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  • 99
    Vector Laboratories vectastain abc kit
    Localization of bovine 20α-HSD protein expression in the CL throughout the estrous cycle by immunohistochemistry. Representative immunohistochemical analyses using 20α-HSD antiserum (1:1000) and goat anti-rabbit secondary antibodies (1:500). Preimmune serum (1:1000) was used for primary antiserum as the negative control. Immunohistochemistry was performed by a <t>Vectastain</t> <t>ABC</t> kit. Ovarian sections are shown during the estrous cycle. Preimmune serum was used as the control for the CL stage 7 below the right panel. Black bar=100 μm. Red arrows indicate luteal cells.
    Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 26234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC hela cells
    Inhibition of <t>CVB3</t> replication by PDTC is independent of its antioxidant activity. (A) <t>HeLa</t> cells were sham infected with PBS or infected with CVB3 (MOI = 10) in the presence of various inhibitors. Redox-sensitive fluorescence probe CM-H 2 DCFDA
    Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dapi
    PNx induces increased nuclei numbers in α 1 +/− mice. Left ventricle sections from each experimental group were stained with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI;</t> blue) and cardiac <t>troponin</t> I (cTnI, red) as described in materials
    Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 155801 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trypsin edta
    Anti-rotaviral activity of RV6-26. (A) Inhibition of in vitro transcription: <t>EDTA-activated</t> DLP were incubated with 200 nM combining sites of different antibodies and mRNA was synthesized in vitro using selected components of the Riboprobe SP6 system (transcription was mediated by the viral RNA-dependent RNA polymerase not the SP6 DNA-dependent RNA polymerase). First-strand cDNA was synthesized by reverse transcription using a VP6-specific primer. Amplification of cDNA with VP6-specific primers was monitored in a real-time PCR using SYBR Green; the concentrations of RNA estimated from a standard curve constructed using reference RNA extracted from RRV are plotted. (B) Inhibition of <t>rotavirus</t> replication by IgA: polarized monolayers of Caco-2 cells grown on Transwell inserts were treated with polymeric IgA in the basal compartment and inoculated apically with trypsin-activated RRV (MOI = 5) at ambient temperature for 1 h and then cultured for 16 in medium containing trypsin. Amount of rotavirus in the inserts was titrated by inoculating MA104 cells and culturing for 16 h, followed by acetone-fixation and staining with anti-rotavirus polyclonal antibodies conjugated to either Alexa568 or IRDye 800. Detection was done either by scanning on Licor or by counting fluorescent foci.
    Trypsin Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 49323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fbs
    Effects of glyceollin I on colony formation on MCF-7 and BG-1 cells. MCF-7 (A) and BG-1 (B) cells were placed in phenol red-free <t>DMEM</t> supplemented with 5% dextran-coated charcoal-treated <t>FBS</t> for 48 h before plating. MCF-7 or BG-1 cells (1000) were plated
    Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 73394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad immun blot pvdf membrane
    Immunoblot analysis of purified C. difficile toxins A and B. Purified proteins (80 µg each) were subjected to 6% <t>PAGE</t> and transferred onto <t>PVDF</t> membranes. Each membrane was probed using monoclonal primary antibodies specific for toxin A or B. The Pierce ECL Western Blotting Kit was used to detect the bound antibodies. The membrane was exposed to X-ray film (Molecular Technologies, St Louis, MO) and processed using a Konica film processor (Konica Corporation, Tokyo, Japan). Sup, crude culture supernatant; Toxin A, purified toxin A; Toxin B, purified toxin B.
    Immun Blot Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dmem
    Decahedral AgNPs were highly uniform. (a) High-resolution scanning electron microscopy image of AgNPs. Scale bar: 200 nm. (b) High-resolution transmission electron microscopy image of AgNPs. Scale bar: 100 nm. (c) Dynamic light scattering for characterization of particles’ size and distribution in water and complete medium. (d) Positive linear relationship between different concentrations of AgNPs in <t>DMEM</t> containing 10% (v/v) <t>FBS</t> and 1% (v/v) P–S and the corresponding peak absorbance intensity at 495 nm.
    Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 147955 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher l glutamine
    Decahedral AgNPs were highly uniform. (a) High-resolution scanning electron microscopy image of AgNPs. Scale bar: 200 nm. (b) High-resolution transmission electron microscopy image of AgNPs. Scale bar: 100 nm. (c) Dynamic light scattering for characterization of particles’ size and distribution in water and complete medium. (d) Positive linear relationship between different concentrations of AgNPs in <t>DMEM</t> containing 10% (v/v) <t>FBS</t> and 1% (v/v) P–S and the corresponding peak absorbance intensity at 495 nm.
    L Glutamine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 146399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher penicillin streptomycin
