Journal: EMBO Reports

Article Title: Ubiquitylation of nucleic acids by DELTEX ubiquitin E3 ligase DTX3L

doi: 10.1038/s44319-024-00235-1

Figure Lengend Snippet: Reagents and tools table

Article Snippet: 3′-deoxy ATP , Santa Cruz Biotechnology , sc-396713.

Techniques: Protease Inhibitor, TNKS1 Histone Ribosylation Assay, Recombinant, Ubiquitin Proteomics, Sequencing

Journal: Acta biochimica et biophysica Sinica

Article Title: Cordycepin protects renal ischemia/reperfusion injury through regulating inflammation, apoptosis, and oxidative stress.

doi: 10.1093/abbs/gmz145

Figure Lengend Snippet: Figure 1. Chemical structure of cordycepin

Article Snippet: Cordycepin (sc-203902, purity >98%) was obtained from Santa Cruz Biotechnology (Santa Cruz, USA) and was dissolved in dimethyl sulfoxide (5 mg/ml) as a stock solution.

Techniques:

Journal: Acta biochimica et biophysica Sinica

Article Title: Cordycepin protects renal ischemia/reperfusion injury through regulating inflammation, apoptosis, and oxidative stress.

doi: 10.1093/abbs/gmz145

Figure Lengend Snippet: Figure 2. Treatment with cordycepin attenuates organ injury after renal I/R Cordycepin treatment in rats was conducted by oral gavage for 7 consecutive days (8, 4, and 2 mg/kg, once a day) before the I/R surgery. The renal and blood samples were harvested at 24 h after reperfusion (n = 6). (A) Hematoxylin and eosin staining (H&E) of representative renal sections (magnification 200×). (B) Paller scores for the histological appearance of acute kidney necrosis. (C and D) Circulating levels of BUN and Cr in each experimental group. Data are presented as the mean ± SD. #P < 0.05 or ##P < 0.01 versus sham group, ∗P < 0.05 or ∗∗P < 0.01 versus I/R group.

Article Snippet: Cordycepin (sc-203902, purity >98%) was obtained from Santa Cruz Biotechnology (Santa Cruz, USA) and was dissolved in dimethyl sulfoxide (5 mg/ml) as a stock solution.

Techniques: Staining

Journal: Acta biochimica et biophysica Sinica

Article Title: Cordycepin protects renal ischemia/reperfusion injury through regulating inflammation, apoptosis, and oxidative stress.

doi: 10.1093/abbs/gmz145

Figure Lengend Snippet: Figure 3. Cordycepin reduces the release of pro-inflammatory factors and the expression of inflammation-related pathways Cordycepin treatment in rats was conducted by oral gavage for 7 consecutive days (8, 4, and 2 mg/kg, once a day) before the I/R surgery. The renal and blood samples were harvested at 24 h after reperfusion (n = 6). (A–C) The expression of pro-inflammatory factors (IL-1b, IL-6, TNF-a) in renal tissue were measured by real-time PCR. (D) Detection of MyD88/TLR4 protein expression in renal tissue by western blot analysis reflected the intensity of inflammation laterally (each rat was tested separately). (E and F) Quantitative analyses of the expression of TLR4 and MyD88 proteins by comparing the gray scale value. Data are presented as the mean ± SD. #P < 0.05 or ##P < 0.01 versus sham group; ∗P < 0.05 or ∗∗P < 0.01 versus I/R group.

Article Snippet: Cordycepin (sc-203902, purity >98%) was obtained from Santa Cruz Biotechnology (Santa Cruz, USA) and was dissolved in dimethyl sulfoxide (5 mg/ml) as a stock solution.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Acta biochimica et biophysica Sinica

Article Title: Cordycepin protects renal ischemia/reperfusion injury through regulating inflammation, apoptosis, and oxidative stress.

doi: 10.1093/abbs/gmz145

Figure Lengend Snippet: Figure 4. Cordycepin alleviates apoptosis after I/R Cordycepin treatment in rats was conducted by oral gavage for 7 consecutive days (8, 4, and 2 mg/kg, once a day) before the I/R surgery. The renal and blood samples were harvested at 24 h after reperfusion (n = 6). (A) TUNEL staining of representative renal sections (magnification 400×). (B) Western blot analysis of cleaved caspase-3 and cleaved caspase-9 proteins which can reflect the degree of apoptosis. (C and D) Quantitative analyses of the expression of cleaved caspase-3 and cleaved caspase-9 proteins by comparing the gray scale value. Data are presented as the mean ± SD. #P < 0.05 or ##P < 0.01 versus sham group; ∗P < 0.05 or ∗∗P < 0.01 versus I/R group.

