col i Search Results


col i  (Rockland Immunochemicals)
94
Rockland Immunochemicals col i
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Col I, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col-i  (Haemochrom Diagnostica)
90
Haemochrom Diagnostica col-i
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Col I, supplied by Haemochrom Diagnostica, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-human collagen type i col i  (Merck KGaA)
90
Merck KGaA anti-human collagen type i col i
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Anti Human Collagen Type I Col I, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eln, col-i, mmp-1, mmp3, and mmp-9 assay kits  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
90
Nanjing Jiancheng Bioengineering Research Institute Co Ltd eln, col-i, mmp-1, mmp3, and mmp-9 assay kits
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Eln, Col I, Mmp 1, Mmp3, And Mmp 9 Assay Kits, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eln, col-i, mmp-1, mmp3, and mmp-9 assay kits/product/Nanjing Jiancheng Bioengineering Research Institute Co Ltd
Average 90 stars, based on 1 article reviews
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microtiter plates mtps coated with col i  (Corning Life Sciences)
90
Corning Life Sciences microtiter plates mtps coated with col i
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Microtiter Plates Mtps Coated With Col I, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col-i antibodies  (Becton Dickinson)
90
Becton Dickinson col-i antibodies
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Col I Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col i  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company col i
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Col I, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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dpt-exos+scaps+col-i  (SCAPS GmbH)
90
SCAPS GmbH dpt-exos+scaps+col-i
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Dpt Exos+Scaps+Col I, supplied by SCAPS GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against col-i  (Arcis Biotechnology)
90
Arcis Biotechnology antibodies against col-i
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Antibodies Against Col I, supplied by Arcis Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col i/ iii-coated membranes  (Corning Life Sciences)
90
Corning Life Sciences col i/ iii-coated membranes
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Col I/ Iii Coated Membranes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col-i (3)  (KOKEN CO)
90
KOKEN CO col-i (3)
a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar <t>COLs,</t> <t>immunostaining</t> of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Col I (3), supplied by KOKEN CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ocn elisa kit  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
90
Nanjing Jiancheng Bioengineering Research Institute Co Ltd ocn elisa kit
In vitro biological activity test of IWBLHs. (A) The cell toxicity result of extract of various IWBLHs. (B) The BMSCs adhesion results of IWBLHs. (C) BMSCs proliferation result of IWBLHs. (D) ALP and (E) <t>OCN</t> of BMSCs growing on various IWBLHs, the weight ratio indicated SA: ACP: mineralized collagen fibril ( n = 3, experiments performed in triplicate. The bars show the mean ± SD, * p < 0.05).
Ocn Elisa Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar COLs, immunostaining of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.

Journal: Advanced therapeutics

Article Title: Inhibition of Collagenase Q1 of Bacillus cereus as a Novel Antivirulence Strategy for the Treatment of Skin-Wound Infections

doi: 10.1002/adtp.202100222

Figure Lengend Snippet: a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar COLs, immunostaining of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.

Article Snippet: For immunostaining, tissue samples were stained with primary antibodies. (COL I (Rabbit polyclonal anti-type I collagen, (600-401-103-0.1 Rockland); COL III (rabbit anti human collagen III antibody, (Abcam, ab7778)); COL V (rabbit anti human collagen V antibody, (Abcam, ab7046)) (1:200 dilutions in PBS) at RT for 1 h or at 4 °C ON.

Techniques: Immunostaining, Standard Deviation

a) Chemical structures of compounds 1 and 2 and the calculated IC 50 value in the FRET-based ColQ1, ColH, and LasB inhibition assay. b) Effect of ColQ1 inhibitors on the cleavage of COL I after challenge with 50 ng of ColQ1. c,d) Effect of compounds 1 and 2 on B . cereus csn monitored by c) gelatin zymography. The gelatin-degradation assay was performed in the presence of inhibitors or the buffer control. Due to limited solubility in the reaction buffer, compound 2 could only be tested at 100 × 10 −6 m compared to 300 × 10 −6 m of compound 1 . d) Densitometric analysis of gelatin zymography shown in (c). Image analysis was performed with Image Studio Lite v5.2 software (Li-Cor Biosciences, USA). B . cereus : Bacillus cereus , csn: culture supernatant, COL: collagen, n.d.: not determined.

Journal: Advanced therapeutics

Article Title: Inhibition of Collagenase Q1 of Bacillus cereus as a Novel Antivirulence Strategy for the Treatment of Skin-Wound Infections

doi: 10.1002/adtp.202100222

Figure Lengend Snippet: a) Chemical structures of compounds 1 and 2 and the calculated IC 50 value in the FRET-based ColQ1, ColH, and LasB inhibition assay. b) Effect of ColQ1 inhibitors on the cleavage of COL I after challenge with 50 ng of ColQ1. c,d) Effect of compounds 1 and 2 on B . cereus csn monitored by c) gelatin zymography. The gelatin-degradation assay was performed in the presence of inhibitors or the buffer control. Due to limited solubility in the reaction buffer, compound 2 could only be tested at 100 × 10 −6 m compared to 300 × 10 −6 m of compound 1 . d) Densitometric analysis of gelatin zymography shown in (c). Image analysis was performed with Image Studio Lite v5.2 software (Li-Cor Biosciences, USA). B . cereus : Bacillus cereus , csn: culture supernatant, COL: collagen, n.d.: not determined.

Article Snippet: For immunostaining, tissue samples were stained with primary antibodies. (COL I (Rabbit polyclonal anti-type I collagen, (600-401-103-0.1 Rockland); COL III (rabbit anti human collagen III antibody, (Abcam, ab7778)); COL V (rabbit anti human collagen V antibody, (Abcam, ab7046)) (1:200 dilutions in PBS) at RT for 1 h or at 4 °C ON.

Techniques: Inhibition, Zymography, Degradation Assay, Solubility, Software

In vitro biological activity test of IWBLHs. (A) The cell toxicity result of extract of various IWBLHs. (B) The BMSCs adhesion results of IWBLHs. (C) BMSCs proliferation result of IWBLHs. (D) ALP and (E) OCN of BMSCs growing on various IWBLHs, the weight ratio indicated SA: ACP: mineralized collagen fibril ( n = 3, experiments performed in triplicate. The bars show the mean ± SD, * p < 0.05).

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: The Injectable Woven Bone-Like Hydrogel to Perform Alveolar Ridge Preservation With Adapted Remodeling Performance After Tooth Extraction

doi: 10.3389/fbioe.2020.00119

Figure Lengend Snippet: In vitro biological activity test of IWBLHs. (A) The cell toxicity result of extract of various IWBLHs. (B) The BMSCs adhesion results of IWBLHs. (C) BMSCs proliferation result of IWBLHs. (D) ALP and (E) OCN of BMSCs growing on various IWBLHs, the weight ratio indicated SA: ACP: mineralized collagen fibril ( n = 3, experiments performed in triplicate. The bars show the mean ± SD, * p < 0.05).

Article Snippet: At the predetermined time, the alkaline phosphatase (ALP) and osteocalcin (OCN) of cells were tested using the ALP assay kit (Beyotime Institute of Biotechnology, China) and OCN ELISA kit (Nanjing Jiancheng Bioengineering Institute, China), respectively.

Techniques: In Vitro, Activity Assay