Journal: Polymers

Article Title: Innovative Electrospun Nanofiber Mats Based on Polylactic Acid Composited with Silver Nanoparticles for Medical Applications

doi: 10.3390/polym16030409

Figure Lengend Snippet: Percentage of cell viability of L929 mouse fibroblast cells by MTT assay of different mats over 24 h of culture. NC: Cells and culture media without any samples. The experiments were repeated in triplicate.

Article Snippet: L-929 cells, a mouse fibroblast cell line (Elabscience, Houston, TX, USA), were cultured in 96-well plates at a density of 7.5 × 10 4 cells/mL in a completed MEM medium containing 10% FBS and 1% penicillin–streptomycin ( v / v ) and incubated for 24 h in a humidified atmosphere of 5% CO 2 in an incubator at 37 °C.

Techniques: MTT Assay

Journal: International Journal of Molecular Sciences

Article Title: TOMM40 Genetic Variants Cause Neuroinflammation in Alzheimer’s Disease

doi: 10.3390/ijms24044085

Figure Lengend Snippet: TOMM40 genetic variants induce the secretion of pro-inflammatory cytokines in microglial cells, leading to cell death of hippocampal neurons. ( A ) Compared to control cells or cells expressing WT TOMM40, BV2 microglial cells’ expression of (F113L) or (F131L) TOMM40 significantly increased secretion of pro-inflammatory IL-1β, IL-6, or TNF-α in culture medium of BV2 microglial cells. ( B ) Culture medium (CM) of HT22 hippocampal neurons was replaced with CM from BV2 microglial cells transfected with cDNA of WT, (F113L) or (F131L) TOMM40. One day after replacement, CM of BV2 microglia cells expressing mutant (F113L) or (F131L) TOMM40 significantly reduced cell viability of HT22 hippocampal neurons. Each bar represents mean ± S.D. of four experiments. Each experiment was performed in triplicate. * p < 0.05 or ** p < 0.01 compared to control BV2 microglial cells or HT22 hippocampal neurons.

Article Snippet: BV2 mouse microglial cells and HT22 mouse hippocampal neuronal cells were purchased from Elabscience (Cat. EP-ML-0697 and EP-CL-0493) and maintained in a DMEM medium containing 10% FBS.

Techniques: Control, Expressing, Transfection, Mutagenesis