Journal: Emerging Infectious Diseases

Article Title: Zoonotic and Anthroponotic Plasmodium spp. Circulation between Wild Primates and Indigenous Community, Peruvian Amazon, 2007–2020

doi: 10.3201/eid3205.251695

Figure Lengend Snippet: Workflow for molecular detection and validation of Plasmodium spp. infections in study of zoonotic and anthroponotic Plasmodium spp. circulation between wild primates and Indigenous community, Peruvian Amazon, 2007–2020. Schematic of laboratory procedures show DNA extraction and detection of Plasmodium spp. in human and NHP blood samples using qPCR (targeting 18S rRNA gene) and nPCR targeting mitochondrial genes ( cytb and cox3 ). Selected samples underwent sequencing for species confirmation and external validation. cytb , cytochrome oxidase b; cox3 , cytochrome c oxidase III; NHP, nonhuman primate; nPCR, nested PCR; qPCR, quantitative PCR.

Article Snippet: We detected parasites by using 2 nested PCRs and 2 commercial VIASURE qPCR diagnostic kits (Certest Biotec, https://www.certest.es ) targeting mitochondrial cytochrome oxidase b ( cytb ), cytochrome c oxidase III ( cox3 ), and 18S SSU rRNA genes.

Techniques: Biomarker Discovery, DNA Extraction, Sequencing, Nested PCR, Real-time Polymerase Chain Reaction

Journal: Emerging Infectious Diseases

Article Title: Zoonotic and Anthroponotic Plasmodium spp. Circulation between Wild Primates and Indigenous Community, Peruvian Amazon, 2007–2020

doi: 10.3201/eid3205.251695

Figure Lengend Snippet: Age-specific parasite load in humans based on quantitative PCR targeting for Plasmodium spp. 18S rRNA genes in study of zoonotic and anthroponotic Plasmodium spp. circulation between wild primates and Indigenous community, Peruvian Amazon, 2007–2020. A) Smoothed regression of log-transformed parasite DNA concentration, by age, in 49 persons. B) Boxplot comparing parasite loads between children < 8 years and older persons. Higher parasite loads occurred in younger age groups. Horizontal lines within boxes indicate medians; box tops and bottoms indicate interquartile range (25th–75th percentiles); error bars indicate 1.5 × interquartile range.

Article Snippet: We detected parasites by using 2 nested PCRs and 2 commercial VIASURE qPCR diagnostic kits (Certest Biotec, https://www.certest.es ) targeting mitochondrial cytochrome oxidase b ( cytb ), cytochrome c oxidase III ( cox3 ), and 18S SSU rRNA genes.

Techniques: Real-time Polymerase Chain Reaction, Transformation Assay, Concentration Assay

Journal: Scientific Reports

Article Title: BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene

doi: 10.1038/s41598-017-12342-2

Figure Lengend Snippet: Effects of BRD4 depletion on expression of genes related to insulin sensitivity in 3T3-L1 adipocytes. Control or Brd4 shRNA-expressing 3T3-L1 cells were treated with medium for differentiation and real-time RT-PCR analyses for Albp , Glut4 , Fas , Accα , Accβ , Dgat1 , Lpl , Hsl , Aco , Pparγ1 , Pparγ2 , Creb , C/ebpα , C/ebpβ , C/ebpδ , C/ebpγ , C/ebpζ , Chrebp , Pgc1α , Srebp1 , Srebp1α , Lxrα and Lxrβ were performed at 2 and 8 days after differentiation stimulation. The data shown are means ± SEM of 6 wells per condition in a single experiment. * P < 0.05, ** P < 0.01 versus the corresponding control cells by Student’s t -test.

Article Snippet: BAC clones containing the murine Brd4 locus were isolated using the Do-It-Yourself PCR screening kit (Mouse ES BAC, Release I) (Incyte, Wilmington, DE).

Techniques: Expressing, Control, shRNA, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene

doi: 10.1038/s41598-017-12342-2

Figure Lengend Snippet: Effects of BRD4 overexpression on Brd4 mRNA and protein levels and mRNA levels of genes related to insulin sensitivity in 3T3-L1 adipocytes. Brd4 -overexpressing or control plasmid transfected 3T3-L1 cells were treated with medium for differentiation. Immunoblot analysis for BRD4, CYCLIN T1, CDK9, PPARγ2 and TBP and real-time RT-PCR analyses for Brd4 , Adipoq , Albp , Glut4 , Fas , Accα , Accβ , Dgat1 , Lpl , Hsl , Aco , Pparγ1 , Pparγ2 , Creb , C/ebpα , C/ebpβ , C/ebpδ , C/ebpγ , C/ebpζ , Chrebp , Pgc1α , Srebp1 , Srebp1α , Lxrα and Lxrβ were performed in samples at 2 days after differentiation. The data shown are means ± SEM of 6 wells per condition in a single experiment. * P < 0.05, ** P < 0.01 versus the corresponding control cells by Student’s t -test.

Article Snippet: BAC clones containing the murine Brd4 locus were isolated using the Do-It-Yourself PCR screening kit (Mouse ES BAC, Release I) (Incyte, Wilmington, DE).

Techniques: Over Expression, Control, Plasmid Preparation, Transfection, Western Blot, Quantitative RT-PCR