conventional amplification Search Results


gene exp pcsk1n hs00560041 m1  (Thermo Fisher)
91
Thermo Fisher gene exp pcsk1n hs00560041 m1
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Gene Exp Pcsk1n Hs00560041 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/Gene+Exp%2E+PCSK1N%2C+Hs00560041_m1/bio_rxiv__2022__11__20__517219-62-27-23
Average 91 stars, based on 1 article reviews
gene exp pcsk1n hs00560041 m1 - by Bioz Stars, 2026-10
91/100 stars
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bisulfite converted dna  (Thermo Fisher)
99
Thermo Fisher bisulfite converted dna
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Bisulfite Converted Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/DNA/pmc08665281-427-40-45
Average 99 stars, based on 1 article reviews
bisulfite converted dna - by Bioz Stars, 2026-10
99/100 stars
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loopamp rna amplification kit  (Eiken Chemical)
90
Eiken Chemical loopamp rna amplification kit
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Loopamp Rna Amplification Kit, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/loopamp+rna+amplification+kit/pm17685931-45-36-40
Average 90 stars, based on 1 article reviews
loopamp rna amplification kit - by Bioz Stars, 2026-10
90/100 stars
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a conventional patch-clamp amplifier  (HEKA Elektronik)
90
HEKA Elektronik a conventional patch-clamp amplifier
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
A Conventional Patch Clamp Amplifier, supplied by HEKA Elektronik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/epc+9+patch+clamp+amplifier/pm37001827-99-2-7
Average 90 stars, based on 1 article reviews
a conventional patch-clamp amplifier - by Bioz Stars, 2026-10
90/100 stars
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digidata 1322 a d converter  (Molecular Devices LLC)
99
Molecular Devices LLC digidata 1322 a d converter
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Digidata 1322 A D Converter, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/MultiClamp+Amplifier/pmc03528716-72-13-29
Average 99 stars, based on 1 article reviews
digidata 1322 a d converter - by Bioz Stars, 2026-10
99/100 stars
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idac a/d converter  (Syntech GmbH)
90
Syntech GmbH idac a/d converter
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Idac A/D Converter, supplied by Syntech GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/idac+analog+digital+converter/pmc09147048-99-14-21
Average 90 stars, based on 1 article reviews
idac a/d converter - by Bioz Stars, 2026-10
90/100 stars
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high-input impedance dc amplifier interface idac-2  (Syntech GmbH)
90
Syntech GmbH high-input impedance dc amplifier interface idac-2
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
High Input Impedance Dc Amplifier Interface Idac 2, supplied by Syntech GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/idac+2/pmc07469176-127-14-20
Average 90 stars, based on 1 article reviews
high-input impedance dc amplifier interface idac-2 - by Bioz Stars, 2026-10
90/100 stars
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low noise amplifier  (Mini-Circuits)
93
Mini-Circuits low noise amplifier
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Low Noise Amplifier, supplied by Mini-Circuits, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/Plug-In+Low+Noise+Linear+Amplifier/pm37633311-303-8-10
Average 93 stars, based on 1 article reviews
low noise amplifier - by Bioz Stars, 2026-10
93/100 stars
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multi-functional generator ag1020  (Power Conversion Inc)
90
Power Conversion Inc multi-functional generator ag1020
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Multi Functional Generator Ag1020, supplied by Power Conversion Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/multi+functional+generator+ag1020/pm24752138-39-9-13
Average 90 stars, based on 1 article reviews
multi-functional generator ag1020 - by Bioz Stars, 2026-10
90/100 stars
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gene exp ace mm00802048 m1  (Thermo Fisher)
97
Thermo Fisher gene exp ace mm00802048 m1
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Gene Exp Ace Mm00802048 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/Gene+Exp%2E+Ace%2C+Mm00802048_m1/pmc04510986-244-33-9
Average 97 stars, based on 1 article reviews
gene exp ace mm00802048 m1 - by Bioz Stars, 2026-10
97/100 stars
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chi(3)-based phase-sensitive amplifier or frequency converter  (CH Instruments)
90
CH Instruments chi(3)-based phase-sensitive amplifier or frequency converter
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Chi(3) Based Phase Sensitive Amplifier Or Frequency Converter, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/iodoform++chi+3+/us10050720-83-35-31
Average 90 stars, based on 1 article reviews
chi(3)-based phase-sensitive amplifier or frequency converter - by Bioz Stars, 2026-10
90/100 stars
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pcmv hace2 plasmid  (Sino Biological)
96
Sino Biological pcmv hace2 plasmid
Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) <t>KISS1</t> and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.
Pcmv Hace2 Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conventional+amplification/Human+ACE2+%2F+Angiotensin-Converting+Enzyme+2+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-HA+tag/pmc09438359-214-7-9
Average 96 stars, based on 1 article reviews
pcmv hace2 plasmid - by Bioz Stars, 2026-10
96/100 stars
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Image Search Results


Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) KISS1 and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.

