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Image Search Results
Journal: Inflammatory bowel diseases
Article Title: miR-4728-3p functions as a tumor suppressor in ulcerative colitis-associated colorectal neoplasia through regulation of focal adhesion signaling
doi: 10.1097/MIB.0000000000001104
Figure Lengend Snippet: Expression correlation between miR-4728-3p and CAV1 (A), COL1A2 (B), and THBS2 (C) by microarray analysis. Quantitative real-time PCR was performed to validate expression of miR-4728-3p (D) as well as CAV1, COL1A2, and THBS2 (E) in the same 42 patients analyzed by microarray. Quantitative real-time PCR was also performed in 11 UC-associated cancers, nondysplastic adjacent tissue, and normal-appearing control tissues for miR-4728-3p (F) as well as CAV1, COL1A2, and THBS2 (G). Error bars represent standard error of the mean.
Article Snippet: Primary antibodies were diluted to 1:100 for CAV1 (Santa Cruz Biotechnology), 1:500 for
Techniques: Expressing, Microarray, Real-time Polymerase Chain Reaction, Control
Journal: Inflammatory bowel diseases
Article Title: miR-4728-3p functions as a tumor suppressor in ulcerative colitis-associated colorectal neoplasia through regulation of focal adhesion signaling
doi: 10.1097/MIB.0000000000001104
Figure Lengend Snippet: A) Representative Western blots and B) quantitative expression by densitometry of CAV1, COL1A2, and THBS2 in the indicated colon cancer cell line following transfection with miR-4728-3p or scrambled oligo (n=3/treatment group). C) Firefly luciferase activity after transfection with miR-4728-3p and following treatment of indicated genes regulated by wild type or mutant 3′UTR (n=6/treatment group). To control for transfection efficiency, firefly luciferase activity was normalized to renilla luciferase activity. D) Representative images of wound healing in HCT116 cells treated with miR-4728-3p or a scrambled probe. E) Quantitative measurement of cell migration to assess wound closure in HCT116 cells treated with miR-4728-3p or a scrambled probe (values are means ± SD of 3 independent platings). F) Representative immunofluorescence images of vinculin and actin to assess focal adhesion complexes and actin cytoskeleton in HCT116 cells following treatment with miR-4728-3p or a scrambled probe. G) Focal adhesion intensity and H) area following treatment with miR-4728-3p or a scrambled probe (n=3 wells with 12 measurements each/treatment group). Error bars represent standard deviation.
Article Snippet: Primary antibodies were diluted to 1:100 for CAV1 (Santa Cruz Biotechnology), 1:500 for
Techniques: Western Blot, Expressing, Transfection, Luciferase, Activity Assay, Mutagenesis, Control, Migration, Immunofluorescence, Standard Deviation