    Decahedral AgNPs were highly uniform. (a) High-resolution scanning electron microscopy image of AgNPs. Scale bar: 200 nm. (b) High-resolution transmission electron microscopy image of AgNPs. Scale bar: 100 nm. (c) Dynamic light scattering for characterization of particles’ size and distribution in water and complete medium. (d) Positive linear relationship between different concentrations of AgNPs in <t>DMEM</t> containing 10% (v/v) <t>FBS</t> and 1% (v/v) P–S and the corresponding peak absorbance intensity at 495 nm.
    Penicillin Streptomycin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 156248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore hepes
    SANS data analysis of MtrCAB in 20 m m <t>HEPES</t> pH 7. 8, 100 m m <t>NaCl,</t> 13% D 2 O, 2.8 m m Fos-choline-12, at 3.5 ( black ) and 9.7 ( gray ) mg/ml. A , Guinier region of MtrCAB with linear fit to data shown suggesting an R g of 54 Å. Residuals of fit are shown above. B , Kratky plot of scaled MtrCAB suggests slight differences between the data at 3.5 and 9.7 mg/ml. C and D , scattering curves with fits to the data giving rise to the P ( r ) distance distribution curves shown in ( D ).
    Hepes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 50050 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher trizol reagent
    MHV-ExoN(-) evolved WT-like genomic <t>RNA</t> accumulation and increased resistance to multiple nucleoside analogues over the passage. (A) Cells were infected with the indicated viruses at MOI = 1 PFU/cell, and intracellular RNA was harvested using <t>TRIzol</t> at the indicated times postinfection. MHV genomic RNA was detected using SYBR green and primers directed to nsp10, and values were normalized to intracellular GAPDH. (B) Same data as in panel A normalized to the RNA level for each virus at 4 hpi. Data represent means and standard errors of results for n = 9 (3 triplicate experiments). (C to F) Sensitivity of passaged viruses to nucleoside analogues at MOI = 0.01 PFU/cell. Cells were treated with the indicated concentrations of 5-FU (C), RBV (D), AZC (E), or CMeA (F) for 30 min prior to infection, supernatants were harvested at 24 hpi, and titers were determined by plaque assay. Data represent changes in titer relative to untreated control results and are plotted as means and standard errors of results from n = 6 (two triplicate experiments). For panels C to F, the statistical significance of changes in the titer of MHV-ExoN(-) P3 relative to MHV-ExoN(-) P250 was determined using the Mann-Whitney test (*, P
    Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 635164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Bio-Rad pvdf membranes
    Immunoblots of cell cycle-controlling proteins in LCSP-treated CRC cells. Cells were treated with increasing concentrations of LCSP as indicated and then incubated at 37°C for 24 h. Cell protein lysates from Colo320DM (a) and SW480 (b) cells were separated by <t>SDS-PAGE,</t> transferred to <t>PVDF</t> membranes, and immunoblotted to show cyclin D1, cyclin A, and cyclin B1 levels, and the beta-actin level as a loading control.
    Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 25649 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher streptomycin
    Immunoblots of cell cycle-controlling proteins in LCSP-treated CRC cells. Cells were treated with increasing concentrations of LCSP as indicated and then incubated at 37°C for 24 h. Cell protein lysates from Colo320DM (a) and SW480 (b) cells were separated by <t>SDS-PAGE,</t> transferred to <t>PVDF</t> membranes, and immunoblotted to show cyclin D1, cyclin A, and cyclin B1 levels, and the beta-actin level as a loading control.
    Streptomycin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 108183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore laminin
    Culture of human induced neural stem cells in 3D in vitro bioengineered brain tissue constructs infused with decellularized brain ECM-collagen I hydrogel. The process starts with decellularization of porcine brains and silk scaffold preparation. Scaffolds are punched into 6 mm diameter constructs with a 2 mm diameter central hole. <t>Laminin-coated</t> scaffolds are seeded with dissociated human induced neural stem cells (hiNSCs). Decellularized ECM is mixed with collagen I and added to the scaffolds seeded with cells. The cell-seeded silk-scaffolds are flooded with media after complete gelation of ECM-collagen I. The center of the construct shows a dense axonal network, surrounded by the neuronal cell bodies and astrocytes. “Adapted with permission from Sood, Disha, et al . “Fetal brain extracellular matrix boosts neuronal network formation in 3D bioengineered model of cortical brain tissue.” ACS biomaterials science engineering 2.1 (2015): 131–140. Copyright 2016 American Chemical Society.”
    Laminin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tropifluor pvdf membrane pore size
