Journal: Molecular Metabolism

Article Title: Aregs-IGFBP3-mediated SMC-like cells apoptosis impairs beige adipocytes formation in aged mice

doi: 10.1016/j.molmet.2025.102125

Figure Lengend Snippet: Aging induces an elevation in Aregs and a reduction in SMC-like cells among ASPCs following cold exposure . (A) UMAP plot showing the clustering distribution of 58,714 cells from young and aged mice sWAT. (B) Dot plot displaying the expression levels and proportions of selected marker genes across different cell types. The size of the dot represents the percentage of cells expressing the gene, while the color intensity reflects the average gene expression level. (C) Stacked bar chart showing the proportion of cell types in sWAT at different time points (TN, 3 days, and 14 days post-cold exposure). (D) Ucp1 and Cd142 mRNA expression levels analyzed by qPCR in sWAT (n = 11). (E) Immunoblots for CD142, UCP1, TOMM20 and GAPDH in sWAT (n = 3). (F) Quantification of immunoblots of CD142, UCP1 and TOMM20, protein levels are normalized to GAPDH. (G) Immunohistochemistry fluorescence for UCP1 (red) and CD142 (green) (scale bar = 50 μm or 10 μm). Data represent mean ± SD, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Next, adipocytes were subjected to an overnight incubation at 4 °C with CD142 primary antibody (1:100, R&D Systems, AF3178).

Techniques: Expressing, Marker, Gene Expression, Western Blot, Immunohistochemistry, Fluorescence

Journal: Molecular Metabolism

Article Title: Aregs-IGFBP3-mediated SMC-like cells apoptosis impairs beige adipocytes formation in aged mice

doi: 10.1016/j.molmet.2025.102125

Figure Lengend Snippet: Aregs and IGFBP3 inhibit beige adipocyte formation. (A–E) All five groups of ASPCs underwent the same browning differentiation protocol. (A) Ucp1 mRNA expression levels analyzed by qPCR. (B) Immunoblots for UCP1, TOMM20 and GAPDH. (C,D) Quantification of immunoblots of UCP1 and TOMM20, protein levels are normalized to GAPDH. (E) Immunofluorescence staining for CD142 and Mitotracker for mitochondria (scale bar = 20 μm). Data represent mean ± SD, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Next, adipocytes were subjected to an overnight incubation at 4 °C with CD142 primary antibody (1:100, R&D Systems, AF3178).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining