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Corning Life Sciences
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Corning Life Sciences
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Image Search Results
Journal: PLoS ONE
Article Title: Repeated Small Perturbation Approach Reveals Transcriptomic Steady States
doi: 10.1371/journal.pone.0029241
Figure Lengend Snippet: A ) Three UVB exposures are indicated by UV at 0, 8, and 16 hours. T 1–6 denote the sampling time points. T 1, 3, 5 are timed 30 minutes after the corresponding UVB irradiation. T 2, 4, 6 are timed 8 hours after each UVB irradiation. At each sampling time point, two samples (control and UV-irradiated) were collected. B ) Seventeen dual-color microarray slides, each represented by an arrow, are used to measure the expression levels of the 12 samples. UVi/Ci denotes the UV-irradiated/control sample harvested at time T i . Samples appearing at the tail/head of an arrow are labelled with Cy3/Cy5. Slides no. 14 and 16 are dye-swap hybridizations. Slides no. 11 and 15 are technical replications. Slide no. 17 is a mock experiment.
Article Snippet: A
Techniques: Sampling, Irradiation, Control, Microarray, Expressing
Journal: PLoS ONE
Article Title: Repeated Small Perturbation Approach Reveals Transcriptomic Steady States
doi: 10.1371/journal.pone.0029241
Figure Lengend Snippet: A and B ) Seven genes were chosen randomly from the set of genes with steady-state characteristics for this comparison. egfl5, cnn3, and bcar3 ( A ) exhibit immediate down-regulated responses, while pmaip1, jun, id2, and gadd45a ( B ) exhibit immediate up-regulated responses followed by relaxation. Solid and dash lines represent real-time PCR data and microarray data, respectively. The error bar of the microarray data indicates standard deviation estimated by the log linear model. The error bar of real-time PCR data is the standard deviation derived from three sets of CTs. C ) Scatter plot of real-time PCR results versus microarray data. The correlation coefficient, R is 0.93. The red line is a linear fit through all points. These results clearly demonstrate that even at the small fold change data points, these two sets of data agree very well. It also demonstrates the high reliability and resolution of our cDNA microarray system, which makes this kind of small perturbation study possible.
Article Snippet: A
Techniques: Comparison, Real-time Polymerase Chain Reaction, Microarray, Standard Deviation, Derivative Assay