Article Snippet: Cordycepin (sc-203902, purity >98%) was obtained from Santa Cruz Biotechnology (Santa Cruz, USA) and was dissolved in dimethyl sulfoxide (5 mg/ml) as a stock solution.

Techniques: TUNEL Assay, Staining, Western Blot, Expressing

Journal: Acta biochimica et biophysica Sinica

Article Title: Cordycepin protects renal ischemia/reperfusion injury through regulating inflammation, apoptosis, and oxidative stress.

doi: 10.1093/abbs/gmz145

Figure Lengend Snippet: Figure 5. Cordycepin attenuates oxidative stress after renal I/R Cordycepin treatment in rats was conducted by oral gavage for 7 consecutive days (8, 4, and 2 mg/kg, once a day) before the I/R surgery. The renal and blood samples were harvested at 24 h after reperfusion (n = 6). The levels of (A) MDA, (B) GSH, (C) GSH-Px, (D) SOD, (E) NO, and (F) iNOS were detected in I/R kidney tissues. Data are presented as the mean ± SD. #P < 0.05 or ##P < 0.01 versus sham group; ∗P < 0.05, ∗∗P < 0.01 versus I/R group.

Article Snippet: Cordycepin (sc-203902, purity >98%) was obtained from Santa Cruz Biotechnology (Santa Cruz, USA) and was dissolved in dimethyl sulfoxide (5 mg/ml) as a stock solution.

Techniques:

Journal: Acta biochimica et biophysica Sinica

Article Title: Cordycepin protects renal ischemia/reperfusion injury through regulating inflammation, apoptosis, and oxidative stress.

doi: 10.1093/abbs/gmz145

Figure Lengend Snippet: Figure 6. Cordycepin reduces ROS production and expressions of oxidative stress-related proteins Cordycepin treatment in rats was conducted by oral gavage for 7 consecutive days (8, 4, and 2 mg/kg, once a day) before the I/R surgery. The renal and blood samples were harvested at 24 h after reperfusion (n = 6). (A) The production of ROS in each group after I/R (magnification 400×). (B) Expression of Nrf2 and HO-1 which represent major antioxidant stress proteins was measured by western blot analysis. (C and D) Quantitative analyses of the expression of Nrf2 and HO-1 proteins by comparing the gray scale value. Data are presented as the mean ± SD. #P < 0.05 or ##P < 0.01 versus sham group, ∗P < 0.05 or ∗∗P < 0.01 versus I/R group.

Article Snippet: Cordycepin (sc-203902, purity >98%) was obtained from Santa Cruz Biotechnology (Santa Cruz, USA) and was dissolved in dimethyl sulfoxide (5 mg/ml) as a stock solution.

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: A novel nucleoside rescue metabolic pathway may be responsible for therapeutic effect of orally administered cordycepin

doi: 10.1038/s41598-019-52254-x

Figure Lengend Snippet: Multiple reaction monitoring parameters of the LC-MS/MS conditions of cordycepin, 3′-deoxyinosine and CordyTP.

Article Snippet: Cordycepin (3′-deoxyadenosine, CAS: 73-03-0) was obtained from Carbosynth Ltd (Berkshire, UK).

Techniques: Targeted Proteomics

Journal: Scientific Reports

Article Title: A novel nucleoside rescue metabolic pathway may be responsible for therapeutic effect of orally administered cordycepin

doi: 10.1038/s41598-019-52254-x

Figure Lengend Snippet: In vitro biopharmaceutical assessment of cordycepin (mean ± SD). ( a ) Time-dependent degradation of cordycepin in rat and human plasma (n = 4). ( b ) Stabilisation effect of pentostatin on cordycepin tested in rat plasma at different concentrations of pentostatin (1 pM – 10 µM) (n = 4). **p < 0.01 compared to control group (without pentostatin). ( c ) Plasma protein binding results of cordycepin in rat and human plasma presented as fraction unbound ( F ub ) in plasma (n = 4). ( d ) Biorelevant solubility of cordycepin tested in fasted state simulated gastric fluid (FaSSGF), fasted state simulated intestinal fluid (FaSSIF) and fed state simulated intestinal fluid (FeSSIF) (n = 4). **p < 0.01. ( e ) Permeability of cordycepin tested using Caco-2 cells with co-administration of pentostatin at both apical-to-basolateral (A to B) and basolateral-to-apical (B to A) directions (n = 3). (f) Permeation of 3′-deoxyinosine when Caco-2 cells are treated with cordycepin without co-administration of pentostatin. In this case, permeation of cordycepin was not detected (n = 3). ( g ) Cordycepin concentrations analysed before and after the permeability experiment in the donor chambers (n = 3). **p < 0.01.

Article Snippet: Cordycepin (3′-deoxyadenosine, CAS: 73-03-0) was obtained from Carbosynth Ltd (Berkshire, UK).

Techniques: In Vitro, Clinical Proteomics, Control, Protein Binding, Solubility, Permeability

Journal: Scientific Reports

Article Title: A novel nucleoside rescue metabolic pathway may be responsible for therapeutic effect of orally administered cordycepin

doi: 10.1038/s41598-019-52254-x

Figure Lengend Snippet: Liver microsomal stability of cordycepin tested with rat and human liver microsomes (mean ± SD, n = 3).

Article Snippet: Cordycepin (3′-deoxyadenosine, CAS: 73-03-0) was obtained from Carbosynth Ltd (Berkshire, UK).

Techniques:

Journal: Scientific Reports

Article Title: A novel nucleoside rescue metabolic pathway may be responsible for therapeutic effect of orally administered cordycepin

doi: 10.1038/s41598-019-52254-x

Figure Lengend Snippet: In vivo pharmacokinetic profiles of cordycepin and its metabolite, 3′-deoxyinosine, following administration of cordycepin in rats (mean ± SD). ( a ) Profiles of cordycepin following intravenous administration of cordycepin at 8 or 20 mg/kg (n = 4). ( b ) Profiles of 3′-deoxyinosine following intravenous administration of cordycepin at 8 or 20 mg/kg (n = 4). ( c ) Profiles of 3′-deoxyinosine following oral administration of cordycepin at 8 or 80 mg/kg (n = 5).

Article Snippet: Cordycepin (3′-deoxyadenosine, CAS: 73-03-0) was obtained from Carbosynth Ltd (Berkshire, UK).

Techniques: In Vivo

Journal: Scientific Reports

Article Title: A novel nucleoside rescue metabolic pathway may be responsible for therapeutic effect of orally administered cordycepin

doi: 10.1038/s41598-019-52254-x

Figure Lengend Snippet: Pharmacokinetic parameters of cordycepin following intravenous administration of cordycepin.

Article Snippet: Cordycepin (3′-deoxyadenosine, CAS: 73-03-0) was obtained from Carbosynth Ltd (Berkshire, UK).

Techniques:

Journal: Scientific Reports

Article Title: A novel nucleoside rescue metabolic pathway may be responsible for therapeutic effect of orally administered cordycepin

doi: 10.1038/s41598-019-52254-x

Figure Lengend Snippet: Pharmacokinetic parameters of 3′-deoxyinosine following intravenous and oral administration of cordycepin.

Article Snippet: Cordycepin (3′-deoxyadenosine, CAS: 73-03-0) was obtained from Carbosynth Ltd (Berkshire, UK).

Techniques:

Journal: Scientific Reports

Article Title: A novel nucleoside rescue metabolic pathway may be responsible for therapeutic effect of orally administered cordycepin

doi: 10.1038/s41598-019-52254-x

Figure Lengend Snippet: Repression effect on inflammatory gene expression. ( a ) NBTI or ITu treatment prior to cordycepin treatment suppresses effect of cordycepin. RAW264.7 cells were treated with 10 µM NBTI, 100 nM ITu or DMSO (Ctrl, control) 15 min prior to cordycepin treatment. The cells were stimulated with LPS 1 h after cordycepin treatment (mean ± SD, n = 3). **p < 0.01; ***p < 0.001 compared to DMSO treatment (labelled as Ctrl). ( b ) 3′-deoxyinosine exerts same repression effect as cordycepin, although to a lesser degree. RAW264.7 cells were treated with 20 µM cordycepin, 20 µM 3′-deoxyinosine (3DI) or DMSO (Ctrl, control) for 1 h prior to LPS stimulation (mean ± SD, n = 3). **p < 0.01; ***p < 0.001 compared to without compound treatment (labelled as LPS).

Article Snippet: Cordycepin (3′-deoxyadenosine, CAS: 73-03-0) was obtained from Carbosynth Ltd (Berkshire, UK).

Techniques: Gene Expression, Control

Journal: Scientific Reports

Article Title: A novel nucleoside rescue metabolic pathway may be responsible for therapeutic effect of orally administered cordycepin

doi: 10.1038/s41598-019-52254-x

Figure Lengend Snippet: Levels of cordycepin, 3′-deoxyinosine and CordyTP found following in vitro treatment with cordycepin or 3′-deoxyinosine. RAW264.7 cells maintained in 0.5 or 10% (foetal bovine serum (FBS) conditions were treated with cordycepin or 3′-deoxyinosine at 20 µM for 6 h (mean ± SD, n = 3).

Article Snippet: Cordycepin (3′-deoxyadenosine, CAS: 73-03-0) was obtained from Carbosynth Ltd (Berkshire, UK).

Techniques: In Vitro

Journal: Scientific Reports

Article Title: A novel nucleoside rescue metabolic pathway may be responsible for therapeutic effect of orally administered cordycepin

doi: 10.1038/s41598-019-52254-x

Figure Lengend Snippet: Proposed metabolic pathways of cordycepin. The thicker arrows indicate the novel nucleoside rescue metabolic pathway proposed in this study.

Article Snippet: Cordycepin (3′-deoxyadenosine, CAS: 73-03-0) was obtained from Carbosynth Ltd (Berkshire, UK).

Techniques:

Journal: Acta Cirúrgica Brasileira

Article Title: Cordycepin prevents the esophageal stricture formation in the alkali-burn rat model by exerting anti-inflammatory and antifibrotic effects

doi: 10.1590/ACB360302

Figure Lengend Snippet: Histological images of esophageal tissues of the rats in sham group (a) , control group (b) , cordycepin group (c) and prednisolone group (d) . Magnifications × 4, Masson trichrome.

Article Snippet: Cordycepin group : rats in this group underwent a laparotomy with establishment of esophageal alkali-burn, then treated with a daily dose of 20 mg/kg cordycepin (Toronto Research Chemicals Inc, Canada) injected intraperitoneally for 28 days.

Techniques: Control

Journal: Acta Cirúrgica Brasileira

Article Title: Cordycepin prevents the esophageal stricture formation in the alkali-burn rat model by exerting anti-inflammatory and antifibrotic effects

doi: 10.1590/ACB360302

Figure Lengend Snippet: The HDS of esophageal tissues of rats.

Article Snippet: Cordycepin group : rats in this group underwent a laparotomy with establishment of esophageal alkali-burn, then treated with a daily dose of 20 mg/kg cordycepin (Toronto Research Chemicals Inc, Canada) injected intraperitoneally for 28 days.

Techniques: Control

Journal: Acta Cirúrgica Brasileira

Article Title: Cordycepin prevents the esophageal stricture formation in the alkali-burn rat model by exerting anti-inflammatory and antifibrotic effects

doi: 10.1590/ACB360302

Figure Lengend Snippet: The SI of esophageal tissues of rats.

Article Snippet: Cordycepin group : rats in this group underwent a laparotomy with establishment of esophageal alkali-burn, then treated with a daily dose of 20 mg/kg cordycepin (Toronto Research Chemicals Inc, Canada) injected intraperitoneally for 28 days.

Techniques: Control

Journal: Acta Cirúrgica Brasileira

Article Title: Cordycepin prevents the esophageal stricture formation in the alkali-burn rat model by exerting anti-inflammatory and antifibrotic effects

doi: 10.1590/ACB360302

Figure Lengend Snippet: H-scores of the immunohistochemical analysis of esophageal tissues of rats.

Article Snippet: Cordycepin group : rats in this group underwent a laparotomy with establishment of esophageal alkali-burn, then treated with a daily dose of 20 mg/kg cordycepin (Toronto Research Chemicals Inc, Canada) injected intraperitoneally for 28 days.

Techniques: Immunohistochemical staining, Control

Journal: Acta Cirúrgica Brasileira

Article Title: Cordycepin prevents the esophageal stricture formation in the alkali-burn rat model by exerting anti-inflammatory and antifibrotic effects

doi: 10.1590/ACB360302

Figure Lengend Snippet: The immunohistochemical analysis of HIF-1α in sham group, × ٢٠ ( a ), control group, × ٤٠ ( b ), cordycepin group, × 20 ( c ) and prednisolone group, × 40 ( d ).

Article Snippet: Cordycepin group : rats in this group underwent a laparotomy with establishment of esophageal alkali-burn, then treated with a daily dose of 20 mg/kg cordycepin (Toronto Research Chemicals Inc, Canada) injected intraperitoneally for 28 days.

