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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with <t>anti-NF-jB-p65</t> (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
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Image Search Results


Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with anti-NF-jB-p65 (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).

Journal: Basic & clinical pharmacology & toxicology

Article Title: Effects of insulin-like growth factor-1 on rotenone-induced apoptosis in human lymphocyte cells.

doi: 10.1111/j.1742-7843.2009.00472.x

Figure Lengend Snippet: Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with anti-NF-jB-p65 (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).

Article Snippet: The supplier’s protocol (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA; goat ABC staining System: cat # sc-2023) was followed for the immunocytochemistry using primary goat polyclonal antibodies NF-jB p65 (C-20)-G (Santa Cruz 2009 The Authors Journal compilation 2009 Nordic Pharmacological Society.

Techniques: Activation Assay, Incubation, Staining, Translocation Assay