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Image Search Results
Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Article Title: Role of TGF-β1/SMADs signalling pathway in resveratrol-induced reduction of extracellular matrix deposition by dexamethasone-treated human trabecular meshwork cells
doi: 10.4196/kjpp.2024.28.4.345
Figure Lengend Snippet: Effect of TR on the (A) COLIA1 gene (B) COLIA2 gene (C) COLI protein (D) COLIIIA1 gene (E) COLIII protein (F) COLIVA1 gene (G) COLIVA2 gene (H) COLIV protein (I) FN1 gene (J) Fibronectin protein (K) ACTA2 gene and (L) Alpha smooth muscle actin protein expressions by the DEXA-treated HTMCs after treatment for three and seven days. Bars represent mean ± SD (n = 3). *p < 0.05, ANOVA with Tukey’s post-hoc. TR, resveratrol; ECM, extracellular matrix; DEXA, dexamethasone; HTMC, human trabecular meshwork cell; DMEM, Dulbecco’s modified Eagle medium; DMSO, dimethyl sulfoxide.
Article Snippet: These proteins included COLI, total and active TGF-β1, SMAD4, SMAD7, PAI-1 and tPA (Finetest),
Techniques: Modification
Journal: Parasites & Vectors
Article Title: MicroRNA-497 induced by Clonorchis sinensis enhances the TGF-β/Smad signaling pathway to promote hepatic fibrosis by targeting Smad7
doi: 10.1186/s13071-021-04972-3
Figure Lengend Snippet: Inhibition of miR-497 in mice reduced CCl 4 -induced liver fibrosis. a Collagen deposition shown by Masson staining in the livers from different groups as indicated. b Sirius red staining showed collagen deposition in the livers from different groups as indicated. c Hydroxyproline (HYP) content in the liver of mice in each group as indicated. d Levels of α-SMA, P-Smad2/3, COLI, and COLIII in in the livers from different groups determined by western blot. * P < 0.05, ** P < 0.01, compared with the indicated group. All data represent at least three independent experiments
Article Snippet: The membranes were cut into bands of appropriate width according to the protein marker and after 60 min of blocking with 5% fat-free milk, membranes were incubated with Smad 7 antibody (1:2000; Sigma, USA), COLI antibody (1:2000; Sigma, USA), P-Smad2/3 antibody (1:1000; Abclonal, Wuhan, China),
Techniques: Inhibition, Staining, Western Blot