Journal: PLOS ONE

Article Title: Evaluation of the Kaira COVID-19/Flu/RSV Detection Kit for detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus: A comparative study with the PowerChek SARS-CoV-2, influenza A&B, RSV Multiplex Real-time PCR Kit

doi: 10.1371/journal.pone.0278530

Figure Lengend Snippet: Analytical specificity evaluation results of the Kaira assay.

Article Snippet: Human coronavirus 229E , ATCC (VR-740D) , Negative.

Techniques: Virus

Journal: Journal of Clinical Microbiology

Article Title: Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications

doi: 10.1128/JCM.02507-16

Figure Lengend Snippet: PEDV multiplex (6-plex) fluorescent microbead-based immunoassay (FMIA) sample/positive-result (S/P) ratios of serum antibody (IgG) responses (means and standard errors [SE]) over time to the 5 recombinant polypeptides (S1 non-S-INDEL, S1 S-INDEL, N, M, and E) and to the whole-virus (WV) antigen in pigs (n = 12 per group) inoculated with PEDV (USA/IN/2013/19338E), TGEV Miller (ATCC VR-1740), TGEV Purdue (ATCC VR-763), PRCV (ATCC VR-2384), or PDCoV (USA/IL/2014) or with a negative control (sham inoculation). Different letters denoted statistical differences (P = ≤0.05). Samples above the estimated S/P cutoff (dashed line) were considered positive.

Article Snippet: PRCV (ATCC VR-2384) , 29 , 29 , 31 , 55 , 30.

Techniques: Multiplex Assay, Recombinant, Virus, Negative Control

Journal: Journal of Clinical Microbiology

Article Title: Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications

doi: 10.1128/JCM.02507-16

Figure Lengend Snippet: Performance of PEDV whole-virus (WV) indirect ELISA on experimental samples of precisely known porcine coronavirus infectious status, i.e., pigs inoculated with PEDV (USA/IN/2013/19338E; n = 12), TGEV Miller (ATCC VR-1740; n = 12), TGEV Purdue (ATCC VR-763; n = 12), PRCV (ATCC VR-2384; n = 12), or PDCoV (USA/IL/2014; n = 12) or with a negative control (sham inoculation; n = 12). (A) Sample/positive result (S/P) ratios of serum antibody (IgG) responses (mean, SE) over time in each inoculation group. (B) Distribution of cumulative ELISA WV IgG sample/positive result (S/P) ratios in serum samples (n = 792 total; n = 132 per group) collected at DPI −7, 0, 3, 7, 10, 14, 17, 21, 28, 35, and 42. Different letters denoted statistical differences (P = ≤0.05). Samples above the estimated S/P cutoff (dashed line) were considered positive.

Article Snippet: PRCV (ATCC VR-2384) , 29 , 29 , 31 , 55 , 30.

Techniques: Virus, Indirect ELISA, Negative Control, Enzyme-linked Immunosorbent Assay

Journal: Journal of Clinical Microbiology

Article Title: Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications

doi: 10.1128/JCM.02507-16

Figure Lengend Snippet: Distribution of cumulative FMIA IgG sample/positive result (S/P) ratios obtained for each recombinant polypeptide (S1 non-S-INDEL, S1 S-INDEL, N, M, or E) on serum samples (n = 792 total; n = 132 per group) collected at DPI −7, 0, 3, 7, 10, 14, 17, 21, 28, 35, and 42 from pigs (n = 72 total; n = 12 per group) inoculated with PEDV (USA/IN/2013/19338E), TGEV Miller (ATCC VR-1740), TGEV Purdue (ATCC VR-763), PRCV (ATCC VR-2384), or PDCoV (USA/IL/2014) or with a negative control (sham inoculation). The FMIA S/P cutoff values estimated for each individual antigen are presented in the graph (dashed line).

Article Snippet: PRCV (ATCC VR-2384) , 29 , 29 , 31 , 55 , 30.

Techniques: Recombinant, Negative Control

Journal: Journal of Clinical Microbiology

Article Title: Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications

doi: 10.1128/JCM.02507-16

Figure Lengend Snippet: Amino acid sequence homology of PEDV recombinant polypeptides S1, N, M, and E compared to homologous regions of PEDV-related porcine coronaviruses TGEV Miller, TGEV Purdue, PRCV, and PDCoV used during experimental inoculation a

Article Snippet: PRCV (ATCC VR-2384) , 29 , 29 , 31 , 55 , 30.

Techniques: Sequencing, Recombinant

Journal: STAR Protocols

Article Title: qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples

doi: 10.1016/j.xpro.2020.100102

Figure Lengend Snippet: Appropriate and Inappropriate qRT-PCR Curves for Samples (A) Representation of curves for N1 gene in one patient sample. Each curve represents one technical replicate (i.e., one well) of the qRT-PCR run. The curve labeled with an asterisk (∗) denotes what one would expect if N1 transcript is not present in the sample. The curve labeled with yellow arrow head represents a spurious curve with a C T value of 5.594 likely due to technical artifact. (B) Technical duplicate curves generated for N1 gene in one positive patient sample. These curves, with C T value of 31.131, are what one would expect if the qRT-PCR run identified SARS-CoV-2 viral genomic RNA present in patient samples.

Article Snippet: Please note however, that it is possible to obtain full-length SARS-CoV-2 genomic RNA from ATCC (Cat. No. VR-1986D) to use in lieu of or addition to the TaqPath COVID-19 Combo Kit positive control.

Techniques: Quantitative RT-PCR, Labeling, Generated

Journal: STAR Protocols

Article Title: qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples

doi: 10.1016/j.xpro.2020.100102

Figure Lengend Snippet:

Article Snippet: Please note however, that it is possible to obtain full-length SARS-CoV-2 genomic RNA from ATCC (Cat. No. VR-1986D) to use in lieu of or addition to the TaqPath COVID-19 Combo Kit positive control.

Techniques: Reverse Transcription, Adhesive, Multiplex Assay, Software, Real-time Polymerase Chain Reaction

Journal: Virus Research

Article Title: Preparation and characterization of a novel monoclonal antibody specific to severe acute respiratory syndrome-coronavirus nucleocapsid protein

doi: 10.1016/j.virusres.2006.07.004

Figure Lengend Snippet: Cross-reactivity of SARS-N mAbs with human coronavirus antigens. (A) The immunofluorescence assay was performed by using SARS-N mAbs on human coronavirus OC43-infected MRC-5 cells. As a positive control, anti-HCoV was used as the mAb against human coronavirus OC43 N protein, and normal mouse serum was used as the negative control. FM and LM indicate fluorescence and light imagery. None of these mAbs showed cross-reactivity with human coronavirus. (B) Cross-reactivity of SARS-N mAbs was examined by antigen-capture ELISA using human coronavirus OC43 lysates (256 HA unit), BrSARS-N protein (500 ng/well) and PBST buffer with 1% BSA as control.

Article Snippet: Human coronavirus OC43 (ATCC, VR-1558; HCoV OC43), which was tested to determine cross-reactivity with SARS-N mAb, was prepared from MRC-5 cells.

Techniques: Immunofluorescence, Infection, Positive Control, Negative Control, Fluorescence, Enzyme-linked Immunosorbent Assay, Control