    Culture of human induced neural stem cells in 3D in vitro bioengineered brain tissue constructs infused with decellularized brain ECM-collagen I hydrogel. The process starts with decellularization of porcine brains and silk scaffold preparation. Scaffolds are punched into 6 mm diameter constructs with a 2 mm diameter central hole. <t>Laminin-coated</t> scaffolds are seeded with dissociated human induced neural stem cells (hiNSCs). Decellularized ECM is mixed with collagen I and added to the scaffolds seeded with cells. The cell-seeded silk-scaffolds are flooded with media after complete gelation of ECM-collagen I. The center of the construct shows a dense axonal network, surrounded by the neuronal cell bodies and astrocytes. “Adapted with permission from Sood, Disha, et al . “Fetal brain extracellular matrix boosts neuronal network formation in 3D bioengineered model of cortical brain tissue.” ACS biomaterials science engineering 2.1 (2015): 131–140. Copyright 2016 American Chemical Society.”
    Tropifluor Pvdf Membrane Pore Size, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore porcine skin
    Culture of human induced neural stem cells in 3D in vitro bioengineered brain tissue constructs infused with decellularized brain ECM-collagen I hydrogel. The process starts with decellularization of porcine brains and silk scaffold preparation. Scaffolds are punched into 6 mm diameter constructs with a 2 mm diameter central hole. <t>Laminin-coated</t> scaffolds are seeded with dissociated human induced neural stem cells (hiNSCs). Decellularized ECM is mixed with collagen I and added to the scaffolds seeded with cells. The cell-seeded silk-scaffolds are flooded with media after complete gelation of ECM-collagen I. The center of the construct shows a dense axonal network, surrounded by the neuronal cell bodies and astrocytes. “Adapted with permission from Sood, Disha, et al . “Fetal brain extracellular matrix boosts neuronal network formation in 3D bioengineered model of cortical brain tissue.” ACS biomaterials science engineering 2.1 (2015): 131–140. Copyright 2016 American Chemical Society.”
    Porcine Skin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 979 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dulbecco s modified eagle s medium
    Culture of human induced neural stem cells in 3D in vitro bioengineered brain tissue constructs infused with decellularized brain ECM-collagen I hydrogel. The process starts with decellularization of porcine brains and silk scaffold preparation. Scaffolds are punched into 6 mm diameter constructs with a 2 mm diameter central hole. <t>Laminin-coated</t> scaffolds are seeded with dissociated human induced neural stem cells (hiNSCs). Decellularized ECM is mixed with collagen I and added to the scaffolds seeded with cells. The cell-seeded silk-scaffolds are flooded with media after complete gelation of ECM-collagen I. The center of the construct shows a dense axonal network, surrounded by the neuronal cell bodies and astrocytes. “Adapted with permission from Sood, Disha, et al . “Fetal brain extracellular matrix boosts neuronal network formation in 3D bioengineered model of cortical brain tissue.” ACS biomaterials science engineering 2.1 (2015): 131–140. Copyright 2016 American Chemical Society.”
    Dulbecco S Modified Eagle S Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 111459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pvdf membrane
    FoxM1 expression is variable in melanoma cell lines. Whole cell protein lysates were prepared from cultured melanoma cells as listed. Proteins (30 µg) were separated by <t>SDS-PAGE</t> prior to western transfer to <t>PVDF</t> membrane. Membranes were probed with antibodies targeting FOXM1 and phospho-AKT (Ser473). HRP-tagged secondary antibodies were detected with enhanced chemiluminescence. Antibodies targeting GAPDH or β-actin were used to determine equal loading. MELRMU8 (SK-MEL-28#8) is a subclone of original ATCC strain. FOXM1, Forkhead box M1.
    Pvdf Membrane, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare fbs
    Adipogenic, chondrogenic and osteogenic differentiations of WJ-MSCs cultured in <t>DMEM-LG</t> supplemented with 10 % <t>FBS</t> and ESCM under hypoxic condition at P4 ( a ) and P7 ( b ). Adipogenic differentiation was evidenced by the formation of lipid vacuoles
    Fbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 28650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy mini kit
    Adipogenic, chondrogenic and osteogenic differentiations of WJ-MSCs cultured in <t>DMEM-LG</t> supplemented with 10 % <t>FBS</t> and ESCM under hypoxic condition at P4 ( a ) and P7 ( b ). Adipogenic differentiation was evidenced by the formation of lipid vacuoles
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 343576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore l glutamine
    Adipogenic, chondrogenic and osteogenic differentiations of WJ-MSCs cultured in <t>DMEM-LG</t> supplemented with 10 % <t>FBS</t> and ESCM under hypoxic condition at P4 ( a ) and P7 ( b ). Adipogenic differentiation was evidenced by the formation of lipid vacuoles
    L Glutamine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 52942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Localization of bovine 20α-HSD protein expression in the CL throughout the estrous cycle by immunohistochemistry. Representative immunohistochemical analyses using 20α-HSD antiserum (1:1000) and goat anti-rabbit secondary antibodies (1:500). Preimmune serum (1:1000) was used for primary antiserum as the negative control. Immunohistochemistry was performed by a Vectastain ABC kit. Ovarian sections are shown during the estrous cycle. Preimmune serum was used as the control for the CL stage 7 below the right panel. Black bar=100 μm. Red arrows indicate luteal cells.

