cpsf100 Search Results


cpsf 100  (Bethyl)
94
Bethyl cpsf 100
Cpsf 100, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpsf100/CPSF100+Antibody/pmc03209774-447-0-12
Average 94 stars, based on 1 article reviews
cpsf 100 - by Bioz Stars, 2026-11
94/100 stars
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cpsf2  (Proteintech)
93
Proteintech cpsf2
Cpsf2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpsf100/CPSF2+Antibody/pm38503731-367-15-16
Average 93 stars, based on 1 article reviews
cpsf2 - by Bioz Stars, 2026-11
93/100 stars
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anti cpsf2  (ABclonal Biotechnology)
93
ABclonal Biotechnology anti cpsf2
a , b Heatmap showing total ( a ) and LENG8 target genes ( b ) with shortened or lengthened 3′UTR in three types of germ cells. Line plot on the top showing the mean PDUI in spermatogonia (SG), spermatocytes (SC), and round spermatids (RS). c – f Immunofluorescence images of the relationship between CPSF4 (green) and LENG8 (magenta) ( c ), LENG8 (green) and FIP1L1 (magenta) ( d ), LENG8 (magenta) and YTHDC1 (green) ( e ), LENG8 (magenta) and PABPN1(green) ( f ) in spermatocytes (top) and round spermatids (bottom). Nuclei were counterstained with DAPI (blue). Middle: magnified views of the areas enclosed by dashed boxes. Right: Pearson correlation coefficient showing co-localization patterns between LENG8 and CPSF4, FIP1L1, YTHDC1, and PABPN1. Data are presented as mean ± SEM, n = 20 LENG8 granules from three biologically independent experiments. P -values were determined by two-sided unpaired Student’s t test. g Heatmap illustrating the RNA level, ribosome-protected fragments (RPFs, which correlate with translational efficiency) and protein abundance of targets in four clusters across spermatogonia, spermatocytes and round spermatids. h GO-term enrichment analysis of LENG8 targets in testes, Statistical significance was determined by a modified Fisher′s exact test (EASE score). i Model for LENG8 granule-mediated APA regulation. LENG8 granules mediate the recruitment of CPA factors and facilitate CPA complex assembly at pPASs in a YTHDC1-m ⁶ A dependent manner, ultimately enhancing the usage of these sites. Source data are provided as a Source Data file.
Anti Cpsf2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpsf100/CPSF2+Rabbit+pAb/pmc13672340-549-137-138
Average 93 stars, based on 1 article reviews
anti cpsf2 - by Bioz Stars, 2026-11
93/100 stars
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ha-cpsf100-fl cdna  (Promega)
90
Promega ha-cpsf100-fl cdna
a , b Heatmap showing total ( a ) and LENG8 target genes ( b ) with shortened or lengthened 3′UTR in three types of germ cells. Line plot on the top showing the mean PDUI in spermatogonia (SG), spermatocytes (SC), and round spermatids (RS). c – f Immunofluorescence images of the relationship between CPSF4 (green) and LENG8 (magenta) ( c ), LENG8 (green) and FIP1L1 (magenta) ( d ), LENG8 (magenta) and YTHDC1 (green) ( e ), LENG8 (magenta) and PABPN1(green) ( f ) in spermatocytes (top) and round spermatids (bottom). Nuclei were counterstained with DAPI (blue). Middle: magnified views of the areas enclosed by dashed boxes. Right: Pearson correlation coefficient showing co-localization patterns between LENG8 and CPSF4, FIP1L1, YTHDC1, and PABPN1. Data are presented as mean ± SEM, n = 20 LENG8 granules from three biologically independent experiments. P -values were determined by two-sided unpaired Student’s t test. g Heatmap illustrating the RNA level, ribosome-protected fragments (RPFs, which correlate with translational efficiency) and protein abundance of targets in four clusters across spermatogonia, spermatocytes and round spermatids. h GO-term enrichment analysis of LENG8 targets in testes, Statistical significance was determined by a modified Fisher′s exact test (EASE score). i Model for LENG8 granule-mediated APA regulation. LENG8 granules mediate the recruitment of CPA factors and facilitate CPA complex assembly at pPASs in a YTHDC1-m ⁶ A dependent manner, ultimately enhancing the usage of these sites. Source data are provided as a Source Data file.
Ha Cpsf100 Fl Cdna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpsf100/ha+cpsf+100+cdna/10__1128_slash_mcb__19__8__5707-121-0-6
Average 90 stars, based on 1 article reviews
ha-cpsf100-fl cdna - by Bioz Stars, 2026-11
90/100 stars
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1h, 15n and 13c resonance assignments of a minimal cpsf73-cpsf100 c-terminal heterodimer  (Inserm Transfert)
90
Inserm Transfert 1h, 15n and 13c resonance assignments of a minimal cpsf73-cpsf100 c-terminal heterodimer
a , b Heatmap showing total ( a ) and LENG8 target genes ( b ) with shortened or lengthened 3′UTR in three types of germ cells. Line plot on the top showing the mean PDUI in spermatogonia (SG), spermatocytes (SC), and round spermatids (RS). c – f Immunofluorescence images of the relationship between CPSF4 (green) and LENG8 (magenta) ( c ), LENG8 (green) and FIP1L1 (magenta) ( d ), LENG8 (magenta) and YTHDC1 (green) ( e ), LENG8 (magenta) and PABPN1(green) ( f ) in spermatocytes (top) and round spermatids (bottom). Nuclei were counterstained with DAPI (blue). Middle: magnified views of the areas enclosed by dashed boxes. Right: Pearson correlation coefficient showing co-localization patterns between LENG8 and CPSF4, FIP1L1, YTHDC1, and PABPN1. Data are presented as mean ± SEM, n = 20 LENG8 granules from three biologically independent experiments. P -values were determined by two-sided unpaired Student’s t test. g Heatmap illustrating the RNA level, ribosome-protected fragments (RPFs, which correlate with translational efficiency) and protein abundance of targets in four clusters across spermatogonia, spermatocytes and round spermatids. h GO-term enrichment analysis of LENG8 targets in testes, Statistical significance was determined by a modified Fisher′s exact test (EASE score). i Model for LENG8 granule-mediated APA regulation. LENG8 granules mediate the recruitment of CPA factors and facilitate CPA complex assembly at pPASs in a YTHDC1-m ⁶ A dependent manner, ultimately enhancing the usage of these sites. Source data are provided as a Source Data file.
1h, 15n And 13c Resonance Assignments Of A Minimal Cpsf73 Cpsf100 C Terminal Heterodimer, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpsf100/1h++15n+and+13c+resonance+assignments+of+a+minimal+cpsf73+cpsf100+c+terminal+heterodimer/pm36723825-85-20-33
Average 90 stars, based on 1 article reviews
1h, 15n and 13c resonance assignments of a minimal cpsf73-cpsf100 c-terminal heterodimer - by Bioz Stars, 2026-11
90/100 stars
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cpsf100 ihc antibody  (Bethyl)
N/A
Rabbit anti-CPSF100 IHC Antibody, Affinity Purified
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Image Search Results


