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Image Search Results
Journal: Cells
Article Title: Cardiac Remodeling in the Absence of Cardiac Contractile Dysfunction Is Sufficient to Promote Cancer Progression
doi: 10.3390/cells11071108
Figure Lengend Snippet: The tumor promotion phenotype is mediated by secreted factors, expressed in either the heart or the tumor. ( A ) Serum levels of periostin, fibronectin and CTGF were determined via ELISA. ( B , C ) mRNA levels from either the heart ( B ) or tumor ( C ) of the indicated genes were measured by means of qRT-PCR, normalized with GAPDH (heart) or Hsp90 (tumor) house-keeping genes. Data are presented as relative expression compared with control, determined as 1. Data are presented as mean ± SEM and were analyzed via multiple Student’s t -tests. * p < 0.05. ** p < 0.01. *** p < 0.001.
Article Snippet: The quantification of periostin, fibronectin and connective tissue growth factor (CTGF) in the serum was performed using the Mouse Periostin/OSF-2 Quantikine ELISA Kit (R&D systems Inc., Minneapolis, MN, USA),
Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Control
Journal: Applied Microbiology and Biotechnology
Article Title: Direct quantification of lipopeptide biosurfactants in biological samples via HPLC and UPLC-MS requires sample modification with an organic solvent
doi: 10.1007/s00253-017-8272-y
Figure Lengend Snippet: Comparison of PF and SU separation with HPLC and UPLC-MS Systems. PF and SU standards were dissolved in MeOH prior to analysis. Essential peaks are marked with arrows and described. The observed m / z ratios of proton adducts for LP analogs ([M + H] + ]) are shown in the bottom panel
Article Snippet:
Techniques: Comparison
Journal: Applied Microbiology and Biotechnology
Article Title: Direct quantification of lipopeptide biosurfactants in biological samples via HPLC and UPLC-MS requires sample modification with an organic solvent
doi: 10.1007/s00253-017-8272-y
Figure Lengend Snippet: Recovery of LP standards (percent) (mean ± sd, n = 12) dissolved in LB, LB:water, and LB:MeOH during DA. The solvent mixtures were prepared at a 50:50 ( v / v ) ratio. The amount of LP added to the sample was considered to be 100%. The concentration of LP was calculated by comparing the sum of the total peak areas with calibration curves for each LC system. The following HPLC/UPLC-MS configurations or monitoring protocols were used: system 1 (HPLC, absorbance at 210 nm), system 2 (HPLC, absorbance at 210 nm), system 3 DAD (UPLC-MS, absorbance at 210 nm), and system 3 MS TIC (UPLC-MS, TIC detection)
Article Snippet:
Techniques: Solvent, Concentration Assay
Journal: Applied Microbiology and Biotechnology
Article Title: Direct quantification of lipopeptide biosurfactants in biological samples via HPLC and UPLC-MS requires sample modification with an organic solvent
doi: 10.1007/s00253-017-8272-y
Figure Lengend Snippet: Relative recovery of LPs (percent) (mean ± sd, n = 9) from culture broths diluted with water (2× water) or MeOH (2× MeOH and 10× MeOH) in comparison to LPs quantified in undissolved samples (1× sample, 100%). The concentration of LPs was calculated by comparing the sum of the total peak areas with calibration curves for each LC system. The following HPLC/UPLC-MS configurations or monitoring protocols were used: system 1 (HPLC, absorbance at 210 nm), system 2 (HPLC, absorbance at 210 nm), system 3 DAD (UPLC-MS, absorbance at 210 nm), and system 3 MS TIC (UPLC-MS, TIC detection)
Article Snippet:
Techniques: Comparison, Concentration Assay