    Journal: Reproduction (Cambridge, England)

    Article Title: Molecular characterization of bovine placental and ovarian 20?-hydroxysteroid dehydrogenase

    doi: 10.1530/REP-11-0093

    Figure Lengend Snippet: Localization of bovine 20α-HSD protein expression in the CL throughout the estrous cycle by immunohistochemistry. Representative immunohistochemical analyses using 20α-HSD antiserum (1:1000) and goat anti-rabbit secondary antibodies (1:500). Preimmune serum (1:1000) was used for primary antiserum as the negative control. Immunohistochemistry was performed by a Vectastain ABC kit. Ovarian sections are shown during the estrous cycle. Preimmune serum was used as the control for the CL stage 7 below the right panel. Black bar=100 μm. Red arrows indicate luteal cells.

    Article Snippet: Immunohistochemistry Immunohistochemical staining of CH2, CH3, CL3, CL2, and CL1 samples was performed by the Vectastain ABC kit (Vector Laboratories).

    Techniques: Expressing, Immunohistochemistry, Negative Control

    Localization of CD148 + CD27 + and CD148 − CD27 − B cells in follicles of human spleen. Serial tissue sections of human spleen were incubated with anti-IgD antiserum alone ( blue ; A and D ), anti-IgD antiserum ( blue ) and anti-CD27 mAb ( red ; B and E ), or anti-IgD antiserum ( blue ) and anti-IgM mAb ( red ; C ). The anti-IgD polyclonal antibody was visualized after the addition of alkaline phosphatase–conjugated anti–goat Ig and phosphatase-specific substrate. The anti-CD27 or anti-IgM mAb was visualized by an anti–mouse Ig-specific Vectastain kit. Original magnification of A , B , and C : ×40; of D and E : ×100. The follicular mantle zone ( FM ), marginal zone ( MZ ), germinal center ( G or GC ), and T cell zones ( T ) are indicated.

    Journal: The Journal of Experimental Medicine

    Article Title: Identification of Functional Human Splenic Memory B Cells by Expression of CD148 and CD27

    doi:

    Figure Lengend Snippet: Localization of CD148 + CD27 + and CD148 − CD27 − B cells in follicles of human spleen. Serial tissue sections of human spleen were incubated with anti-IgD antiserum alone ( blue ; A and D ), anti-IgD antiserum ( blue ) and anti-CD27 mAb ( red ; B and E ), or anti-IgD antiserum ( blue ) and anti-IgM mAb ( red ; C ). The anti-IgD polyclonal antibody was visualized after the addition of alkaline phosphatase–conjugated anti–goat Ig and phosphatase-specific substrate. The anti-CD27 or anti-IgM mAb was visualized by an anti–mouse Ig-specific Vectastain kit. Original magnification of A , B , and C : ×40; of D and E : ×100. The follicular mantle zone ( FM ), marginal zone ( MZ ), germinal center ( G or GC ), and T cell zones ( T ) are indicated.

    Article Snippet: Bound mouse mAb was visualized using a mouse Ig–specific Vectastain kit (Vector Laboratories, Inc., Burlingame, CA), according to the manufacturer's instructions.

    Techniques: Incubation

    Hypoacetylation of H4K16 is a hallmark of CRC tissues. ( A ) Normal, primary and metastatic CRC tissues were subjected to immunohistochemical analyses using H4K16ac antibodies (Abcam, catalogue number ab109463) and a Vectastain Elite kit was used for secondary antibody and Avidin-Biotin Complex (ABC) conjugation. Staining was considered positive when a brown nuclear reaction was observed. A representative image from each condition is shown. Control; IHC was conducted without the incubation of primary antibodies. ( B ). IHC staining was quantified using ImageJ software (version 1.49, NIH, USA) and presented as average ± SEM. A paired Student's t -test was used to determine the statistical significance between normal, primary and metastatic carcinoma. Asterisks denote P

    Journal: Nucleic Acids Research

    Article Title: Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan

    doi: 10.1093/nar/gkw1026

    Figure Lengend Snippet: Hypoacetylation of H4K16 is a hallmark of CRC tissues. ( A ) Normal, primary and metastatic CRC tissues were subjected to immunohistochemical analyses using H4K16ac antibodies (Abcam, catalogue number ab109463) and a Vectastain Elite kit was used for secondary antibody and Avidin-Biotin Complex (ABC) conjugation. Staining was considered positive when a brown nuclear reaction was observed. A representative image from each condition is shown. Control; IHC was conducted without the incubation of primary antibodies. ( B ). IHC staining was quantified using ImageJ software (version 1.49, NIH, USA) and presented as average ± SEM. A paired Student's t -test was used to determine the statistical significance between normal, primary and metastatic carcinoma. Asterisks denote P

    Article Snippet: A Vectastain Elite kit was used for secondary antibody and Avidin–Biotin Complex (ABC) at RT in accordance with the manufacturer's instructions (Vector laboratories, Burlingame, USA).

    Techniques: Immunohistochemistry, Avidin-Biotin Assay, Conjugation Assay, Staining, Incubation, Software

    Inhibition of CVB3 replication by PDTC is independent of its antioxidant activity. (A) HeLa cells were sham infected with PBS or infected with CVB3 (MOI = 10) in the presence of various inhibitors. Redox-sensitive fluorescence probe CM-H 2 DCFDA

    Journal:

    Article Title: Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway

    doi: 10.1128/JVI.79.13.8014-8023.2005

    Figure Lengend Snippet: Inhibition of CVB3 replication by PDTC is independent of its antioxidant activity. (A) HeLa cells were sham infected with PBS or infected with CVB3 (MOI = 10) in the presence of various inhibitors. Redox-sensitive fluorescence probe CM-H 2 DCFDA

    Article Snippet: To determine the influences of CVB3 replication on intracellular ROS level, HeLa cells were infected with CVB3 for various periods of time, and a redox-sensitive fluorescence probe, CM-H2 DCFDA, was added to the medium 1 h prior to taking photographs.

    Techniques: Inhibition, Antioxidant Activity Assay, Infection, Fluorescence

    Reactive oxygen species generation is increased during CVB3 replication. (A) HeLa cells were sham infected or infected with CVB3 (MOI = 10) for the indicated time. Redox-sensitive fluorescence probe CM-H 2 DCFDA (15 μM) was added to the

    Journal:

    Article Title: Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway

    doi: 10.1128/JVI.79.13.8014-8023.2005

    Figure Lengend Snippet: Reactive oxygen species generation is increased during CVB3 replication. (A) HeLa cells were sham infected or infected with CVB3 (MOI = 10) for the indicated time. Redox-sensitive fluorescence probe CM-H 2 DCFDA (15 μM) was added to the

    Article Snippet: To determine the influences of CVB3 replication on intracellular ROS level, HeLa cells were infected with CVB3 for various periods of time, and a redox-sensitive fluorescence probe, CM-H2 DCFDA, was added to the medium 1 h prior to taking photographs.

    Techniques: Infection, Fluorescence

    PDTC decreases CVB3 viral RNA expression, viral protein synthesis, and viral progeny release of infected cells. (A) HeLa cells were sham-infected with PBS or infected with CVB3 (MOI = 10) in the presence or absence of 100 μM PDTC in the

    Journal:

    Article Title: Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway

    doi: 10.1128/JVI.79.13.8014-8023.2005

    Figure Lengend Snippet: PDTC decreases CVB3 viral RNA expression, viral protein synthesis, and viral progeny release of infected cells. (A) HeLa cells were sham-infected with PBS or infected with CVB3 (MOI = 10) in the presence or absence of 100 μM PDTC in the

    Article Snippet: To determine the influences of CVB3 replication on intracellular ROS level, HeLa cells were infected with CVB3 for various periods of time, and a redox-sensitive fluorescence probe, CM-H2 DCFDA, was added to the medium 1 h prior to taking photographs.

    Techniques: RNA Expression, Infection

    PDTC promotes cell viability of CVB3-infected cells. HeLa cells were infected with CVB3 (MOI = 10) as described in the legend to Fig. in the presence or absence of various concentrations of PDTC. Cell viability was determined at

    Journal:

    Article Title: Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway

    doi: 10.1128/JVI.79.13.8014-8023.2005

    Figure Lengend Snippet: PDTC promotes cell viability of CVB3-infected cells. HeLa cells were infected with CVB3 (MOI = 10) as described in the legend to Fig. in the presence or absence of various concentrations of PDTC. Cell viability was determined at

    Article Snippet: To determine the influences of CVB3 replication on intracellular ROS level, HeLa cells were infected with CVB3 for various periods of time, and a redox-sensitive fluorescence probe, CM-H2 DCFDA, was added to the medium 1 h prior to taking photographs.