a , b Heatmap showing total ( a ) and LENG8 target genes ( b ) with shortened or lengthened 3′UTR in three types of germ cells. Line plot on the top showing the mean PDUI in spermatogonia (SG), spermatocytes (SC), and round spermatids (RS). c – f Immunofluorescence images of the relationship between CPSF4 (green) and LENG8 (magenta) ( c ), LENG8 (green) and FIP1L1 (magenta) ( d ), LENG8 (magenta) and YTHDC1 (green) ( e ), LENG8 (magenta) and PABPN1(green) ( f ) in spermatocytes (top) and round spermatids (bottom). Nuclei were counterstained with DAPI (blue). Middle: magnified views of the areas enclosed by dashed boxes. Right: Pearson correlation coefficient showing co-localization patterns between LENG8 and CPSF4, FIP1L1, YTHDC1, and PABPN1. Data are presented as mean ± SEM, n = 20 LENG8 granules from three biologically independent experiments. P -values were determined by two-sided unpaired Student’s t test. g Heatmap illustrating the RNA level, ribosome-protected fragments (RPFs, which correlate with translational efficiency) and protein abundance of targets in four clusters across spermatogonia, spermatocytes and round spermatids. h GO-term enrichment analysis of LENG8 targets in testes, Statistical significance was determined by a modified Fisher′s exact test (EASE score). i Model for LENG8 granule-mediated APA regulation. LENG8 granules mediate the recruitment of CPA factors and facilitate CPA complex assembly at pPASs in a YTHDC1-m ⁶ A dependent manner, ultimately enhancing the usage of these sites. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: LENG8 granule regulates alternative polyadenylation in mammals

