Article Title: Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination
Figure Lengend Snippet: Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination. (A) Detection of SARS-CoV-2 with different concentrations of azure II-pheno red combined dye. (B) Sensitivity and accuracy of SARS-CoV-2 detection, WarmStart® Colorimetric LAMP 2X Master Mix (New England Biolabs) as a reference with diluted patient samples (duplicates of 10 9 , 10 8 , 10 7 , 10 6 , 10 5 , 10 4 and 10 3 copies of RNA as well as non template control (NTC)). (C) Summary of SARS-CoV-2 detection for diluted patient samples, WarmStart® Colorimetric LAMP 2X Master Mix (New England Biolabs) as a reference, color indicates time of detection of amplifying, gray indicates no amplification of no template control.
Article Snippet: In addition to the commercial reverse transcriptase (RT) and Bst enzyme or WarmStart® Colorimetric LAMP 2X Master Mix, we used the home-made RT and Bst (Bst V7.16) to perform iLACO [ , ]. iLACO reactions were performed using the home-made solution without Tris-HCl, containing only a small amount of Tris-HCl carried over from the DNA polymerase storage buffer.