Journal: European Journal of Medical Research

Article Title: NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation

doi: 10.1186/s40001-025-03397-3

Figure Lengend Snippet: NDC80 modulated tumor-associated macrophages polarization to promote ESCC progression. ( A ) Immunohistochemistry was used to detect the expression of NDC80 protein and macrophage markers in ESCC tumor tissue, n = 61. B, C Correlation between the expression of NDC80 protein and the number of macrophages infiltrations in ESCC tumor tissue was analyzed by simple linear regression, n = 61. Flow cytometry ( D ) and qPCR ( E ) were used to detect the regulation of macrophages polarization by ESCC cells overexpressing NDC80, n = 3, multiple unpaired t tests. F qPCR was used to detect the regulation of macrophages polarization by ESCC cells with silenced NDC80, n = 3, multiple unpaired t tests. G, H qPCR was used to assay the mRNA expression levels of inflammatory cytokines in tumor cells with different NDC80 expression levels, n = 3, multiple unpaired t tests. I, J ELISA detection of M-CSF and CXCL-2 levels in the supernatant of ESCC cells with overexpression or knockdown of NDC80 protein, n = 3, multiple unpaired t tests. M-CSF: Standard curve range = 31.25—2000 pg/mL, R 2 = 0.9997. CXCL-2: Standard curve range = 15.63—1000 pg/mL, R 2 = 0.9986. (*, p < 0.05; **, p < 0.01; ***, p < 0.001)

Article Snippet: The concentrations of cytokines M-CSF (E-EL-H0097) and CXCL-2 (E-EL-H1904) in the cell supernatants were detected using ELISA kits (Elabscience, China).

Techniques: Immunohistochemistry, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Over Expression, Knockdown

Journal: Acta biomaterialia

Article Title: Spatial targeting of fibrosis-promoting macrophages with nanoscale metal-organic frameworks for idiopathic pulmonary fibrosis therapy.

doi: 10.1016/j.actbio.2023.12.006

Figure Lengend Snippet: Fig. 1. Overexpression of CSF-1R in CD206+ M2 macrophages. (A) Flow cytometry analysis of CSF-1R and CD206 expression in F4/80+ macrophages. Right panels: Quantified data in sorted F4/80+ macrophages. Results are expressed as means ± SD ( n = 3; ∗∗P < 0.01). (B) Representative results for coimmunostaining of CD206 and CSF-1R in the lung sections from bleomycin-treated mice. Representative images are shown. Bar = 20 μm. (C) Immunohistochemistry staining of CSF-1R and CD206 in the lung sections of IPF patients. Representative images are shown. Bar = 50 μm. (D-F) Single cell atlas of IPF patients according to dataset GSE122960. (D) Upper panels: Cellular populations identified. Lower panels: t-distributed stochastic neighbor embedding (t-SNE) depicting cell clusters originating either from a donor or from IPF patients. (E) Expression of CSF-1R for the cell types defined above each panel. (F) Percentage of cells with non-zero CSF-1R expression. (G) Kaplan– Meier survival analyses of IPF patients based on the expression of CSF-1R according to dataset GSE70866. (H) Immunofluorescence staining of CSF-1R and α-SMA on mouse lung tissues. Representative images are shown. Bar = 50 μm.

Article Snippet: The primary antibodies used were rabbit nti-phospho-CSF-1R, mouse anti- β-actin, and rabbit anti-GAPDH. horseradish peroxidase-conjugated goat anti-mouse/rabbit IgG Boster no. BA1050/BA1056, Wuhan, China, 1:10,0 0 0 dilution) was sed as the secondary antibody.

Techniques: Over Expression, Flow Cytometry, Expressing, Immunohistochemistry, Staining

Journal: Acta biomaterialia

Article Title: Spatial targeting of fibrosis-promoting macrophages with nanoscale metal-organic frameworks for idiopathic pulmonary fibrosis therapy.

doi: 10.1016/j.actbio.2023.12.006

Figure Lengend Snippet: Fig. 5. (A) Schematic diagram of the BLZ-loaded NPs therapy procedure. (B) Inhibitory effects of scrNP-BLZ@Mn-Cur or M2NP-BLZ@Mn-Cur on CSF-1R phosphorylation in lung tissues. (C) Representative flow cytometry results is shown to identify pulmonary macrophage subsets from lung digests in BLM-treated mice. Right panels: Quantified data in sorted F4/80+ macrophages ( n = 3, means ± SD). (D) Cell differential from BAL fluid was counted by Diff-Quik staining ( n = 5; mean ± SD; ∗p < 0.05). (E) The level of TGF- β1, IL-6, IL-11, and TNF- α in lung tissues were determined by ELISA. Results are expressed as means ± SD ( n = 3; ∗∗p < 0.01, ∗p < 0.05).

Article Snippet: The primary antibodies used were rabbit nti-phospho-CSF-1R, mouse anti- β-actin, and rabbit anti-GAPDH. horseradish peroxidase-conjugated goat anti-mouse/rabbit IgG Boster no. BA1050/BA1056, Wuhan, China, 1:10,0 0 0 dilution) was sed as the secondary antibody.

Techniques: Phospho-proteomics, Cytometry, Diff-Quik, Staining, Enzyme-linked Immunosorbent Assay