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Image Search Results
Journal: Antioxidants
Article Title: Taurine Attenuates Disuse Muscle Atrophy Through Modulation of the xCT-GSH-GPX4 and AMPK-ACC-ACSL4 Pathways
doi: 10.3390/antiox14070847
Figure Lengend Snippet: Taurine inhibits ferroptosis in the gastrocnemius muscle of DMA mice. ( A ) Immuno-histochemical staining for COX2 (blue arrows), a marker of lipid peroxidation, showing increased expression in DMA mice, which was significantly reduced by taurine treatment. Scale bar: 100 μm. Total magnification: 200×. ( B ) MDA levels, a key marker of lipid peroxidation, showing a significant increase in DMA mice and a marked reduction after taurine supplementation (biological replicates, n = 4). ( C ) Iron accumulation in the gastrocnemius muscle of DMA nice, a characteristic feature of ferroptosis, was significantly increased in DMA mice and attenuated by taurine treatment (biological replicates, n = 4). ( D ) Total GSH level was significantly decreased in the gastrocnemius muscle of DMA mice and increased by taurine treatment (biological replicates, n = 9). ( E – G ) Western blot analysis ( E ) and grayscale analysis of NRF2 ( F ) and xCT ( G ). The expression of NRF2 and xCT was significantly reduced in DMA mice, whereas taurine treatment restored their levels (biological replicates, n = 3). ( H , I ) Western blot analysis ( H ) and grayscale analysis of GPX4 ( I ), the key regulators in the ferroptosis defense system. The expression of GPX4 was significantly reduced in DMA mice, whereas taurine treatment restored their levels (biological replicates, n = 3). ( J ) The enzymatic activity of GPx showed that DMA resulted in decreased GPx activity, whereas taurine improved it (biological replicates, n = 4). Data are expressed as mean ± SEM and analyzed using one-way ANOVA with Tukey’s post hoc test. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 vs. DMA.
Article Snippet: For immunohistochemistry,
Techniques: Staining, Marker, Expressing, Western Blot, Activity Assay