cpsf7 Search Results


mouse α cpsf7  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology mouse α cpsf7
Fig. 1. Heterozygous deletion and missense mutations reduce CPSF6 levels in patients. (A) Deletions spanning CPSF6 (orange box) on chromosome 12q15 iden- tified in eight subjects. Dashed lines indicate the minimal overlapping region, which deleted 99% of CPSF6 (subject 8). Mb, megabases. (B) Schematic of the CPSF6 protein showing the RNA binding domain (RBP; orange), the Arg/Ser-rich domain (R/SD) within the nuclear targeting domain (NTD; turquoise), and the CPSF5-interacting domain (blue). Database searches identified 15 individuals with 13 missense variants (indicated by lollipops), which are plotted here for context, but only subjects 9 to 11 (red) were enrolled in this study. Bottom: Evolutionary alignment shows that the three variants in our subjects affect residues that are conserved from zebrafish to humans and have high pathogenicity scores. See fig. S2 for details of the three splicing variants. (C) Representative Western blot and relative quantification shows that subjects 8 and 11 (the only subjects from whom we obtained fibroblasts) have lower CPSF6, CPSF5, <t>CPSF7,</t> and FIP1L1 protein levels than controls. Data were normalized to GAPDH(- glyceraldehyde-3-phosphate dehydrogenase) protein. Data represent means ± SEM from at least four technical and biological replicates compared to healthy age- matched fibroblasts, *P < 0.05, **P < 0.01, and ***P < 0.001.
Mouse α Cpsf7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpsf7/pm36800428-310-30-33?v=Santa+Cruz+Biotechnology
Average 94 stars, based on 1 article reviews
mouse α cpsf7 - by Bioz Stars, 2026-08
94/100 stars
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cpsf5  (Proteintech)
93
Proteintech cpsf5
Fig. 1. Heterozygous deletion and missense mutations reduce CPSF6 levels in patients. (A) Deletions spanning CPSF6 (orange box) on chromosome 12q15 iden- tified in eight subjects. Dashed lines indicate the minimal overlapping region, which deleted 99% of CPSF6 (subject 8). Mb, megabases. (B) Schematic of the CPSF6 protein showing the RNA binding domain (RBP; orange), the Arg/Ser-rich domain (R/SD) within the nuclear targeting domain (NTD; turquoise), and the CPSF5-interacting domain (blue). Database searches identified 15 individuals with 13 missense variants (indicated by lollipops), which are plotted here for context, but only subjects 9 to 11 (red) were enrolled in this study. Bottom: Evolutionary alignment shows that the three variants in our subjects affect residues that are conserved from zebrafish to humans and have high pathogenicity scores. See fig. S2 for details of the three splicing variants. (C) Representative Western blot and relative quantification shows that subjects 8 and 11 (the only subjects from whom we obtained fibroblasts) have lower CPSF6, CPSF5, <t>CPSF7,</t> and FIP1L1 protein levels than controls. Data were normalized to GAPDH(- glyceraldehyde-3-phosphate dehydrogenase) protein. Data represent means ± SEM from at least four technical and biological replicates compared to healthy age- matched fibroblasts, *P < 0.05, **P < 0.01, and ***P < 0.001.
Cpsf5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpsf7/pm39951376-253-27-28?v=Proteintech
Average 93 stars, based on 1 article reviews
cpsf5 - by Bioz Stars, 2026-08
93/100 stars
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hpa041094  (Atlas Antibodies)
90
Atlas Antibodies hpa041094

Hpa041094, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpsf7/pmc06400501-10-9-6?v=Atlas+Antibodies
Average 90 stars, based on 1 article reviews
hpa041094 - by Bioz Stars, 2026-08
90/100 stars
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pre-mrna cleavage factor i (59 kda subunit) antibody  (Bio-Techne corporation)
90
Bio-Techne corporation pre-mrna cleavage factor i (59 kda subunit) antibody

Pre Mrna Cleavage Factor I (59 Kda Subunit) Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpsf7/bio-techne+corporation___nb100-61600?v=Bio-Techne+corporation
Average 90 stars, based on 1 article reviews
pre-mrna cleavage factor i (59 kda subunit) antibody - by Bioz Stars, 2026-08
90/100 stars
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ad-m-cpsf7-shrna  (Vector Biolabs)
N/A
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human cpsf7 gene orf cdna clone in cloning vector  (Sino Biological)
N/A
Full length Clone DNA of Human cleavage and polyadenylation specific factor 7
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cpsf7 mouse qpcr primer pair  (OriGene)
N/A
qSTAR qPCR primer pairs against Mus musculus gene Cpsf7
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cpsf7 nm 024811 human tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Human cleavage and polyadenylation specific factor 7 59kDa CPSF7 transcript variant 1 Myc DDK tagged
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cpsf7 nm 001136040 human tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Human cleavage and polyadenylation specific factor 7 59kDa CPSF7 transcript variant 2 Myc DDK tagged
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human cpsf7 knockout cell line  (AcceGen Biotechnology)
N/A
Human CPSF7 knockout cell line is edited by CRISPR/Cas9 technology.
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cpsf7 (m)-pr  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of CPSF7 gene silencing results, individual duplex components or plasmids are also available upon request. Suitable
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cpsf7 nm 001014245 rat tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Cpsf7 Myc DDK tagged ORF Rat similar to RIKEN cDNA 5730453I16 RGD1305441 10 ug
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Image Search Results


