Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Reactivation of memory-associated neurons induces downstream suppression of competing neuronal populations.

doi: 10.1073/pnas.2410101122

Figure Lengend Snippet: Fig. 1. Schematic illustration of the excitation spectra for GCaMP, ChR, and ChrimsonR with their relative intensities.

Article Snippet: The viruses used in these experiments were pAAV9 cFos- tTA (UMass Vector Core; titer 2.7 × 1013 GC/mL), pAAV9 TRE- mCherry (UMass Vector Core; titer 1 × 1013 GC/mL), AAV9 TRE- ChrimsonR- mCherry (titer 1.1 × 1013 GC/mL), and AAV9 Synapsin GCaMP7f (Addgene; titer 1.3 × 1013 GC/mL).

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Reactivation of memory-associated neurons induces downstream suppression of competing neuronal populations.

doi: 10.1073/pnas.2410101122

Figure Lengend Snippet: Fig. 2. Validation of ChrimsonR for optogenetic reactivation of memory-associated neurons. Head-fixed optogenetically induced memory reactivation. (A) Schematic showing the experimental process. (B) Licking values for each mouse across the trial, with the means and SD shown. (C) Quantification of the change in freezing from the final stimulus on to the final stimulus off epoch (n = 5 FC− animals, n = 6 FC+ animals; P = 0.02 Wilcoxon Rank Sum Test). (D) Representative histological images, arrows point to overlapping cells. (Scale bar, 100 µm.)

Article Snippet: The viruses used in these experiments were pAAV9 cFos- tTA (UMass Vector Core; titer 2.7 × 1013 GC/mL), pAAV9 TRE- mCherry (UMass Vector Core; titer 1 × 1013 GC/mL), AAV9 TRE- ChrimsonR- mCherry (titer 1.1 × 1013 GC/mL), and AAV9 Synapsin GCaMP7f (Addgene; titer 1.3 × 1013 GC/mL).

Techniques: Biomarker Discovery

Journal: STAR Protocols

Article Title: Protocol to drive human monocyte-to-macrophage polarization in vitro using tumor conditioned media

doi: 10.1016/j.xpro.2022.101666

Figure Lengend Snippet:

Article Snippet: MACS Comp Bead Kit, anti-mouse Igκ , Miltenyi Biotec , 130-097-900.

Techniques: Recombinant, Saline, Software, Bicinchoninic Acid Protein Assay, Filtration, Microscopy, Cell Culture, Flow Cytometry, Spectrophotometry