Journal: bioRxiv

Article Title: Human endometrial KISS1R inhibits stromal cell decidualization in a manner associated with a reduction in ESR1 levels

doi: 10.1101/2022.11.20.517219

Figure Lengend Snippet: Quantification of mRNA levels of hallmark molecular markers of decidualization (A) FOXO1 , (B) IGFBP1 , (C) PRL and ( D ) representative western blot analysis of endometrial FOXO1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization (see Fig. S2 for additional blots). Quantification of mRNA levels of known regulators of ESC decidualization ( E ) DCN , ( F ) LEFTY2 , and ( G ) WNT4 before and after six days of decidualization. qPCR analysis of mRNA expression of ( H ) KISS1 and ( I and J ) KISS1R before and after one, two, four, and six days of decidualization. Statistics (biological replicates: n=3-6 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test and one-way ANOVA with Bonferroni Multiple Comparison post hoc test. * p<0.05; ** p<0.01; and *** p<0.001. D0: day zero; D1: day one; D2: day two; D4: day four; D6: day six of decidualization; BM: basal medium; and EPC: decidualization medium.

Article Snippet: The amplified cDNA was diluted to 10 ng/μl, and quantitative PCR was performed using 40 ng of cDNA with the following TaqMan probes (Applied Biosystems/Life Technologies), KISS1 (Hs00560041_m1), and KISS1R (Hs00173906_m1).

Techniques: Western Blot, Expressing, Two Tailed Test

Quantification of mRNA and protein levels in empty vector control and KISS1R expressing primary HESCs following six days of in vitro decidualization. qPCR analysis of ( A) KISS1 , ( B ) KISS1R and phenotypic markers of decidualization ( C ) DESMIN, KRT7 , and KRT18 mRNA levels. Representative images of the immunofluorescence analysis of cytokeratin 7 protein levels in empty vector control ( D1-3 ) and KISS1R ( D4-6 ) expressing primary HESCs. ( E ) qPCR analysis of decidualization and ESR1 signaling markers FOXO1, IGFBP1, PRL, DCN, LEFTY2, WNT4, ESR1, PGR, MMP2 , and MMP7 mRNA levels. Representative western blot analysis of endometrial FOXO1 and ESR1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization in empty vector and KISS1R expressing HESCs (see Fig. S3 for additional blots). Statistics (biological replicates: n=3-5 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test. * p<0.05; ** p<0.01; and *** p<0.001. EV: Empty vector control; KISS1R or KR: KISS1R expression; BM: basal medium; and EPC: decidualization medium.

Journal: bioRxiv

Article Title: Human endometrial KISS1R inhibits stromal cell decidualization in a manner associated with a reduction in ESR1 levels

doi: 10.1101/2022.11.20.517219

Figure Lengend Snippet: Quantification of mRNA and protein levels in empty vector control and KISS1R expressing primary HESCs following six days of in vitro decidualization. qPCR analysis of ( A) KISS1 , ( B ) KISS1R and phenotypic markers of decidualization ( C ) DESMIN, KRT7 , and KRT18 mRNA levels. Representative images of the immunofluorescence analysis of cytokeratin 7 protein levels in empty vector control ( D1-3 ) and KISS1R ( D4-6 ) expressing primary HESCs. ( E ) qPCR analysis of decidualization and ESR1 signaling markers FOXO1, IGFBP1, PRL, DCN, LEFTY2, WNT4, ESR1, PGR, MMP2 , and MMP7 mRNA levels. Representative western blot analysis of endometrial FOXO1 and ESR1 protein levels with tubulin serving as the housekeeping control before and after six days of decidualization in empty vector and KISS1R expressing HESCs (see Fig. S3 for additional blots). Statistics (biological replicates: n=3-5 biopsies and technical replicates: n=3-6 for each group): student’s unpaired two-tailed t-test. * p<0.05; ** p<0.01; and *** p<0.001. EV: Empty vector control; KISS1R or KR: KISS1R expression; BM: basal medium; and EPC: decidualization medium.

Article Snippet: The amplified cDNA was diluted to 10 ng/μl, and quantitative PCR was performed using 40 ng of cDNA with the following TaqMan probes (Applied Biosystems/Life Technologies), KISS1 (Hs00560041_m1), and KISS1R (Hs00173906_m1).

Techniques: Plasmid Preparation, Expressing, In Vitro, Immunofluorescence, Western Blot, Two Tailed Test