    Techniques: Infection

    Antiviral activity of PDTC depends on serum concentration. (A) HeLa cells were sham infected with PBS or infected with CVB3 (MOI = 10) in serum-free DMEM. One hour postinfection, 100 μM PDTC and various concentrations of NCS were added

    Journal:

    Article Title: Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway

    doi: 10.1128/JVI.79.13.8014-8023.2005

    Figure Lengend Snippet: Antiviral activity of PDTC depends on serum concentration. (A) HeLa cells were sham infected with PBS or infected with CVB3 (MOI = 10) in serum-free DMEM. One hour postinfection, 100 μM PDTC and various concentrations of NCS were added

    Article Snippet: To determine the influences of CVB3 replication on intracellular ROS level, HeLa cells were infected with CVB3 for various periods of time, and a redox-sensitive fluorescence probe, CM-H2 DCFDA, was added to the medium 1 h prior to taking photographs.

    Techniques: Activity Assay, Concentration Assay, Infection

    Copper and zinc contribute to the antiviral activity of PDTC. HeLa cells were infected with CVB3 (MOI = 10) in the presence of increased concentrations of CuSO 4 (A) or ZnSO 4 (B) in the presence or absence of 50 μM PDTC as indicated. Seven

    Journal:

    Article Title: Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway

    doi: 10.1128/JVI.79.13.8014-8023.2005

    Figure Lengend Snippet: Copper and zinc contribute to the antiviral activity of PDTC. HeLa cells were infected with CVB3 (MOI = 10) in the presence of increased concentrations of CuSO 4 (A) or ZnSO 4 (B) in the presence or absence of 50 μM PDTC as indicated. Seven

    Article Snippet: To determine the influences of CVB3 replication on intracellular ROS level, HeLa cells were infected with CVB3 for various periods of time, and a redox-sensitive fluorescence probe, CM-H2 DCFDA, was added to the medium 1 h prior to taking photographs.

    Techniques: Activity Assay, Infection

    PDTC inhibits proteasome-mediated protein degradation. (A) HeLa cells were sham infected with PBS or infected with CVB3 (MOI = 10). PDTC (50 μM or 100 μM), NCS (10%), ZnSO 4 (10 μM), or CuSO 4 (10 μM) was added to

    Journal:

    Article Title: Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway

    doi: 10.1128/JVI.79.13.8014-8023.2005

    Figure Lengend Snippet: PDTC inhibits proteasome-mediated protein degradation. (A) HeLa cells were sham infected with PBS or infected with CVB3 (MOI = 10). PDTC (50 μM or 100 μM), NCS (10%), ZnSO 4 (10 μM), or CuSO 4 (10 μM) was added to

    Article Snippet: To determine the influences of CVB3 replication on intracellular ROS level, HeLa cells were infected with CVB3 for various periods of time, and a redox-sensitive fluorescence probe, CM-H2 DCFDA, was added to the medium 1 h prior to taking photographs.

    Techniques: Infection

    PNx induces increased nuclei numbers in α 1 +/− mice. Left ventricle sections from each experimental group were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue) and cardiac troponin I (cTnI, red) as described in materials

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Reduction of Na/K-ATPase affects cardiac remodeling and increases c-kit cell abundance in partial nephrectomized mice

    doi: 10.1152/ajpheart.00102.2014

    Figure Lengend Snippet: PNx induces increased nuclei numbers in α 1 +/− mice. Left ventricle sections from each experimental group were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue) and cardiac troponin I (cTnI, red) as described in materials

    Article Snippet: To count cardiac cell number, slides were stained for cardiac troponin I (cTnI, a cardiomyocyte specific marker, Cat. No. AB47003, Abcam) and 4′,6-diamidino-2-phenylindole (DAPI, a nuclear marker, Cat. No. D1306, Life Technologies).

    Techniques: Mouse Assay, Staining

    Anti-rotaviral activity of RV6-26. (A) Inhibition of in vitro transcription: EDTA-activated DLP were incubated with 200 nM combining sites of different antibodies and mRNA was synthesized in vitro using selected components of the Riboprobe SP6 system (transcription was mediated by the viral RNA-dependent RNA polymerase not the SP6 DNA-dependent RNA polymerase). First-strand cDNA was synthesized by reverse transcription using a VP6-specific primer. Amplification of cDNA with VP6-specific primers was monitored in a real-time PCR using SYBR Green; the concentrations of RNA estimated from a standard curve constructed using reference RNA extracted from RRV are plotted. (B) Inhibition of rotavirus replication by IgA: polarized monolayers of Caco-2 cells grown on Transwell inserts were treated with polymeric IgA in the basal compartment and inoculated apically with trypsin-activated RRV (MOI = 5) at ambient temperature for 1 h and then cultured for 16 in medium containing trypsin. Amount of rotavirus in the inserts was titrated by inoculating MA104 cells and culturing for 16 h, followed by acetone-fixation and staining with anti-rotavirus polyclonal antibodies conjugated to either Alexa568 or IRDye 800. Detection was done either by scanning on Licor or by counting fluorescent foci.

