Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: HPLC conditions for quantifying minor alkaloids.

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: Contents of minor alkaloids in fall and summer P. amurense stems quantified by HPLC.

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: Cryo-TOF-SIMS spectra and chemical structures of (A) berberine, (B) columbamine, (C) jatrorrhizine, (D) magnoflorine, (E) phellodendrine, (F) 8-oxoepiberberine, (G) palmatine and (H) tetrahydropalmatine. Cryo-TOF-SIMS spectra obtained from the frozen, hydrated transverse surface of a P. amurense stem from (I) fall and (J) summer in the region containing phloem, cambial zone, and xylem. Alkaloid standard chemicals were dissolved at ca. 50 mM in ACN and frozen for measurements.

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: Cryo-TOF-SIMS/SEM images of a freeze-fixed stem of P. amurense in fall at the transverse surface. (A) Cryo-SEM image of the freeze-etched sample after cryo-TOF-SIMS analysis. Cryo-TOF-SIMS images for positive ions of (B) total ions, (C) potassium at m / z 39, (D) phosphatidylcholine at m / z 58, (E) berberine at m / z 336, (F) columbamine and jatrorrhizine at m / z 338, (G) magnoflorine and phellodendrine at m / z 342, (H) 8-oxoepiberberine and palmatine at m / z 352, (I) tetrahydropalmatine at m / z 356. (J) An optical microscopy image of a freeze-fixed stem of P. amurense from the fall season in the sample holder showing the measured area. Scale bars are 200 μm for (A-I) and 1.0 mm for (J) .

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques: Microscopy

Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: Cryo-TOF-SIMS/SEM images of a freeze-fixed stem of P. amurense in summer at the transverse surface. (A) Cryo-SEM image of the freeze-etched sample after cryo-TOF-SIMS analysis. Cryo-TOF-SIMS images for positive ions of (B) total ions, (C) potassium at m / z 39, (D) phosphatidylcholine at m / z 58, (E) berberine at m / z 336, (F) columbamine and jatrorrhizine at m / z 338, (G) magnoflorine and phellodendrine at m / z 342, (H) 8-oxoepiberberine and palmatine at m / z 352, (I) tetrahydropalmatine at m / z 356. (J) Optical microscopy image of a freeze-fixed stem of P. amurense from the summer season in the sample holder showing the measured area. Scale bars are 200 μm for (A−I) and 1.0 mm for (J) .

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques: Microscopy

Journal: Pharmaceuticals

Article Title: Sinomenium acutum Modulates Platelet Aggregation and Thrombus Formation by Regulating the Glycoprotein VI-Mediated Signalosome in Mice

doi: 10.3390/ph17010006

Figure Lengend Snippet: Phytochemical components of Sinomenium acutum via UHPLC-MS/MS.

Article Snippet: The latter included higenamine, gelsemine, magnoflorine, scopoletin, columbamine, jatrorrhizine, palmatine, syringin, and eleutheroside E, which were purchased from TargetMol (Wellesley Hills, MA, USA).

Techniques:

Journal: Pharmaceuticals

Article Title: Sinomenium acutum Modulates Platelet Aggregation and Thrombus Formation by Regulating the Glycoprotein VI-Mediated Signalosome in Mice

doi: 10.3390/ph17010006

Figure Lengend Snippet: Ultra-high-performance liquid chromatography–tandem mass spectrometry examination of Sinomenium acutum . ( A ) Base peak chromatogram of Sinomenium acutum in positive and negative ion mode. ( B ) Elicit ion chromatogram of Sinomenium acutum . 1, higenamine; 2, acutumidine; 3, acutumine; 4, sinomenine; 5, gelsemine; 6, magnoflorine; 7, scopoletin; 8, columbamine or jatrorrhizine; 9, palmatine; 10, syringin; 11, eleutheroside E.

Article Snippet: The latter included higenamine, gelsemine, magnoflorine, scopoletin, columbamine, jatrorrhizine, palmatine, syringin, and eleutheroside E, which were purchased from TargetMol (Wellesley Hills, MA, USA).

Techniques: High Performance Liquid Chromatography, Mass Spectrometry

Journal: Frontiers in Plant Science

Article Title: Study on the correlation between alkaloids and tastes of Coptis Rhizome from four species based on UHPLC-QQQ-MS/MS combined with electronic tongue technique

doi: 10.3389/fpls.2024.1496789

Figure Lengend Snippet: The representative MRM chromatograms of mixed reference solution and samples of Coptis rhizome. (A) mixed reference solution, (B) test solution of Coptis chinensis rhizome, (C) test solution of Coptis deltoidea rhizome, (D) test solution of Coptis teeta rhizome, and (E) test solution of Coptis japonica rhizome. 1. magnoflorine, 2. tetrahydricheilanthifolinium, 3. coptisine, 4. epiberberine, 5. columbamine, 6. jatrorrhizine, 7. berberrubine, 8. berberine, 9. palmatine, 10. berlambine.

