Journal: eLife

Article Title: Live tracking of moving samples in confocal microscopy for vertically grown roots

doi: 10.7554/eLife.26792

Figure Lengend Snippet: The vertically mounted confocal microscope enables long-term imaging of plant roots growing along the gravity vector under controlled illumination. Growing root tips are tracked using the TipTracker MATLAB program. By turning the sample around the optical axis using the rotation stage, gravistimulation is applied to the growing root tips. DOI: http://dx.doi.org/10.7554/eLife.26792.002

Article Snippet: We also provide a compiled version of TipTracker for usage with Zeiss ZEN2010 ( ).

Techniques: Microscopy, Imaging, Plasmid Preparation

Journal: eLife

Article Title: Live tracking of moving samples in confocal microscopy for vertically grown roots

doi: 10.7554/eLife.26792

Figure Lengend Snippet: ( A ) Maximum intensity projections of a single time point for the eight roots tracked. ( B ) Growth rates of the root tips were calculated from the output of the TipTracker program. The yellow and grey areas indicate when the LED illumination was on or off, respectively. ( C ) Cell division and elongation are visualized for the root #5. Each new cell wall is highlighted so that the original cell walls are in yellow, the second generation of the walls is in cyan, the third generation is in magenta, and the fourth generation in green. The last image of the series is shown on the right side. A stack of 14 images (x/y/z: 1400 × 1400 × 14 pixels, voxelsize: 0.457 × 0.457 × 2.5 μm³) was captured every 20 min for a period of 38 h 20 min using the Plan-Apochromat 20x/0.8 air objective lens. Scale bars: ( A ) 100 μm, ( C ) 40 μm. DOI: http://dx.doi.org/10.7554/eLife.26792.008

Article Snippet: We also provide a compiled version of TipTracker for usage with Zeiss ZEN2010 ( ).

Techniques: