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Image Search Results
Journal: Mediators of Inflammation
Article Title: Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells
doi: 10.1155/2020/4185273
Figure Lengend Snippet: (a) Expression of COX2 in the presence and absence of F. alocis (OD 660 : 0.1) in HGF-1 cells at 1 d and 2 d, as analyzed by qPCR. Mean ± SEM ( n = 18). ∗ Significant ( p < 0.05) difference between groups. (b) Stimulation of COX2 expression by various concentrations of F. alocis (OD 660 : 0.05, 0.1, and 0.2) in HGF-1 cells at 1 d, as analyzed by qPCR. Unstimulated cells served as the control. Mean ± SEM ( n = 6). ∗ Significantly ( p < 0.05) different from control. (c) COX2 protein level in lysates of HGF-1 cells in the presence and absence of F. alocis (OD 660 : 0.1) at 1 d, as analyzed by ELISA. Mean ± SEM ( n = 12). ∗ Significant ( p < 0.05) difference between groups. (d) Expression of COX2 in the presence and absence of TNF α (1 ng/ml) in HGF-1 cells at 1 d and 2 d, as analyzed by qPCR. Mean ± SEM ( n = 26). ∗ Significant ( p < 0.05) difference between groups.
Article Snippet: HGF-1 or THP-1 cell lysates were used to evaluate the levels of COX2 by using commercially available sandwich enzyme-linked
Techniques: Expressing, Enzyme-linked Immunosorbent Assay
Journal: Mediators of Inflammation
Article Title: Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells
doi: 10.1155/2020/4185273
Figure Lengend Snippet: (a) Expression of COX2 in the presence and absence of F. alocis (OD 660 : 0.1) in THP-1 cells at 1 d and 2 d, as analyzed by qPCR. Mean ± SEM ( n = 18). ∗ Significant ( p < 0.05) difference between groups. (b) Stimulation of COX2 expression by various concentrations of F. alocis (OD 660 : 0.05, 0.1, and 0.2) in THP-1 cells at 1 d, as analyzed by qPCR. Unstimulated cells served as the control. Mean ± SEM ( n = 18). ∗ Significantly ( p < 0.05) different from the control. (c) COX2 protein level in lysates of THP-1 cells in the presence and absence of F. alocis (OD 660 : 0.1) at 1 d, as analyzed by ELISA. Mean ± SEM ( n = 18). ∗ Significant ( p < 0.05) difference between groups. (d) Expression of COX2 in the presence and absence of TNF α (1 ng/ml) in THP-1 cells at 1 d and 2 d, as analyzed by qPCR. Mean ± SEM ( n = 12). ∗ Significant ( p < 0.05) difference between groups.
Article Snippet: HGF-1 or THP-1 cell lysates were used to evaluate the levels of COX2 by using commercially available sandwich enzyme-linked
Techniques: Expressing, Enzyme-linked Immunosorbent Assay
Journal: Mediators of Inflammation
Article Title: Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells
doi: 10.1155/2020/4185273
Figure Lengend Snippet: COX2 gene expression in response to F. alocis (OD 660 : 0.1) in the presence and absence of anti-TLR2 blocking antibody in HGF-1 (a) and THP-1 (b) cells at 1 d, as analyzed by qPCR. Mean ± SEM ( n = 3). ∗ Significant ( p < 0.05) difference between groups. COX2 gene expression in response to F. alocis (OD 660 : 0.1) in the presence and absence of specific inhibitors against MEK1/MEK2 (U0126; 10 μ M), p38 (SB203580; 10 μ M), NF- κ B (PDTC; 10 μ M), and JNK (SP600125; 10 μ M) in HGF-1 (c) and THP-1 (d) cells at 1 d, as analyzed by qPCR. Mean ± SEM ( n = 3). ∗ Significant ( p < 0.05) difference between groups.
Article Snippet: HGF-1 or THP-1 cell lysates were used to evaluate the levels of COX2 by using commercially available sandwich enzyme-linked
Techniques: Expressing, Blocking Assay