Journal: PLoS Genetics

Article Title: Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes

doi: 10.1371/journal.pgen.1005605

Figure Lengend Snippet: Prometaphase karyosome configurations in the absence of kinetochore components.

Article Snippet: The qPCR was performed in a StepOnePlus (Life Technologies) real-time PCR system using TaqMan Gene Expression Assays (Life Technologies, Dm01843531_g1 for cmet , Dm01838612_g1 for Ndc80 , Dm01792082_g1 for Spc105R , and Dm02134593_g1 for Rpll140 as control).

Techniques:

Journal: PLoS Genetics

Article Title: Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes

doi: 10.1371/journal.pgen.1005605

Figure Lengend Snippet: Confocal images of FISH probes marking the 2 nd (red) and 3 rd (white) chromosome centromeres. DNA is in blue and tubulin is in green in merged images. Only FISH probes are shown in the panel below each merged image. Scale bars represent 10 μm. (A) Oocytes from wild type and after knockdown of Ndc80 or Spc105R . (B) Dot plot of the distance between pairs of FISH foci in wild-type oocytes and after knockdown of Ndc80 or Spc105R . Horizontal dotted lines show the mean, error bars show 95% confidence intervals. (C) Oocytes from cana/Df , after knockdown of cmet , and Cenp-E germline clones. The data is summarized in .

Article Snippet: The qPCR was performed in a StepOnePlus (Life Technologies) real-time PCR system using TaqMan Gene Expression Assays (Life Technologies, Dm01843531_g1 for cmet , Dm01838612_g1 for Ndc80 , Dm01792082_g1 for Spc105R , and Dm02134593_g1 for Rpll140 as control).

Techniques: Knockdown, Clone Assay

Journal: PLoS Genetics

Article Title: Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes

doi: 10.1371/journal.pgen.1005605

Figure Lengend Snippet: Confocal images of karyosome and spindle organization in oocytes from wild type, cana/Df , after knockdown of cmet , Cenp-E germline clones, and after knockdown of Ndc80 , Spc105R (HMS01548), Ndc80 and cmet , or Spc105R (HMS01548) and cmet . DNA is in blue and tubulin is in green in merged images. Single channel images show DNA in white. Scale bars represent 10 μm.

Article Snippet: The qPCR was performed in a StepOnePlus (Life Technologies) real-time PCR system using TaqMan Gene Expression Assays (Life Technologies, Dm01843531_g1 for cmet , Dm01838612_g1 for Ndc80 , Dm01792082_g1 for Spc105R , and Dm02134593_g1 for Rpll140 as control).

Techniques: Knockdown, Clone Assay

Journal: PLoS Genetics

Article Title: Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes

doi: 10.1371/journal.pgen.1005605

Figure Lengend Snippet: Centromere bi-orientation in the absence of CANA and CMET.

Article Snippet: The qPCR was performed in a StepOnePlus (Life Technologies) real-time PCR system using TaqMan Gene Expression Assays (Life Technologies, Dm01843531_g1 for cmet , Dm01838612_g1 for Ndc80 , Dm01792082_g1 for Spc105R , and Dm02134593_g1 for Rpll140 as control).

Techniques:

Journal: Molecular Oncology

Article Title: Volitinib, a potent and highly selective c‐Met inhibitor, effectively blocks c‐Met signaling and growth in c‐MET amplified gastric cancer patient‐derived tumor xenograft models ‡

doi: 10.1016/j.molonc.2014.08.015

Figure Lengend Snippet: Potent volitinib in vitro anti‐proliferative activity correlates with dysregulation of cMET expression and pharmacodynamic activity in a gastric cancer cell line panel screen. A, 22 gastric cancer cell lines were tabulated alongside the ...

Article Snippet: The cMET FISH probe was generated internally by directly labeling BAC (CTD‐2270N20) DNA with Spectrum Red (ENZO, Cat # 02N34‐050).

Techniques: In Vitro, Activity Assay, Expressing

Journal: Molecular Oncology

Article Title: Volitinib, a potent and highly selective c‐Met inhibitor, effectively blocks c‐Met signaling and growth in c‐MET amplified gastric cancer patient‐derived tumor xenograft models ‡

doi: 10.1016/j.molonc.2014.08.015

Figure Lengend Snippet: Volitinib treatment results in anti‐tumor efficacy and pharmacodynamic modulation of cMET signaling in the cMET‐driven xenograft model, Hs746t. A, Volitinib was administered by oral gavage once daily (qd) to nu/nu mice bearing established ...

Article Snippet: The cMET FISH probe was generated internally by directly labeling BAC (CTD‐2270N20) DNA with Spectrum Red (ENZO, Cat # 02N34‐050).

Techniques:

Journal: Molecular Oncology

Article Title: Volitinib, a potent and highly selective c‐Met inhibitor, effectively blocks c‐Met signaling and growth in c‐MET amplified gastric cancer patient‐derived tumor xenograft models ‡

doi: 10.1016/j.molonc.2014.08.015

Figure Lengend Snippet: Volitinib displays antitumor efficacy in short duration efficacy studies using cMET‐dysregulated gastric cancer patient‐derived xenograft models. A, cMET gene and protein expression levels were characterized in all 4 gastric PDX models ...

Article Snippet: The cMET FISH probe was generated internally by directly labeling BAC (CTD‐2270N20) DNA with Spectrum Red (ENZO, Cat # 02N34‐050).

Techniques: Derivative Assay, Expressing

Journal: Molecular Oncology

Article Title: Volitinib, a potent and highly selective c‐Met inhibitor, effectively blocks c‐Met signaling and growth in c‐MET amplified gastric cancer patient‐derived tumor xenograft models ‡

doi: 10.1016/j.molonc.2014.08.015

Figure Lengend Snippet: Volitinib enhances the efficacy of docetaxel in both standard and patient‐derived cMET‐driven xenograft models. A, Volitinib, docetaxel or a combination of the two were administered according to the administration routes and schedules ...

Article Snippet: The cMET FISH probe was generated internally by directly labeling BAC (CTD‐2270N20) DNA with Spectrum Red (ENZO, Cat # 02N34‐050).

Techniques: Derivative Assay