Techniques: Immunohistochemical staining, Control

Journal: Acta Cirúrgica Brasileira

Article Title: Cordycepin prevents the esophageal stricture formation in the alkali-burn rat model by exerting anti-inflammatory and antifibrotic effects

doi: 10.1590/ACB360302

Figure Lengend Snippet: The immunohistochemical analysis of TNFα in sham group × 20 ( a ), control group, × 40 ( b ), cordycepin group, × 20 ( c ) and prednisolone group, × 20 ( d ).

Article Snippet: Cordycepin group : rats in this group underwent a laparotomy with establishment of esophageal alkali-burn, then treated with a daily dose of 20 mg/kg cordycepin (Toronto Research Chemicals Inc, Canada) injected intraperitoneally for 28 days.

Techniques: Immunohistochemical staining, Control

Journal: Acta Cirúrgica Brasileira

Article Title: Cordycepin prevents the esophageal stricture formation in the alkali-burn rat model by exerting anti-inflammatory and antifibrotic effects

doi: 10.1590/ACB360302

Figure Lengend Snippet: The immunohistochemical analysis of VEGFA in sham group, × 20 (a) , control group, × 20 (b) , cordycepin group, × 20 (c) and prednisolone group, × 20 (d) .

Article Snippet: Cordycepin group : rats in this group underwent a laparotomy with establishment of esophageal alkali-burn, then treated with a daily dose of 20 mg/kg cordycepin (Toronto Research Chemicals Inc, Canada) injected intraperitoneally for 28 days.

Techniques: Immunohistochemical staining, Control

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Stress induces corticosterone-mediated CD8 + T cell exhaustion to promote non-small cell lung cancer

doi: 10.1007/s00262-025-04179-w

Figure Lengend Snippet: Stress elevates corticosterone in blood and glucocorticoid receptor (GR) in tumor. The concentration of a adrenocorticotropic hormone (ACTH), b corticosterone (COR), c adrenaline and d norepinephrine were tested by ELISA at the tumor endpoint between the control (Con) and acute restraint (AR) mice (n = 6). (e-f) Correlation analysis between ACTH and COR with LDBT and OFT, respectively. (g-j) Correlation analysis between tumor volumes with concentration of ACTH, COR, norepinephrine and adrenaline, respectively. k Immunofluorescence analysis of the expression levels of GR and CD8a in tumor tissues and the white arrows point to CD8 + T cells with high expression of GR (50x). l Flow cytometric analysis of the expression of GR in CD8 + T cells of the control (Con) and corticosterone treatment (COR) mice (n = 3). Data are represented as the mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 by an unpaired Student’s t-test or Pearson analysis

Article Snippet: What is more, mice of COR exposure group were given water containing 1.3μg/ml corticosterone (S2559, Selleck) which was diluted in 0.002% DMSO and balanced with 0.02% 2-hydroxypropyl-β-cyclodextrin as vehicle, according to Hong et al. [ ].

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control, Immunofluorescence, Expressing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Stress induces corticosterone-mediated CD8 + T cell exhaustion to promote non-small cell lung cancer

doi: 10.1007/s00262-025-04179-w

Figure Lengend Snippet: Corticosterone (COR) promotes CD8 + T cell exhaustion and NSCLC growth. a The LLC tumor growth curves and the endpoint tumor weights were displayed of control (Con) and corticosterone treatment (COR) mice (n = 5). (b-d) Flow cytometric analysis of the proportions of PD-1 + and LAG3 + , granzyme B + and IFN-γ + of CD8 + T cells of tumor tissues from Con and COR mice (n = 4). (e-f) The LLC tumor growth curves, the endpoint tumor weights and tumor sizes were showed of acute restraint (AR+PBS) and acute restraint+RU486 treatment (AR+RU486) mice (n = 6). g CCK-8 detection of CD8 + T cell viability after 24 hours of treatment with different concentrations of COR. Flow cytometric analysis of the proportions of h IFN-γ + , i granzyme B + , j LAG3 + , k PD-1 + and l TIM-3 + of CD8 + T cells among DMSO, COR and COR+RU486 treatment groups (n = 3). Data are represented as the mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by an unpaired Student’s T-test or one-way ANOVA

Article Snippet: What is more, mice of COR exposure group were given water containing 1.3μg/ml corticosterone (S2559, Selleck) which was diluted in 0.002% DMSO and balanced with 0.02% 2-hydroxypropyl-β-cyclodextrin as vehicle, according to Hong et al. [ ].

Techniques: Control, CCK-8 Assay