Journal: Saudi Journal of Biological Sciences

Article Title: Establishment of apoptotic regulatory network for genetic markers of colorectal cancer

doi: 10.1016/j.sjbs.2017.01.014

Figure Lengend Snippet: Effect of transfecting WWOX into Colo205 cell line on cell apoptosis.

Article Snippet: Human colorectal cancer cell line Colo205 was purchased in China Center for Type Culture Collection, and verification experiment was conducted after cells’ arrival.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Establishment of apoptotic regulatory network for genetic markers of colorectal cancer

doi: 10.1016/j.sjbs.2017.01.014

Figure Lengend Snippet: RT-PCR analysis on effect of WWOX on p53 mRNA expression; Note: 1: Colo205 cell line control group; 2: pcDNA4.0/Myc-His empty vector-transfected group; 3: pcDNA4.0/Myc-WWOX transfection group.

Article Snippet: Human colorectal cancer cell line Colo205 was purchased in China Center for Type Culture Collection, and verification experiment was conducted after cells’ arrival.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Plasmid Preparation, Transfection

Journal: Mucosal Immunology

Article Title: TNF hampers intestinal tissue repair in colitis by restricting IL-22 bioavailability

doi: 10.1038/s41385-022-00506-x

Figure Lengend Snippet: A TNF blockade increases levels of bioactive colonic IL-22 that can be inhibited by recombinant IL-22BP. Supernatants derived from colonic explants of Rag1 −/− mice which received naive hTNF-KI T cells and treated for 2 weeks with anti-TNF, were added to human colonic Colo205 cell line with or without rmIL-22BP (1,25 mg/ml). Production of human IL-10 as an indicator of bioactive IL-22 has been determined 48 h later. B Expression of IL-22BP mRNA in sorted CD45 + CD11c + MHCII + CD103 + and CD45 + TCRβ + cells from the colon of mice treated with Fc control or infliximab for 2 weeks. Naive hTNF-KI T cells were transferred to Rag1 −/− recipients, anti-TNF (infliximab; 10 mg/kg; i.p. twice per week) was administered once mice had lost 5% of their initial weight. C Expression of IL-22BP in colon and spleen from naive WT and TNF −/− mice. D Expression of IL-22BP in spleen from naive WT, T-TNF −/− , B-TNF −/− , and M-TNF −/− mice. E Expression of IL-22BP in sorted CD4 + CD11c + MHCII + or CD8 + CD11c + MHCII + cells isolated from spleen of naïve WT and TNF −/− mice. CD4 + CD11c + MHCII + cells from TNF −/− mice were incubated with rmTNF (100 ng/ml; 4 h). F Levels of human IL-22BP in human monocyte-derived DCs (moDCs) stimulated with TNF (100 ng/ml), LPS (100 ng/ml), PAM 3 Cys (5 mcg/ml) for indicated times. G Correlation between hTNF and hIL-22BP levels in sera of IBD patients. All mouse data are representative of two independent experiments. Data represent mean values ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, as calculated by Student’s t-test; ns not significant. The Pearson correlation was used for correlative analyses. The significance level was set to p ≤ 0.05.

Article Snippet: Colo205 cell line (provided by Dr. Verena Moos, Charite, Berlin; RRID: CVCL_0218) was used to estimate IL-22 bioactivity.

Techniques: Recombinant, Derivative Assay, Expressing, Control, Isolation, Incubation