Journal: Frontiers in Marine Science

Article Title: Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos

doi: 10.3389/fmars.2022.880103

Figure Lengend Snippet: FIGURE 1 | Immunofluorescence staining of gill filaments of milkfish stained with anti-cytochrome c oxidase (COX IV, green; (A, D), citrate synthase (CS, red; (B), sodium-potassium ATPase a1 subunit (NKAa1, red; (E), glycogen phosphorylase (GP, red; (G), sodium-potassium ATPase a subunit (NKA, green; (G, H), and glucose transporter 1 (GLUT1, red; (H) antibodies. Immunoreactions for the merged images (C) and (F) are shown from COX IV (A) with CS (B), and COX IV (D) with NKAa1 (E), respectively. F, filament; L, lamella. Scale bar, 50 mm.

Article Snippet: The following primary antibodies were used in this study: (1) Na+, K+-ATPase a1 subunit (a6F, DSHB, Iowa City, IA, USA); (2) Na+, K+-ATPase a subunit (a5, DSHB, Iowa City, IA, USA); (3) citrate synthase (sc-390693, Santa Cruz Biotechnology, Dallas, TX, USA); (4) COX IV (#4850, Cell Signaling, Beverly, MA, USA); (5) b-actin (GTX109639, Genetex, Irvine, CA, USA); (6) glycogen phosphorylase (GTX124390, Genetex); and (7) glucose transporter 1 (ab128033, Abcam; Figure S1).

Techniques: Staining

Journal: Frontiers in Marine Science

Article Title: Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos

doi: 10.3389/fmars.2022.880103

Figure Lengend Snippet: FIGURE 4 | Relative protein abundance of branchial citrate synthase (CS) and cytochrome c oxidase IV (COX IV) in freshwater (FW)- and seawater (SW)-acclimated milkfish at 28°C (white bar) and 18°C (striped bar). Representative immunoblots of CS (A) and COX IV (B) show a single immunoreactive band. The single immunoreactive band of b-actin at 42 kDa was used as the loading control. Different letters in the FW milkfish (a, b) and SW milkfish (a’, b’) indicate significant differences between the normal and low-temperature groups. The x and y indicate significant differences between the FW and SW groups at 28°C or 18°C. Values represent the mean ± standard error of the mean (SEM), n = 9, P < 0.05.

Article Snippet: The following primary antibodies were used in this study: (1) Na+, K+-ATPase a1 subunit (a6F, DSHB, Iowa City, IA, USA); (2) Na+, K+-ATPase a subunit (a5, DSHB, Iowa City, IA, USA); (3) citrate synthase (sc-390693, Santa Cruz Biotechnology, Dallas, TX, USA); (4) COX IV (#4850, Cell Signaling, Beverly, MA, USA); (5) b-actin (GTX109639, Genetex, Irvine, CA, USA); (6) glycogen phosphorylase (GTX124390, Genetex); and (7) glucose transporter 1 (ab128033, Abcam; Figure S1).

Techniques: Quantitative Proteomics, Western Blot, Control

Journal: Frontiers in Marine Science

Article Title: Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos

doi: 10.3389/fmars.2022.880103

Figure Lengend Snippet: FIGURE 5 | Activity levels of citrate synthase (CS) (A) and cytochrome c oxidase IV (COX IV) (B) in the gills of milkfish acclimated to 28°C (white bar) and 18°C (striped bar). Different letters in the FW milkfish (a, b) and SW milkfish (a’, b’) indicate significant differences between the normal and low-temperature groups. The x and y indicate significant differences between the FW and SW groups at 28°C or 18°C. Values represent the mean ± standard error of the mean (SEM), n = 9, P < 0.05.

Article Snippet: The following primary antibodies were used in this study: (1) Na+, K+-ATPase a1 subunit (a6F, DSHB, Iowa City, IA, USA); (2) Na+, K+-ATPase a subunit (a5, DSHB, Iowa City, IA, USA); (3) citrate synthase (sc-390693, Santa Cruz Biotechnology, Dallas, TX, USA); (4) COX IV (#4850, Cell Signaling, Beverly, MA, USA); (5) b-actin (GTX109639, Genetex, Irvine, CA, USA); (6) glycogen phosphorylase (GTX124390, Genetex); and (7) glucose transporter 1 (ab128033, Abcam; Figure S1).

Techniques: Activity Assay

Journal: Biomedicines

Article Title: Effect of Serping1 siRNA Injection on Dopaminergic Cell Reduction in an MPTP-Induced Parkinson’s Disease Mouse Model

doi: 10.3390/biomedicines14030569

Figure Lengend Snippet: Changes in cyclooxygenase-2 (COX2) and inducible nitric oxide synthase (iNOS) levels in the striatum by injecting Serping1 siRNA. ( A ) Immunoblot results of COX2 and iNOS in the striatum of C, NC, SER1, and NAC groups. ( B ) The immunoblot of COX2 and iNOS is shown in a graph. COX2 and iNOS levels increased significantly in the NC group. However, COX2 levels decreased significantly in the SER1 and NAC groups, and iNOS levels also decreased in the SER1 and NAC groups; (COX2, n = 3, F(3,8) = 14.270, ANOVA = 0.001; iNOS, n = 3, F(3,8) = 4.366, ANOVA = 0.042) means ± standard errors, * p < 0.05, ** p < 0.005, # p = 0.067.

Article Snippet: Then, the membranes were incubated with primary antibodies: anti-TH (1:2000; Santa Cruz Biotechnology, Dallas, TX, USA), anti- Serping1 (1:2000; Cloud clone Corp., Katy, TX, USA), anti-β-actin (1:5000; Santa Cruz Biotechnology), anti-pSer129-α-syn (1:1000; FUJIFILM Wako Pure Chemical Corp., Osaka, Japan), anti-cyclooxygenase-2 (COX2) (1:2000; Proteintech group, Rosemont, IL, USA), and anti-nitric oxide synthase (iNOS) (1:2000; Calbiochem, Darmstadt, Germany).

Techniques: Western Blot