Journal: Cell Genomics

Article Title: A cell and transcriptome atlas of human arterial vasculature

doi: 10.1016/j.xgen.2025.101034

Figure Lengend Snippet: Smooth muscle cells of great arteries (A) Featureplots demonstrating distinct distributions and specificity of contractile SMC markers. (B) Immunofluorescence (IF) staining for CNN1 (green) of coronary tissue showing medial and adventitial (Adv)population. (C) Featureplot of RGS5 , a commonly used pericyte marker delineating multiple populations. (D) Differentially expressed genes (DEGs) ranking between the two major smooth muscle cell clusters. (E) Dot plot summarizing Gene Ontology (GO) analysis of DEGs between the two clusters containing SMCs. (F) Featureplot and RNAscope of microvascular specific marker. (G) Featureplot of intimal/medial SMC markers demonstrating spatial or functional heterogeneity among SMC subtypes. (H) Spatial transcriptomics maps and RNAscope demonstrating distinct spatial localization intimal/medial SMC clusters/markers from (G). See also and .

Article Snippet: CNN1 , OriGene , SKU: TA327614; RRID: AB_3716482.

Techniques: Immunofluorescence, Staining, Marker, RNAscope, Functional Assay

Journal: Nature Communications

Article Title: In vitro fabrication of functional three-dimensional tissues with perfusable blood vessels

doi: 10.1038/ncomms2406

Figure Lengend Snippet: ( a ) The six-layer tissue graft engineered by the double-step procedure was removed from the bioreactor system and transplanted in the neck of a nude rat with anastomosis between the protruding femoral vessels of the graft and the connectable neck blood vessels of the host rat. ( b ) The six-layer graft was transplanted without the vessel anastomosis. ( c ) The six-layer cell sheets (CS) without the vascular bed (VB) were simply transplanted. ( d – f ) In vivo bioluminescent imaging of each graft is shown. Obvious signals were observed in the graft with vessel anastomoses ( d ). The intensity was low in the graft without anastomosis ( e ) and in the graft without a vascular bed ( f ). ( g ) The intensity was significantly the highest in the graft with vessel anastomosis, compared with the other two grafts. Data are shown as mean±s.d. (* P <0.05) ( n =4). ( h ) The vascularized graft with blood vessel anastomoses also maintained its vascular structure at 2 weeks after the procedure. Dotted line indicates the area of the graft with the vascular bed and arrows indicate the area of the cell sheet layers. ( i ) Immunostaining for CD31 (green: ECs) and calponin (red: smooth muscle cell) shows the uniform distribution of micro blood vessels in the cell sheet construct. ( j ) The detail in the white box of i illustrates the characteristics of a matured blood vessels including red blood cells.

Article Snippet: For the detection of cardiomyocytes, sections were incubated with a 1:200 dilution of anti-sarcomeric α-actinin mouse monoclonal antibody (Sigma, St Louis, MO) for 2 h. For the detection of smooth muscle cells, sections were incubated with a 1/100 dilution of anti-calponin mouse monoclonal antibody (Acris Antibodies, Herford, Germany).

Techniques: In Vivo, Imaging, Immunostaining, Construct