Journal: Nature Communications

Article Title: Sexual dimorphism in melanocyte stem cell behavior reveals combinational therapeutic strategies for cutaneous repigmentation

doi: 10.1038/s41467-024-45034-3

Figure Lengend Snippet: A Volcano plot of differentially upregulated genes in female (left) and male (right) mice. Genes that belong to Acute Inflammatory Response are highlighted. B Expression level of Ptgs2 in all immune cell subclusters described in Fig. . C Log-fold gene expression changes in cyclooxygenases and select prostaglandin synthesis factors following UVB irradiation in males and females, from bulk mRNA sequencing data. *, **, *** indicates p.adjusts (apeglm shrinkage) are lower than 0.05, 0.01, 0.001, respectively. D Antibody staining for Ptges2, Ptges3, Ptger2, co-stained with F4/80 and DAPI on control skin collected at day 3. Co-localizations are indicated by circle. n = 3 males. E Schematic of Rosa26-CreER; Ptgs2 f/f mouse model, created with BioRender.com. F Representative images of McSC migration and quantification from Cox-2 global knockout mice compared to controls. n = 10 male mice for control (Ctrl), n = 14 male for knockout (KO). Ptgs2 f/+ or Ptgs2 +/+ were used as controls. Statistics: significance calculated by Welch’s t test. Error bar: SEM. Scale bar: 100 µm.

Article Snippet: Antibodies used: F4/80 1:600 (Biolegend, Cat#123101); Ly6G 1:600 (Biolegend, Cat#127601); CD3 1:800 (Biolegend, Cat#100201); Cox-2 1:600 (Cayman, Cat#160106); Ptges2 1:200 (Abclonal, Cat#A7137); Ptges3 1:200 (Abclonal, Cat#A5194); Ptger2 1:500 (Abcam, Cat#ab167171); Cd49f 1:100 (BD Biosciences, Cat#555734); Dct 1:600 (Abcam, Cat#ab221144), together with suitable Alex Fluor secondary antibodies (Abcam, Cat#ab150072, ab150149, ab150152, Fisher, Cat#A21207)

Techniques: Expressing, Gene Expression, Irradiation, Sequencing, Staining, Control, Migration, Knock-Out