doi: 10.1038/s41467-026-77634-6

Figure Lengend Snippet: a , b Heatmap showing total ( a ) and LENG8 target genes ( b ) with shortened or lengthened 3′UTR in three types of germ cells. Line plot on the top showing the mean PDUI in spermatogonia (SG), spermatocytes (SC), and round spermatids (RS). c – f Immunofluorescence images of the relationship between CPSF4 (green) and LENG8 (magenta) ( c ), LENG8 (green) and FIP1L1 (magenta) ( d ), LENG8 (magenta) and YTHDC1 (green) ( e ), LENG8 (magenta) and PABPN1(green) ( f ) in spermatocytes (top) and round spermatids (bottom). Nuclei were counterstained with DAPI (blue). Middle: magnified views of the areas enclosed by dashed boxes. Right: Pearson correlation coefficient showing co-localization patterns between LENG8 and CPSF4, FIP1L1, YTHDC1, and PABPN1. Data are presented as mean ± SEM, n = 20 LENG8 granules from three biologically independent experiments. P -values were determined by two-sided unpaired Student’s t test. g Heatmap illustrating the RNA level, ribosome-protected fragments (RPFs, which correlate with translational efficiency) and protein abundance of targets in four clusters across spermatogonia, spermatocytes and round spermatids. h GO-term enrichment analysis of LENG8 targets in testes, Statistical significance was determined by a modified Fisher′s exact test (EASE score). i Model for LENG8 granule-mediated APA regulation. LENG8 granules mediate the recruitment of CPA factors and facilitate CPA complex assembly at pPASs in a YTHDC1-m ⁶ A dependent manner, ultimately enhancing the usage of these sites. Source data are provided as a Source Data file.

Article Snippet: Antibodies were used for western blot, IF, and IP: anti-LENG8 (Signalway Antibody, 39875, 1:100 in IF), anti-LENG8 (Bethyl, A304-947A, 1:1000 in WB), anti-LENG8 (our lab, 1:100 in IF and IP), anti-PABPN1 (Beyotime Biotechnology, AG3616, 1:2000 in WB, 1:200 in IF), anti-YTHDC1(Santa Cruz, sc-136428, 1:200 in IF), anti-CPSF4 (Santa Cruz, sc-390643, 1:10 in IF), anti-SYCP3(Santa Cruz, sc-74569, 1:300 in IF), anti-NONO (Santa Cruz, sc-166702, 1:10 in IF), anti-DDX25 (Santa Cruz, sc-166289, 1:200 in IF), anti-CPSF4 (Proteintech, 15023-1-AP, 1:1000 in WB), anti-COIL (Proteintech, 67218-1-Ig, 1:100 in IF), anti-YTHDC1 (ABclonal, A22992, 1:1000 in WB), anti-FIP1L1(ABclonal, A7138, 1:1000 in WB, 1:100 in IF), anti-GFP (Abways, AB0005, 1:2000 in WB), anti-β-actin (Abways, Ab2001, 1:10000 in WB), anti-Myc (Abways, AB0001, 1:2000 in WB), anti-Flag (Abways, AB0008, 1:2000 in WB), anti-HA (Cell Signaling Technology, 3724, 1:2000 in WB), anti-SC35 (Abcam, Ab11826, 1:200 in IF), anti-CPSF2 (ABclonal, A9297, 1:1000 in WB, 1:100 in IF), anti-CPSF6(Santa Cruz, sc-376228, 1:100 in IF), anti-SC35 (Santa Cruz, sc-53518, 1:100 in IF), Mouse polyclonal anti-IgG (Merck, 12-371), Rabbit polyclonal anti-IgG (Merck, 12-370), HRP, Goat Anti-Mouse IgG (Abbkine, A21010, 1:10000 in WB), HRP, Goat Anti-Mouse IgG LCS (Abbkine, A25012, 1:10000 in WB), HRP, Goat Anti-Rabbit IgG (Abbkine, A21020, 1:10000 in WB), Alexa Fluor 488 AffiniPure Donkey Anti-MOUSE (JACKSON ImmunoResearch, 715-545-150, 1:500 in IF), Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit (JACKSON ImmunoResearch, 711-585-152, 1:500 in IF), IFKine Red Donkey Anti-Mouse (Abbkine, A24411, 1:500 in IF) and IFKine Green Donkey Anti-Rabbit (Abbkine, A24221, 1:500 in IF), ABflo 647-conjugated Goat anti-Mouse IgG (ABclonal, AS059, 1:500 in IF).

Techniques: Immunofluorescence, Quantitative Proteomics, Analysis, Modification, Cell Differentiation, Significance Assay