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Image Search Results
Journal: Cancer cell
Article Title: The osteogenic niche is a calcium reservoir of bone micrometastases and confers unexpected therapeutic vulnerability
doi: 10.1016/j.ccell.2018.10.002
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Western blots were performed using antibodies against NFAT1(Cell Signalling, 5861S), pS6K(T389) (Cell Signaling, 9234S), pCamKII(T286) (Cell Signalling, 12716S), MeCP2 (Cell Signalling, 3456S), pMeCP2(
Techniques: Luciferase, Recombinant, Expressing, In Vivo, Software
Journal: The Journal of infectious diseases
Article Title: Hepatitis C Virus nonstructural 5A protein inhibits lipopolysaccharide-mediated apoptosis of hepatocytes by decreasing expression of Toll-like receptor 4.
doi: 10.1093/infdis/jir381
Figure Lengend Snippet: Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), TLR9 (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
Article Snippet: 3HCL; 100 lg/mL), TLR3 (poly[I:C]; 50 lg/mL), TLR4 (LPS from Escherichia coli; 5 lg/mL), TLR5 (purified flagellin; 100 lg/mL), TLR6/TLR2 (macrophage-activating lipopeptide 2; 100 lg/mL), TLR7 (Imiquimod [R-837]; 2.5 lg/mL), and
Techniques: Control, Cell Culture, Staining, Saline, Apoptosis Assay