Journal: Virus Genes

Article Title: Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines

doi: 10.1007/s11262-012-0713-1

Figure Lengend Snippet: Published cases of PEDV outbreaks from several countries (1978–2011)

Article Snippet: Especially, endemic situation of PED infection brought the several commercialised PED virus detection systems using diagnosis techniques including conventional duplex RT-PCR (iNtRON Biotechnology, Inc, Korea), real time RT-PCR (kogenebiotech, Kore), DPO based multiplex RT-PCR (Seegene, Seoul, Korea), and immunochromatography (Bionote, Korea) in Korea.

Techniques: Virus, Infection, Isolation, Histopathology, Neutralization

Journal: Biomedicines

Article Title: Exploring the Role of Guanylate-Binding Protein-2 in Activated Microglia-Mediated Neuroinflammation and Neuronal Damage

doi: 10.3390/biomedicines12051130

Figure Lengend Snippet: The induction for GBP2 expression in the presence of various conditions. ( A , B ) GBP2 expression according to the treatment of LPS concentration. BV2 microglial cells were incubated with various concentrations of LPS for 2 h. GBP2 expression was confirmed by conventional PCR. The increased induction of GBP2 expression was observed when treated with 1000 ng/mL LPS. ( C , D ) GBP2 expression according to the time of LPS treatment. BV2 microglial cells treated with 1000 ng/mL LPS were incubated in a time-dependent manner, and then confirmed by conventional PCR at each time. GBP2 expression increased most significantly at 4 h. Quantifications of PCR band intensities for GBP2 expression were normalized to their respective controls (defined as 1.0). Data shown represent the mean ± SEM ( n = 3). *** p < 0.01 for the comparison with the control group.

Article Snippet: Then, conventional PCR (Solgent) was performed in accordance with the conditions of 95 °C for 3 min, 95 °C for 30 s, 72 °C for 30 s, 72 °C for 5 min, and 4 °C (∞), and 2% agarose were made, and then the target gene of GBP2 was identified.

Techniques: Expressing, Concentration Assay, Incubation, Comparison, Control

Journal: Journal of Clinical Microbiology

Article Title: Optimization and Evaluation of a Multiplex Quantitative PCR Assay for Detection of Nucleic Acids in Human Blood Samples from Patients with Spotted Fever Rickettsiosis, Typhus Rickettsiosis, Scrub Typhus, Monocytic Ehrlichiosis, and Granulocytic Anaplasmosis

doi: 10.1128/JCM.01802-19

Figure Lengend Snippet: Specimens used for evaluation of multiplex PCR

Article Snippet: Convalescent from E. chaffeensis or A. phagocytophilum (3) , Johns Hopkins University School of Medicine (USA) , Blood , Conventional PCR (3), serology (3), morulae (2), culture (3) , 26.

Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction