cntl Search Results


ucsd246i-cntl-2  (WiCell Research Institute Inc)
N/A
This iPS cell line is an isogenic control for Long QT Syndrome iPS cell lines available from WiCell. It has undergone the LQT-causal variant gene editing pathway but is unmodified. Additional clone (UCSD245i-CNTL-1) is available
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cntl-sirna  (Ribobio co)
90
Ribobio co cntl-sirna
Cntl Sirna, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cntl/cntl+sirna/pmc07874639-119-10-11
Average 90 stars, based on 1 article reviews
cntl-sirna - by Bioz Stars, 2026-08
90/100 stars
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cntl  (Microsynth ag)
90
Microsynth ag cntl
( a ) U2OS Flp-In T-REx cells stably expressing doxycycline (Dox)-inducible GFP-KLHL15-wt were cultivated in the absence (−) or presence (+) of Dox. Twenty-four hours post induction, cells were either mock-treated or treated with MG-132 (10 μM) for 6 h and whole-cell lysates were analysed by immunoblotting. ( b ) Twenty-four hours post induction, same cells as in a were either mock-treated or treated with MLN-4924 (100 nM) for 8 h and whole-cell lysates were analysed by immunoblotting. ( c ) Same cells as in a were transfected with either non-targeting <t>(CNTL)</t> or CUL3 siRNA oligos. 48 h later, GFP-KLHL15 expression was induced with Dox for 24 h and whole-cell lysates were analysed by immunoblotting. ( d ) U2OS cells were transfected with the indicated siRNA oligos for 48 h. Total cell extracts and chromatin-enriched fractions were analysed by immunoblotting using the indicated antibodies. ( e ) Same cells as in a were transfected with the indicated siRNA oligos. Twenty-four hours post transfection, cells were grown for 24 h in the absence (−) or presence (+) of Dox and whole-cell lysates were analysed by immunoblotting. ( f ) HEK293 Flp-In T-REx cells inducibly <t>expressing</t> <t>GFP-CtIP</t> were transfected with the indicated siRNAs. Forty-eight hours later, cells were transfected with His-Ub and the expression of CtIP was simultaneously induced with Dox. Eight hours post induction, cells were transfected with siRNAs for a second time. Three days after the first siRNA transfection, cells were treated with MG-132 (20 μM) for 6 h followed by lysis in buffer containing guanidium-HCl. Ubiquitin conjugates were pulled-down (PD) with Ni-NTA-agarose beads, eluted and analysed by immunoblotting with anti-GFP antibody. ( g ) Recombinant FLAG-CtIP was incubated with ATP, ubiquitin, E1, E2 enzymes and CUL3-N8/RBX1-KLHL15 (E3) for 30 min at 37 °C. Unmodified and ubiquitinated CtIP protein species were detected by immunoblotting with anti-FLAG antibody. Asterisks indicate neddylated CUL3.
Cntl, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cntl/cntl/pmc05007465-219-18-8
Average 90 stars, based on 1 article reviews
cntl - by Bioz Stars, 2026-08
90/100 stars
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normal chow diet cntl d20122207  (research diets inc)
90
research diets inc normal chow diet cntl d20122207
( a ) U2OS Flp-In T-REx cells stably expressing doxycycline (Dox)-inducible GFP-KLHL15-wt were cultivated in the absence (−) or presence (+) of Dox. Twenty-four hours post induction, cells were either mock-treated or treated with MG-132 (10 μM) for 6 h and whole-cell lysates were analysed by immunoblotting. ( b ) Twenty-four hours post induction, same cells as in a were either mock-treated or treated with MLN-4924 (100 nM) for 8 h and whole-cell lysates were analysed by immunoblotting. ( c ) Same cells as in a were transfected with either non-targeting <t>(CNTL)</t> or CUL3 siRNA oligos. 48 h later, GFP-KLHL15 expression was induced with Dox for 24 h and whole-cell lysates were analysed by immunoblotting. ( d ) U2OS cells were transfected with the indicated siRNA oligos for 48 h. Total cell extracts and chromatin-enriched fractions were analysed by immunoblotting using the indicated antibodies. ( e ) Same cells as in a were transfected with the indicated siRNA oligos. Twenty-four hours post transfection, cells were grown for 24 h in the absence (−) or presence (+) of Dox and whole-cell lysates were analysed by immunoblotting. ( f ) HEK293 Flp-In T-REx cells inducibly <t>expressing</t> <t>GFP-CtIP</t> were transfected with the indicated siRNAs. Forty-eight hours later, cells were transfected with His-Ub and the expression of CtIP was simultaneously induced with Dox. Eight hours post induction, cells were transfected with siRNAs for a second time. Three days after the first siRNA transfection, cells were treated with MG-132 (20 μM) for 6 h followed by lysis in buffer containing guanidium-HCl. Ubiquitin conjugates were pulled-down (PD) with Ni-NTA-agarose beads, eluted and analysed by immunoblotting with anti-GFP antibody. ( g ) Recombinant FLAG-CtIP was incubated with ATP, ubiquitin, E1, E2 enzymes and CUL3-N8/RBX1-KLHL15 (E3) for 30 min at 37 °C. Unmodified and ubiquitinated CtIP protein species were detected by immunoblotting with anti-FLAG antibody. Asterisks indicate neddylated CUL3.
Normal Chow Diet Cntl D20122207, supplied by research diets inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cntl/normal+chow+diet+cntl+d20122207/pmc10859874-133-14-19
Average 90 stars, based on 1 article reviews
normal chow diet cntl d20122207 - by Bioz Stars, 2026-08
90/100 stars
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human chi3l1  (R&D Systems)
92
R&D Systems human chi3l1
( a ) U2OS Flp-In T-REx cells stably expressing doxycycline (Dox)-inducible GFP-KLHL15-wt were cultivated in the absence (−) or presence (+) of Dox. Twenty-four hours post induction, cells were either mock-treated or treated with MG-132 (10 μM) for 6 h and whole-cell lysates were analysed by immunoblotting. ( b ) Twenty-four hours post induction, same cells as in a were either mock-treated or treated with MLN-4924 (100 nM) for 8 h and whole-cell lysates were analysed by immunoblotting. ( c ) Same cells as in a were transfected with either non-targeting <t>(CNTL)</t> or CUL3 siRNA oligos. 48 h later, GFP-KLHL15 expression was induced with Dox for 24 h and whole-cell lysates were analysed by immunoblotting. ( d ) U2OS cells were transfected with the indicated siRNA oligos for 48 h. Total cell extracts and chromatin-enriched fractions were analysed by immunoblotting using the indicated antibodies. ( e ) Same cells as in a were transfected with the indicated siRNA oligos. Twenty-four hours post transfection, cells were grown for 24 h in the absence (−) or presence (+) of Dox and whole-cell lysates were analysed by immunoblotting. ( f ) HEK293 Flp-In T-REx cells inducibly <t>expressing</t> <t>GFP-CtIP</t> were transfected with the indicated siRNAs. Forty-eight hours later, cells were transfected with His-Ub and the expression of CtIP was simultaneously induced with Dox. Eight hours post induction, cells were transfected with siRNAs for a second time. Three days after the first siRNA transfection, cells were treated with MG-132 (20 μM) for 6 h followed by lysis in buffer containing guanidium-HCl. Ubiquitin conjugates were pulled-down (PD) with Ni-NTA-agarose beads, eluted and analysed by immunoblotting with anti-GFP antibody. ( g ) Recombinant FLAG-CtIP was incubated with ATP, ubiquitin, E1, E2 enzymes and CUL3-N8/RBX1-KLHL15 (E3) for 30 min at 37 °C. Unmodified and ubiquitinated CtIP protein species were detected by immunoblotting with anti-FLAG antibody. Asterisks indicate neddylated CUL3.
