Journal: Enzyme Research

Article Title: Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate: A Molecular Tool for Remediation

doi: 10.4061/2010/125429

Figure Lengend Snippet: Kinetic parameters of CYP102A2 using SDS and NADPH as substrates. Steady-state kinetic measurements were performed at 37°C in 0.1 M potassium phosphate buffer, pH 7.2. All initial velocities were determined in triplicate. The kinetic parameters K cat and K m for NADPH were calculated by nonlinear regression analysis of experimental steady-state data using the GraFit (Erithacus Software Ltd.) program. The V max value, S 0.5 , and the Hill coefficient, n H , for SDS were determined by fitting the plotted v versus substrate concentration to the Hill equation using the GraFit (Erithacus Software Ltd.) program.

Article Snippet: Kinetic data were analyzed using the computer program GraFit (Erithacus Software Ltd.) [ ].

Techniques: Software, Concentration Assay