , ag7202.

Techniques: Over Expression, Staining, Expressing

Journal: Cell Reports Medicine

Article Title: Activation of hepatic adenosine A1 receptor ameliorates MASH via inhibiting SREBPs maturation

doi: 10.1016/j.xcrm.2024.101477

Figure Lengend Snippet:

Article Snippet: CPT1 antibody , Proteintech, USA , ag7202.

Techniques: Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Activity Assay, Staining, SYBR Green Assay, Lysis, Protein Extraction, Cholesterol Assay, ALP Assay, In Situ, Fluorescence, Immunoprecipitation, Membrane, Purification, Expressing, Software