Article Snippet: Chemical reference substances (CRS), such as magnoflorine, tetrahydricheilanthifolinium, coptisine, epiberberine, columbamine, jatrorrhizine, berberrubine, berberine, palmatine, berlambine (purity ≥98%, HPLC grade), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques:

Journal: Frontiers in Plant Science

Article Title: Study on the correlation between alkaloids and tastes of Coptis Rhizome from four species based on UHPLC-QQQ-MS/MS combined with electronic tongue technique

doi: 10.3389/fpls.2024.1496789

Figure Lengend Snippet: The contents of ten alkaloids from four species of Coptis rhizome.

Article Snippet: Chemical reference substances (CRS), such as magnoflorine, tetrahydricheilanthifolinium, coptisine, epiberberine, columbamine, jatrorrhizine, berberrubine, berberine, palmatine, berlambine (purity ≥98%, HPLC grade), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques:

Journal: Frontiers in Plant Science

Article Title: Study on the correlation between alkaloids and tastes of Coptis Rhizome from four species based on UHPLC-QQQ-MS/MS combined with electronic tongue technique

doi: 10.3389/fpls.2024.1496789

Figure Lengend Snippet: The correlation analysis of tastes and alkaloids for Coptis rhizome.

Article Snippet: Chemical reference substances (CRS), such as magnoflorine, tetrahydricheilanthifolinium, coptisine, epiberberine, columbamine, jatrorrhizine, berberrubine, berberine, palmatine, berlambine (purity ≥98%, HPLC grade), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Berberine-microbiota interplay: orchestrating gut health through modulation of the gut microbiota and metabolic transformation into bioactive metabolites

doi: 10.3389/fphar.2023.1281090

Figure Lengend Snippet: Berberine and berberine-derived metabolites in plasma (top), ileum (middle) or caecum (bottom) from chickens fed a berberine-supplemented diet (0.1, 0.5 or 1 g/kg feed) for 21 days post-hatch (n = 12). (A) Log 10 concentration profiles of berberine, phase I and cumulated phase II berberine metabolites. (B) Concentration profiles of phase II glucuronide and sulfate berberine metabolites. The shade area represents the 95% confidence interval. Total glucuronides and sulfates represent cumulated phase II berberine metabolites and include berberrubine-glucuronide/-sulfate, columbamine-glucuronide/-sulfate, jatrorrhizine-glucuronide/-sulfate, demethyleneberberine-glucuronide/-sulfate01/02/03/04/05/06, thalifendine-glucuronide/-sulfate. Oxyberberine, columbamine, demethyleneberberine_sulfate01/02/05/06 were not detected in the plasma. Colu-gluc/-sulf: columbamine-glucuronide/-sulfate; Jatr-gluc/-sulf: jatrorrhizine-glucuronide/-sulfate; Deme-gluc/-sulf: demethyleneberberine-glucuronide/-sulfate; Thal-gluc: thalifendine-glucuronide; Berb-gluc/-sulf: berberrubine-glucuronide/-sulfate.

Article Snippet: Demethyleneberberine (purity >98%), columbamine (purity >98%) and dihydroberberine (purity >95%), were purchased at AbMole BioScience (Brussels, Belgium).

Techniques: Derivative Assay, Clinical Proteomics, Concentration Assay

Journal: Frontiers in Pharmacology

Article Title: Berberine-microbiota interplay: orchestrating gut health through modulation of the gut microbiota and metabolic transformation into bioactive metabolites

doi: 10.3389/fphar.2023.1281090

Figure Lengend Snippet: Comparison of log 10 concentration profiles of berberine and berberine-derived metabolites between ileum (yellow) and caecum (purple) from chickens fed a berberine-supplemented diet (0.1, 0.5 or 1 g/kg feed) for 21 days post-hatch (n = 12). Total glucuronides and sulfates represent cumulated phase II berberine metabolites and include berberrubine-glucuronide/-sulfate, columbamine-glucuronide/-sulfate, jatrorrhizine-glucuronide/-sulfate, demethyleneberberine-glucuronide/-sulfate_01/_02/_03/_04/_05/_05/_06, thalifendine-glucuronide/-sulfate. reports concentrations profiles in plasma.

Article Snippet: Demethyleneberberine (purity >98%), columbamine (purity >98%) and dihydroberberine (purity >95%), were purchased at AbMole BioScience (Brussels, Belgium).

Techniques: Comparison, Concentration Assay, Derivative Assay, Clinical Proteomics