Human Chi3l1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cntl/Quantikine+Immunoassay+Cntl+Set+1027+Human+CHI3L1%2FYKL-40/pm38663404-630-12-14
Average 92 stars, based on 1 article reviews
human chi3l1 - by Bioz Stars, 2026-08
92/100 stars
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quantikine human chi3l1 immunoassay  (R&D Systems)
93
R&D Systems quantikine human chi3l1 immunoassay
( a ) U2OS Flp-In T-REx cells stably expressing doxycycline (Dox)-inducible GFP-KLHL15-wt were cultivated in the absence (−) or presence (+) of Dox. Twenty-four hours post induction, cells were either mock-treated or treated with MG-132 (10 μM) for 6 h and whole-cell lysates were analysed by immunoblotting. ( b ) Twenty-four hours post induction, same cells as in a were either mock-treated or treated with MLN-4924 (100 nM) for 8 h and whole-cell lysates were analysed by immunoblotting. ( c ) Same cells as in a were transfected with either non-targeting <t>(CNTL)</t> or CUL3 siRNA oligos. 48 h later, GFP-KLHL15 expression was induced with Dox for 24 h and whole-cell lysates were analysed by immunoblotting. ( d ) U2OS cells were transfected with the indicated siRNA oligos for 48 h. Total cell extracts and chromatin-enriched fractions were analysed by immunoblotting using the indicated antibodies. ( e ) Same cells as in a were transfected with the indicated siRNA oligos. Twenty-four hours post transfection, cells were grown for 24 h in the absence (−) or presence (+) of Dox and whole-cell lysates were analysed by immunoblotting. ( f ) HEK293 Flp-In T-REx cells inducibly <t>expressing</t> <t>GFP-CtIP</t> were transfected with the indicated siRNAs. Forty-eight hours later, cells were transfected with His-Ub and the expression of CtIP was simultaneously induced with Dox. Eight hours post induction, cells were transfected with siRNAs for a second time. Three days after the first siRNA transfection, cells were treated with MG-132 (20 μM) for 6 h followed by lysis in buffer containing guanidium-HCl. Ubiquitin conjugates were pulled-down (PD) with Ni-NTA-agarose beads, eluted and analysed by immunoblotting with anti-GFP antibody. ( g ) Recombinant FLAG-CtIP was incubated with ATP, ubiquitin, E1, E2 enzymes and CUL3-N8/RBX1-KLHL15 (E3) for 30 min at 37 °C. Unmodified and ubiquitinated CtIP protein species were detected by immunoblotting with anti-FLAG antibody. Asterisks indicate neddylated CUL3.
Quantikine Human Chi3l1 Immunoassay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cntl/Quantikine+Immunoassay+Cntl+Set+1027+Human+CHI3L1%2FYKL-40/pm25740558-74-7-11
Average 93 stars, based on 1 article reviews
quantikine human chi3l1 immunoassay - by Bioz Stars, 2026-08
93/100 stars
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percp cy5 5 rat igg1 iso cntl hrpn 100ug  (Tonbo Biosciences)
N/A
PerCP Cy5 5 Rat IgG2b Isotype Control HRPN
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ucsd245i-cntl-1  (WiCell Research Institute Inc)
N/A
This iPS cell line is an isogenic control for Long QT Syndrome iPS cell lines available from WiCell. It has undergone the LQT-causal variant gene editing pathway but is unmodified. Additional clone (UCSD246i-CNTL-2) is available
   Buy from Supplier

Image Search Results


( a ) U2OS Flp-In T-REx cells stably expressing doxycycline (Dox)-inducible GFP-KLHL15-wt were cultivated in the absence (−) or presence (+) of Dox. Twenty-four hours post induction, cells were either mock-treated or treated with MG-132 (10 μM) for 6 h and whole-cell lysates were analysed by immunoblotting. ( b ) Twenty-four hours post induction, same cells as in a were either mock-treated or treated with MLN-4924 (100 nM) for 8 h and whole-cell lysates were analysed by immunoblotting. ( c ) Same cells as in a were transfected with either non-targeting (CNTL) or CUL3 siRNA oligos. 48 h later, GFP-KLHL15 expression was induced with Dox for 24 h and whole-cell lysates were analysed by immunoblotting. ( d ) U2OS cells were transfected with the indicated siRNA oligos for 48 h. Total cell extracts and chromatin-enriched fractions were analysed by immunoblotting using the indicated antibodies. ( e ) Same cells as in a were transfected with the indicated siRNA oligos. Twenty-four hours post transfection, cells were grown for 24 h in the absence (−) or presence (+) of Dox and whole-cell lysates were analysed by immunoblotting. ( f ) HEK293 Flp-In T-REx cells inducibly expressing GFP-CtIP were transfected with the indicated siRNAs. Forty-eight hours later, cells were transfected with His-Ub and the expression of CtIP was simultaneously induced with Dox. Eight hours post induction, cells were transfected with siRNAs for a second time. Three days after the first siRNA transfection, cells were treated with MG-132 (20 μM) for 6 h followed by lysis in buffer containing guanidium-HCl. Ubiquitin conjugates were pulled-down (PD) with Ni-NTA-agarose beads, eluted and analysed by immunoblotting with anti-GFP antibody. ( g ) Recombinant FLAG-CtIP was incubated with ATP, ubiquitin, E1, E2 enzymes and CUL3-N8/RBX1-KLHL15 (E3) for 30 min at 37 °C. Unmodified and ubiquitinated CtIP protein species were detected by immunoblotting with anti-FLAG antibody. Asterisks indicate neddylated CUL3.

Journal: Nature Communications

Article Title: Cullin3-KLHL15 ubiquitin ligase mediates CtIP protein turnover to fine-tune DNA-end resection

doi: 10.1038/ncomms12628

Figure Lengend Snippet: ( a ) U2OS Flp-In T-REx cells stably expressing doxycycline (Dox)-inducible GFP-KLHL15-wt were cultivated in the absence (−) or presence (+) of Dox. Twenty-four hours post induction, cells were either mock-treated or treated with MG-132 (10 μM) for 6 h and whole-cell lysates were analysed by immunoblotting. ( b ) Twenty-four hours post induction, same cells as in a were either mock-treated or treated with MLN-4924 (100 nM) for 8 h and whole-cell lysates were analysed by immunoblotting. ( c ) Same cells as in a were transfected with either non-targeting (CNTL) or CUL3 siRNA oligos. 48 h later, GFP-KLHL15 expression was induced with Dox for 24 h and whole-cell lysates were analysed by immunoblotting. ( d ) U2OS cells were transfected with the indicated siRNA oligos for 48 h. Total cell extracts and chromatin-enriched fractions were analysed by immunoblotting using the indicated antibodies. ( e ) Same cells as in a were transfected with the indicated siRNA oligos. Twenty-four hours post transfection, cells were grown for 24 h in the absence (−) or presence (+) of Dox and whole-cell lysates were analysed by immunoblotting. ( f ) HEK293 Flp-In T-REx cells inducibly expressing GFP-CtIP were transfected with the indicated siRNAs. Forty-eight hours later, cells were transfected with His-Ub and the expression of CtIP was simultaneously induced with Dox. Eight hours post induction, cells were transfected with siRNAs for a second time. Three days after the first siRNA transfection, cells were treated with MG-132 (20 μM) for 6 h followed by lysis in buffer containing guanidium-HCl. Ubiquitin conjugates were pulled-down (PD) with Ni-NTA-agarose beads, eluted and analysed by immunoblotting with anti-GFP antibody. ( g ) Recombinant FLAG-CtIP was incubated with ATP, ubiquitin, E1, E2 enzymes and CUL3-N8/RBX1-KLHL15 (E3) for 30 min at 37 °C. Unmodified and ubiquitinated CtIP protein species were detected by immunoblotting with anti-FLAG antibody. Asterisks indicate neddylated CUL3.

Article Snippet: CNTL, CtIP, CUL3 and KLHL15#2 were purchased from Microsynth and the sequences (5′ to 3′) were as follows: CNTL (luciferase; 5′-CGUACGCGGAAUACUUCGA-3′) , CtIP (5′-GCUAAAACAGGAACGAAUC-3′) , CUL3 (5′-CAACACTTGGCAAGGAGAC-3′) and KLHL15#2 (5′-GCGTAAACATCGAGGGAG-3′).

Techniques: Stable Transfection, Expressing, Western Blot, Transfection, Lysis, Ubiquitin Proteomics, Recombinant, Incubation