Journal: iScience

Article Title: Migration of human T cells can be differentially directed by electric fields depending on the extracellular microenvironment

doi: 10.1016/j.isci.2024.109746

Figure Lengend Snippet:

Article Snippet: Mouse IgG2a isotype control - FITC , Miltenyi Biotec , Cat#: 130-113-271 Lot: 5200908299.

Techniques: Recombinant, Control, Cell Isolation, Isolation, Software

Journal: Cells

Article Title: TLR7 Expression Is Associated with M2 Macrophage Subset in Calcific Aortic Valve Stenosis

doi: 10.3390/cells9071710

Figure Lengend Snippet: TLR7 correlates with M2 macrophage markers in healthy, intermediate, and calcified parts of the aortic valve. ( a ) TLR7 mRNA levels were significantly increased in calcified compared with healthy and intermediate parts of the aortic valve ( n = 55). 1-way RM ANOVA with Holm-Sidak post-hoc test, **** p ≤ 0.0001. ( b – d ) Correlation analysis of TLR7 mRNA levels with mRNA levels of the VIC marker gene vimentin as well as immune cell markers in healthy ( b ), intermediate ( c ), and calcified ( d ) parts of human aortic valves ( n = 55). Pearson correlation and false discovery rate (FDR) adjusted p -values are presented.

Article Snippet: The TLR7 agonist imiquimod (IMQ, Cat. #tlrl-imq) was purchased from Invivogen (Toulouse, France), and the TLR7 inhibitor ODN20958 (Cat. #130-105-820) was purchased from Miltenyi (Bergisch Gladbach, Germany).

Techniques: Marker

Journal: Cells

Article Title: TLR7 Expression Is Associated with M2 Macrophage Subset in Calcific Aortic Valve Stenosis

doi: 10.3390/cells9071710

Figure Lengend Snippet: Gene expression of TLR7 in healthy, intermediate, and calcified parts of the aortic valve.

Article Snippet: The TLR7 agonist imiquimod (IMQ, Cat. #tlrl-imq) was purchased from Invivogen (Toulouse, France), and the TLR7 inhibitor ODN20958 (Cat. #130-105-820) was purchased from Miltenyi (Bergisch Gladbach, Germany).

Techniques: Gene Expression

Journal: Cells

Article Title: TLR7 Expression Is Associated with M2 Macrophage Subset in Calcific Aortic Valve Stenosis

doi: 10.3390/cells9071710

Figure Lengend Snippet: Co-localization of TLR7 and macrophage markers in human aortic valves, visualized by immunofluorescence staining. ( a ) TLR7 (red) is expressed in CD68 + (green) cells. ( b ) double staining of TLR7 (red) and CD206 (green). ( c ) co-localization of TLR7 (red) and CD163 (green). ( d ) TLR7 (red) is expressed in CD3 + (green) T cells. Nuclei are stained blue. Scale bars: 20 µm.

Article Snippet: The TLR7 agonist imiquimod (IMQ, Cat. #tlrl-imq) was purchased from Invivogen (Toulouse, France), and the TLR7 inhibitor ODN20958 (Cat. #130-105-820) was purchased from Miltenyi (Bergisch Gladbach, Germany).

Techniques: Immunofluorescence, Staining, Double Staining

Journal: Cells

Article Title: TLR7 Expression Is Associated with M2 Macrophage Subset in Calcific Aortic Valve Stenosis

doi: 10.3390/cells9071710

Figure Lengend Snippet: Ex vivo stimulation of human aortic valve tissue with the TLR7 agonist IMQ alters cytokine secretion. Human stenotic aortic valve tissue was stimulated ex vivo with the TLR7 ligand imiquimod (IMQ; 12.5 µg/mL). Levels of ( a ) IL-10, ( b ) TNF-α, and ( c ) GMCSF were significantly increased in the culture medium after 20 h. The effects of IMQ were significantly attenuated by the TLR7 antagonist ODN 20958 (5 µM). Data are presented as mean ± SEM. 1-way RM ANOVA with Holm-Sidak post-hoc test, * p < 0.05.

Article Snippet: The TLR7 agonist imiquimod (IMQ, Cat. #tlrl-imq) was purchased from Invivogen (Toulouse, France), and the TLR7 inhibitor ODN20958 (Cat. #130-105-820) was purchased from Miltenyi (Bergisch Gladbach, Germany).

Techniques: Ex Vivo

Journal: Cells

Article Title: TLR7 Expression Is Associated with M2 Macrophage Subset in Calcific Aortic Valve Stenosis

doi: 10.3390/cells9071710

Figure Lengend Snippet: Cytokine response from valvular interstitial cells (VICs) and primary macrophages upon in vitro stimulation with TLR7 ligand. VICs were isolated from four aortic valves derived from patients with aortic valve stenosis. Primary human macrophages were derived from monocytes isolated from blood from four healthy donors. IL-10, TNF-α, and IL-8 were measured in the culture medium after 20-h stimulation of VICs and primary macrophages, respectively, with 12.5 µg/mL IMQ. LPS was used as a positive control for VICs. ( a ) No significant change in IL-10 secretion was observed in VICs after IMQ stimulation. ( b ) TNF-α was below the detection level in VICs supernatant. ( c ) Significant increase of IL-8 upon stimulation with IMQ and LPS, respectively. 1-way ANOVA with Holm-Sidak post-hoc test, ** p < 0.01. ( d ) Increased IL-10 secretion by primary macrophages upon TLR7 activation. ( e ) No significant change in TNF-α secretion was observed in primary macrophages after IMQ stimulation. ( f ) Significant increase of IL-8 upon stimulation of primary macrophages with IMQ. Student´s paired t-test, * p < 0.05, *** p < 0.001. Data presented as mean ± SEM.

Article Snippet: The TLR7 agonist imiquimod (IMQ, Cat. #tlrl-imq) was purchased from Invivogen (Toulouse, France), and the TLR7 inhibitor ODN20958 (Cat. #130-105-820) was purchased from Miltenyi (Bergisch Gladbach, Germany).

Techniques: In Vitro, Isolation, Derivative Assay, Positive Control, Activation Assay