Fig. 1. Heterozygous deletion and missense mutations reduce CPSF6 levels in patients. (A) Deletions spanning CPSF6 (orange box) on chromosome 12q15 iden- tified in eight subjects. Dashed lines indicate the minimal overlapping region, which deleted 99% of CPSF6 (subject 8). Mb, megabases. (B) Schematic of the CPSF6 protein showing the RNA binding domain (RBP; orange), the Arg/Ser-rich domain (R/SD) within the nuclear targeting domain (NTD; turquoise), and the CPSF5-interacting domain (blue). Database searches identified 15 individuals with 13 missense variants (indicated by lollipops), which are plotted here for context, but only subjects 9 to 11 (red) were enrolled in this study. Bottom: Evolutionary alignment shows that the three variants in our subjects affect residues that are conserved from zebrafish to humans and have high pathogenicity scores. See fig. S2 for details of the three splicing variants. (C) Representative Western blot and relative quantification shows that subjects 8 and 11 (the only subjects from whom we obtained fibroblasts) have lower CPSF6, CPSF5, CPSF7, and FIP1L1 protein levels than controls. Data were normalized to GAPDH(- glyceraldehyde-3-phosphate dehydrogenase) protein. Data represent means ± SEM from at least four technical and biological replicates compared to healthy age- matched fibroblasts, *P < 0.05, **P < 0.01, and ***P < 0.001.

Journal: Science advances

Article Title: Alternative polyadenylation alters protein dosage by switching between intronic and 3'UTR sites.

doi: 10.1126/sciadv.ade4814

Figure Lengend Snippet: Fig. 1. Heterozygous deletion and missense mutations reduce CPSF6 levels in patients. (A) Deletions spanning CPSF6 (orange box) on chromosome 12q15 iden- tified in eight subjects. Dashed lines indicate the minimal overlapping region, which deleted 99% of CPSF6 (subject 8). Mb, megabases. (B) Schematic of the CPSF6 protein showing the RNA binding domain (RBP; orange), the Arg/Ser-rich domain (R/SD) within the nuclear targeting domain (NTD; turquoise), and the CPSF5-interacting domain (blue). Database searches identified 15 individuals with 13 missense variants (indicated by lollipops), which are plotted here for context, but only subjects 9 to 11 (red) were enrolled in this study. Bottom: Evolutionary alignment shows that the three variants in our subjects affect residues that are conserved from zebrafish to humans and have high pathogenicity scores. See fig. S2 for details of the three splicing variants. (C) Representative Western blot and relative quantification shows that subjects 8 and 11 (the only subjects from whom we obtained fibroblasts) have lower CPSF6, CPSF5, CPSF7, and FIP1L1 protein levels than controls. Data were normalized to GAPDH(- glyceraldehyde-3-phosphate dehydrogenase) protein. Data represent means ± SEM from at least four technical and biological replicates compared to healthy age- matched fibroblasts, *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Antibodies used for all the Western blot experiments were as follows: rabbit α-CPSF6 [1:1000 (Bethyl Laboratories, TX, catalog no. A301-356A)], mouse α-NUDT21 (2203C3) [1:500 (Santa Cruz, TX, catalog no. sc-81109)], mouse α-CPSF7 [1:500 (Santa Cruz, TX, catalog no. sc-393880)], rabbit α-FIP1L1 [1:500 (A301461A, Bethyl Laboratories, TX)], rabbit α-SCAF4 [1:1000 (Bethyl Laboratories, TX, catalog no. A303-951A)], and mouse α–glyceraldehyde-3-phosphate dehydrogenase [1:10,000 (Millipore, catalog no. CB1001)]. qPCR in human cells and zebrafish For human cells, we collected cells as described above for Western blot.

Techniques: RNA Binding Assay, Western Blot, Quantitative Proteomics

Journal: eLife

Article Title: HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex

doi: 10.7554/eLife.41800

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-hCPSF7 , Atlas Antibodies Cat# HPA041094 , RRID: AB_10794187 , IF(1:200).

Techniques: Enzyme-linked Immunosorbent Assay, Imaging, Reverse Transcription, Recombinant, Plasmid Preparation, Expressing, shRNA, Control, Software