    Journal: PLoS ONE

    Article Title: Human Rotavirus VP6-Specific Antibodies Mediate Intracellular Neutralization by Binding to a Quaternary Structure in the Transcriptional Pore

    doi: 10.1371/journal.pone.0061101

    Figure Lengend Snippet: Anti-rotaviral activity of RV6-26. (A) Inhibition of in vitro transcription: EDTA-activated DLP were incubated with 200 nM combining sites of different antibodies and mRNA was synthesized in vitro using selected components of the Riboprobe SP6 system (transcription was mediated by the viral RNA-dependent RNA polymerase not the SP6 DNA-dependent RNA polymerase). First-strand cDNA was synthesized by reverse transcription using a VP6-specific primer. Amplification of cDNA with VP6-specific primers was monitored in a real-time PCR using SYBR Green; the concentrations of RNA estimated from a standard curve constructed using reference RNA extracted from RRV are plotted. (B) Inhibition of rotavirus replication by IgA: polarized monolayers of Caco-2 cells grown on Transwell inserts were treated with polymeric IgA in the basal compartment and inoculated apically with trypsin-activated RRV (MOI = 5) at ambient temperature for 1 h and then cultured for 16 in medium containing trypsin. Amount of rotavirus in the inserts was titrated by inoculating MA104 cells and culturing for 16 h, followed by acetone-fixation and staining with anti-rotavirus polyclonal antibodies conjugated to either Alexa568 or IRDye 800. Detection was done either by scanning on Licor or by counting fluorescent foci.

    Article Snippet: Infection medium (IM), used when cells were inoculated with rotavirus and cultured, contained all of the above supplements except serum, and trypsin-EDTA (Invitrogen) was added to a final concentration of 1 µg/mL.

    Techniques: Activity Assay, Inhibition, In Vitro, Incubation, Synthesized, Amplification, Real-time Polymerase Chain Reaction, SYBR Green Assay, Construct, Cell Culture, Staining

    Effects of glyceollin I on colony formation on MCF-7 and BG-1 cells. MCF-7 (A) and BG-1 (B) cells were placed in phenol red-free DMEM supplemented with 5% dextran-coated charcoal-treated FBS for 48 h before plating. MCF-7 or BG-1 cells (1000) were plated

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    Article Title: Glyceollin I, a Novel Antiestrogenic Phytoalexin Isolated from Activated Soy

    doi: 10.1124/jpet.109.160382

    Figure Lengend Snippet: Effects of glyceollin I on colony formation on MCF-7 and BG-1 cells. MCF-7 (A) and BG-1 (B) cells were placed in phenol red-free DMEM supplemented with 5% dextran-coated charcoal-treated FBS for 48 h before plating. MCF-7 or BG-1 cells (1000) were plated

    Article Snippet: Human cancer cell lines derived from breast (MCF-7, ER-positive cells) and ovary (BG-1, ER-positive cells) were cultured in 75-cm2 culture flasks in DMEM (Invitrogen, Carlsbad, CA) supplemented with 10% FBS (Invitrogen), basic minimal minimal essential medium essential (50×; Invitrogen) and essential medium nonessential (100×; Invitrogen) amino acids, sodium pyruvate (100×; Invitrogen), antimycotic-antibiotic [10,000 U/ml penicillin G sodium; 10,000 μg/ml streptomycin sulfate; and 25 μg/ml amphotericin B (Fungizone)], and human recombinant insulin (4 mg/ml; Invitrogen).

    Techniques:

    Glyceollin I Inhibits the ER transcriptional activity in MCF-7 cells. Cells were transfected with pGL2-ERE2x-TK-luciferase plasmid. After 5 h, the transfection medium was removed and replaced with phenol red-free DMEM supplemented with 5% CS-FBS containing

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    Article Title: Glyceollin I, a Novel Antiestrogenic Phytoalexin Isolated from Activated Soy

    doi: 10.1124/jpet.109.160382

    Figure Lengend Snippet: Glyceollin I Inhibits the ER transcriptional activity in MCF-7 cells. Cells were transfected with pGL2-ERE2x-TK-luciferase plasmid. After 5 h, the transfection medium was removed and replaced with phenol red-free DMEM supplemented with 5% CS-FBS containing

    Article Snippet: Human cancer cell lines derived from breast (MCF-7, ER-positive cells) and ovary (BG-1, ER-positive cells) were cultured in 75-cm2 culture flasks in DMEM (Invitrogen, Carlsbad, CA) supplemented with 10% FBS (Invitrogen), basic minimal minimal essential medium essential (50×; Invitrogen) and essential medium nonessential (100×; Invitrogen) amino acids, sodium pyruvate (100×; Invitrogen), antimycotic-antibiotic [10,000 U/ml penicillin G sodium; 10,000 μg/ml streptomycin sulfate; and 25 μg/ml amphotericin B (Fungizone)], and human recombinant insulin (4 mg/ml; Invitrogen).

