Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Higenamine, a Potent Compound from Aconitum , on UVB-Induced Photoaging in Hairless Mice

doi: 10.1155/2022/9116642

Figure Lengend Snippet: Promotive effect of higenamine on the collagen-related proteins. The levels of TGF- β (a), Smad3 (b), and COL1A1 (c) determined by ELISA. Values are expressed as the mean ± standard error of the mean. ∗ P < 0.05 and ∗∗ P < 0.01 compared to the control group.

Article Snippet: The COL1A1 ELISA kit was obtained from Cusabio Technology (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Higenamine, a Potent Compound from Aconitum , on UVB-Induced Photoaging in Hairless Mice

doi: 10.1155/2022/9116642

Figure Lengend Snippet: Effect of higenamine on UVB-induced photoaging in the skin of mice. Representative histological analysis of skin section damaged by UVB exposure. (a) Masson's trichrome staining to identify collagen fibers. The levels of Smad3 (b) and COL1A1 (c) determined by ELISA. Values are expressed as the mean ± standard error of the mean. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared to the control group, and ## P < 0.01 and ### P < 0.001 compared to the UVB-alone group.

Article Snippet: The COL1A1 ELISA kit was obtained from Cusabio Technology (Wuhan, China).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Higenamine, a Potent Compound from Aconitum , on UVB-Induced Photoaging in Hairless Mice

doi: 10.1155/2022/9116642

Figure Lengend Snippet: Effect of higenamine on the levels of UVB-induced photoaging in vitro. Cytotoxicity levels measured by MTT (a), and COL1A1 levels measured by ELISA (b) in cells treated with higenamine followed by UVB stimulation. Levels of Smad2 DNA-binding Phosphorylation (c) and COL1A1 (d) in TGF- β siRNA-transfected differentiated human primary fibroblast cells. Values are expressed as the mean ± standard error of the mean. # P < 0.05 and ### P < 0.001 compared to the control group, ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared to the UVB-alone group, & P < 0.05 compared to the si-TGF- β group, and @ P < 0.05 compared to the higenamine + si-TGF- β + UVB group.

Article Snippet: The COL1A1 ELISA kit was obtained from Cusabio Technology (Wuhan, China).

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Binding Assay, Phospho-proteomics, Transfection, Control

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram Exerts Antifibrotic and Anti-Inflammatory Therapeutic Effects on Perimysial Orbital Fibroblasts in Graves’ Orbitopathy

doi: 10.3390/ijms23095261

Figure Lengend Snippet: DSF exerts antifibrotic effects on pOFs in the GO group. ( A ) The mRNA levels of fibrotic and extracellular matrix production markers (ACTA2, FN1, CTGF, TIMP-1, COL1A1, COL1A2, COL2A1, and COL3A1) were measured, n = 5. ( B ) The protein expression levels of the indicated fibrotic markers in each group were assessed. ( C ) The protein levels were quantified, analyzed, and normalized to the level of GAPDH for each sample, n = 3. ( D ) IF staining of pOFs with FN1 (red), COL1A1 (green), and α-SMA (pink) antibodies after treatment with TGF-β1 (10 ng/mL)/DSF (4 μM). Cell nuclei were stained with DAPI (blue). The stained cells were examined under a fluorescence microscope (200×); scale bar = 50 μm. ( E ) Photographed collagen gel contraction of pOFs after treatment with TGF-β1 (10 ng/mL)/DSF (2 μM, 4 μM) for 0 h, 24 h, and 48 h. ( F ) Statistical analysis of the percentage with respect to the initial area, n = 3. ( G ) Photographed wound repairability of pOFs after treatment with TGF-β1 (10 ng/mL)/DSF (2 μM, 4 μM). Scale bar = 100 μm. ( H ) Statistical analysis of the rate of wound closure, n = 3. The data are expressed as the triplicates’ mean ± standard deviation (SD). # p < 0.05, ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared with the control; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with TGF-β1 alone; ns denotes no statistical significance versus the control/TGF-β1; assessed by one-way ANOVA.

Article Snippet: The following primary antibodies were used: GAPDH (#5174S), α-SMA (#19245S), FN1 (#26836S), COL1A1 (#39952S), CTGF (#86641S), Smad2 (#5339S), phospho-Smad2 (p-Smad2, #3108S), Smad3 (#9523S), phospho-Smad3 (p-Smad3, #9520S), ERK (#4695S), phospho-ERK (p-ERK, #4370S), and Snail (#3879S) (all from Cell Signaling Technology, Boston, MA, USA).

Techniques: Expressing, Staining, Fluorescence, Microscopy, Standard Deviation, Control

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram Exerts Antifibrotic and Anti-Inflammatory Therapeutic Effects on Perimysial Orbital Fibroblasts in Graves’ Orbitopathy

doi: 10.3390/ijms23095261

Figure Lengend Snippet: qPCR primer sequences.

Article Snippet: The following primary antibodies were used: GAPDH (#5174S), α-SMA (#19245S), FN1 (#26836S), COL1A1 (#39952S), CTGF (#86641S), Smad2 (#5339S), phospho-Smad2 (p-Smad2, #3108S), Smad3 (#9523S), phospho-Smad3 (p-Smad3, #9520S), ERK (#4695S), phospho-ERK (p-ERK, #4370S), and Snail (#3879S) (all from Cell Signaling Technology, Boston, MA, USA).

Techniques: