Journal: Oncotarget

Article Title: Deregulation of focal adhesion pathway mediated by miR-659-3p is implicated in bone marrow infiltration of stage M neuroblastoma patients

doi:

Figure Lengend Snippet: Genes specifically modulated by treatment with miR-659-3p mimic, known to be differentially expressed by BM-infiltrating cells and primary tumors

Article Snippet: To evaluate gene expression levels in miR-659-3p transfected cells, 150 ng of total RNA was reverse transcribed as described [ , ], and then amplified in triplicate with the specific TaqMan © human gene expression assay ( CNOT1 : Hs00406740_m1, GTSE1 : Hs00212681_m1, KIF4A : Hs00602211_g1, AKT3 : Hs00987350_m1, BCL2 : Hs00608023_m1, CYR61 : Hs00998500_g1, HEY1 : Hs01114113_m1, RNASEL : Hs00221692_m1, THBS2 : Hs01568063_m1, ZNF652: Hs00977533_m1), and for HPRT1 (Hs99999909_m1) as endogenous reference gene (Life Technologies).

Techniques:

Journal: BMC Medical Genomics

Article Title: Genetic analysis of partial duplication of the long arm of chromosome 16

doi: 10.1186/s12920-024-02059-3

Figure Lengend Snippet: Genotypes and phenotypes associated with patient’s duplication in the OMIM database

Article Snippet: The GNAO1 , CNOT1 , and CTCF genes, as documented in OMIM/Morbid, the Gene Curation Coalition, Gene2Phenotype (G2P), and ClinGen, were classified as having definitive pathogenicity, associated with autosomal dominant (AD) inheritance.

Techniques: Gene Expression, Scaffolding, Binding Assay, Ubiquitin Proteomics, Activation Assay