Journal: bioRxiv

Article Title: Circuit directionality for motivation: lateral accumbens-pallidum, but not pallidum-accumbens, connections regulate motivational attraction to reward cues

doi: 10.1101/474387

Figure Lengend Snippet: Each coronal section represents a range of three sections relative to Bregma (B, in mm). Expression for each animal is plotted at 90% transparency and then per-animal expression is overlaid. Sections run anterior (top) to posterior (bottom). A) Expression map of AAV-hSyn-DIO-hM4D(Gi)-mCherry in VP following CAV2-Cre injections in NAcLSh (i.e., VP → NAcLSh inhibition animals; red). B) Expression map of AAV-hSyn-DIO-mCherry in VP (i.e., controls; black).

Article Snippet: The inhibition group received 0.6 µl of AAV-hSyn-DIO-hM4D(Gi)-mCherry (n = 12, AAV5, UNC Vector Core; n = 4, AAV8, Addgene) in the VP and 1.0 µl CAV-Cre or CAV-Cre-GFP in the NAcLSh.

Techniques: Expressing, Inhibition

Journal: Antiviral research

Article Title: LAG3 + erythroid progenitor cells inhibit HBsAg seroclearance during finite pegylated interferon treatment through LAG3 and TGF-β.

doi: 10.1016/j.antiviral.2023.105592

Figure Lengend Snippet: Fig. 4. Accumulation of CD45þEPCs in AAV/HBV mice model. (A) AAV/HBV mouse model was established via tail vein injection of 2.5 × 1011 AAV8-HBV1.3 viral genome (genotype C, serotype adr, pUF1-HBV-C-ADR 1.3), and used AAV8 [ssAAV.CAG.WPRE.SV40pA] as vector control. After four weeks, the mice were sacrificed. Typical flow cytometer gating and statistical analysis of CD45+EPCs and CD45−EPCs in spleen, liver, PBMCs and bone marrow (BM) of vector control (Vector) (n = 6) and AAV/HBV mice model (AAV/HBV) (n = 6). (B) Immunofluorescence staining of CD45+Ter119+CD71+ cells in formalin-fixed liver tissue and statistical analysis of the amounts of CD45+Ter119+CD71+ cells in vector control (Vector) (n = 6) and AAV/HBV mice model (AAV/HBV) (n = 6). CD45+Ter119+CD71+ erythroid cells were stained with anti-CD45 with donkey anti-rabbit Alexa Fluor 594 (red), anti-Ter119 with donkey anti-rat Alexa Fluor 488 (green), anti-CD71 with donkey anti-mouse Alexa Fluor 647 (violet) and DAPI (blue). (C) Immunofluorescence staining of CD45+Ter119+CD71+ cells in formalin- fixed spleen tissue and statistical analysis of the amounts of CD45+Ter119+CD71+ cells in vector control (Vector) (n = 6) and AAV/HBV mice model (n = 6). CD45+Ter119+CD71+ erythroid cells were stained with anti-CD45 with donkey anti-rabbit Alexa Fluor 594 (red), anti-Ter119 with donkey anti-rat Alexa Fluor 488 (green), anti-CD71 with donkey anti-mouse Alexa Fluor 647 (violet) and DAPI (blue). (D) Wright-Giemsa’ stain chart and statistical analysis of the proportion of polychromatic erythroblasts, metarubricytes, erythrocytes and atypical cells in CD45+EPCs and CD45−EPCs of spleen, liver, PBMC and bone marrow in vector control (Vector) (n = 3) and AAV/HBV mice model (AAV/HBV) (n = 3). *, p < 0.05; **, p < 0.01 by Mann-Whitney test (A.B.C) and one-way ANOVA test (D); Abbreviation: EPCs, erythroid progenitor cells; CHB, chronic hepatitis B; PBMCs, peripheral blood mononuclear cells; BM, bone marrow.

Article Snippet: CD45+EPCs accumulated in AAV/HBV mice model The adeno-associated virus (AAV)/HBV mouse model was established via tail vein injection of 2.5 × 1011 copies of AAV8-HBV1.3 viral genome (Shi et al., 2019) (Packgene, AAV-HBV-002).

Techniques: Injection, Plasmid Preparation, Control, Flow Cytometry, Immunofluorescence, Staining, Giemsa Stain, MANN-WHITNEY

Journal: bioRxiv

Article Title: Brief sleep disruption alters synaptic structures among hippocampal and neocortical somatostatin-expressing interneurons

doi: 10.1101/2024.07.22.604591

Figure Lengend Snippet: Brainbow AAV-transduced SST-CRE mice were sleep-deprived for 6 h via gentle handling ( n = 6), or were allowed to sleep freely in their home cage ( n = 7), starting at lights on. A-B- Control mice slept for more than 60-70% of the first half of the light phase, with an average sleep bout length of approximately 50 min. C – Gentle handling interventions needed to prevent sleep in SD mice (cage taps, cage shakes, and nest disturbances) increased over the course of the 6-h SD experiment. D-F- Representative maximum intensity projection images ( D ) showing Brainbow-labeled SST+ interneurons in each of the examined brain regions, corresponding traced neuronal dendritic arbors ( E ), and detail of inset, highlighting dendritic spines ( F ). Scale bars indicate 100 µm. G- Distribution of spine subtypes for quantified for neurons in each region in freely-sleeping and SD mice.

Article Snippet: At age 3-15 months (Sleep: 7.1 ± 4.9 months; SD: 6.9 ± 4.0 months) each mouse was transduced with a cocktail of CRE recombinase-dependent AAV vectors to express Brainbow 3.0 (AAV-EF1a-BbTagBY and AAV-EF1a-BbChT; titers ≥ 1×1013 vg/mL; Addgene) under isoflurane anesthesia.

Techniques: Gentle, Control, Labeling