Journal: Regulatory peptides

Article Title: Increased circulating angiotensin-(1-7) protects white adipose tissue against development of a proinflammatory state stimulated by a high-fat diet.

doi: 10.1016/j.regpep.2012.06.009

Figure Lengend Snippet: Fig. 3. Adipose tissue inflammatory markers. Western blot for COX2-epididymal adipose tissue (A), RT-PCR expression of IL-1β‐epididymal adipose tissue (B), SD immunohisto- chemical reaction for IL-1β in epididymal adipose tissue (C), TGR immunohistochemical reaction for IL-1β in epididymal adipose tissue (D), RT-PCR expression of TNF-α‐ epididymal adipose tissue (E), SD immunohistochemical reaction for TNF-α in epididymal adipose tissue (F), TGR immunohistochemical reaction for TNF-α in epididymal adipose tissue (G). Data are presented as mean±SEM; * pb0.05.

Article Snippet: The blot was probed with COX2 enzyme antibody (Cell Signaling, USA; 1:1000), diluted in 1× blocking buffer, washed in 1× TBS with 1% of Tween 20, and then incubated with either IRDye 800 anti-goat secondary antibody (Rockland Immunochemicals®, Gilbertsville, Pa.) diluted to 1:10,000 in blocking buffer followed by further washes with PBS-Tween 20 and one wash in TBS.

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Expressing, Immunohistochemical staining

Journal: Diabetology & Metabolic Syndrome

Article Title: Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance

doi: 10.1186/s13098-023-01244-1

Figure Lengend Snippet: Antibodies

Article Snippet: MT-CO2 , Rabbit , Cell Signaling Technology , #31,219.

Techniques:

Journal: Diabetology & Metabolic Syndrome

Article Title: Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance

doi: 10.1186/s13098-023-01244-1

Figure Lengend Snippet: Didymin improves mitochondrial function in PBCs. ( A ) MitoTracker Green staining for mitochondrial content in INS-1 cells. Scale bar = 30 μm. (n = 4) ( B ) Western blot analysis of NRF1, TFAM, NDUFB8, SDHB and MTCO2 in INS-1 cells (n = 3). ( C ) Mitochondrial oxygen consumption ratio (OCR) of INS-1 cells (n = 4). ( D ) ROS concentration in INS-1 cells (n = 4). ( E ) Analysis of mitochondrial structure of PBCs in mice by electron microscopy. Scale bar = 0.6 μm. ( F ) Immunofluorescence staining of COX-IV (green) and insulin (red) in pancreas. Scale bar = 20 μm. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001 PA vs. PA + Didymin. # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 control vs. PA.

Article Snippet: MT-CO2 , Rabbit , Cell Signaling Technology , #31,219.

Techniques: Staining, Western Blot, Concentration Assay, Electron Microscopy, Immunofluorescence, Control