    Techniques: Activity Assay, Transfection, Luciferase, Plasmid Preparation

    Immunoblot analysis of purified C. difficile toxins A and B. Purified proteins (80 µg each) were subjected to 6% PAGE and transferred onto PVDF membranes. Each membrane was probed using monoclonal primary antibodies specific for toxin A or B. The Pierce ECL Western Blotting Kit was used to detect the bound antibodies. The membrane was exposed to X-ray film (Molecular Technologies, St Louis, MO) and processed using a Konica film processor (Konica Corporation, Tokyo, Japan). Sup, crude culture supernatant; Toxin A, purified toxin A; Toxin B, purified toxin B.

    Journal: PLoS ONE

    Article Title: Bile Salt Inhibition of Host Cell Damage by Clostridium Difficile Toxins

    doi: 10.1371/journal.pone.0079631

    Figure Lengend Snippet: Immunoblot analysis of purified C. difficile toxins A and B. Purified proteins (80 µg each) were subjected to 6% PAGE and transferred onto PVDF membranes. Each membrane was probed using monoclonal primary antibodies specific for toxin A or B. The Pierce ECL Western Blotting Kit was used to detect the bound antibodies. The membrane was exposed to X-ray film (Molecular Technologies, St Louis, MO) and processed using a Konica film processor (Konica Corporation, Tokyo, Japan). Sup, crude culture supernatant; Toxin A, purified toxin A; Toxin B, purified toxin B.

    Article Snippet: Purified C. difficile toxins A and B (80 µg each) were separated on 6% polyacrylamide electrophoresis (PAGE) gels and transferred onto Immun-Blot PVDF membrane (BioRad, Hercules, CA) using a Trans-Blot cell (BioRad) transfer apparatus.

    Techniques: Purification, Polyacrylamide Gel Electrophoresis, Western Blot

    Decahedral AgNPs were highly uniform. (a) High-resolution scanning electron microscopy image of AgNPs. Scale bar: 200 nm. (b) High-resolution transmission electron microscopy image of AgNPs. Scale bar: 100 nm. (c) Dynamic light scattering for characterization of particles’ size and distribution in water and complete medium. (d) Positive linear relationship between different concentrations of AgNPs in DMEM containing 10% (v/v) FBS and 1% (v/v) P–S and the corresponding peak absorbance intensity at 495 nm.

    Journal: Toxicology Research

    Article Title: Cytotoxic and sublethal effects of silver nanoparticles on tendon-derived stem cells – implications for tendon engineering

    doi: 10.1039/c5tx00349k

    Figure Lengend Snippet: Decahedral AgNPs were highly uniform. (a) High-resolution scanning electron microscopy image of AgNPs. Scale bar: 200 nm. (b) High-resolution transmission electron microscopy image of AgNPs. Scale bar: 100 nm. (c) Dynamic light scattering for characterization of particles’ size and distribution in water and complete medium. (d) Positive linear relationship between different concentrations of AgNPs in DMEM containing 10% (v/v) FBS and 1% (v/v) P–S and the corresponding peak absorbance intensity at 495 nm.

    Article Snippet: The released cells were washed in PBS and resuspended in DMEM containing 10% (v/v) FBS and 1% (v/v) P–S (all from Invitrogen Corporation, Carlsbad, USA).

    Techniques: Electron Microscopy, Transmission Assay

    SANS data analysis of MtrCAB in 20 m m HEPES pH 7. 8, 100 m m NaCl, 13% D 2 O, 2.8 m m Fos-choline-12, at 3.5 ( black ) and 9.7 ( gray ) mg/ml. A , Guinier region of MtrCAB with linear fit to data shown suggesting an R g of 54 Å. Residuals of fit are shown above. B , Kratky plot of scaled MtrCAB suggests slight differences between the data at 3.5 and 9.7 mg/ml. C and D , scattering curves with fits to the data giving rise to the P ( r ) distance distribution curves shown in ( D ).

    Journal: The Journal of Biological Chemistry

    Article Title: Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners

    doi: 10.1074/jbc.RA118.001850

    Figure Lengend Snippet: SANS data analysis of MtrCAB in 20 m m HEPES pH 7. 8, 100 m m NaCl, 13% D 2 O, 2.8 m m Fos-choline-12, at 3.5 ( black ) and 9.7 ( gray ) mg/ml. A , Guinier region of MtrCAB with linear fit to data shown suggesting an R g of 54 Å. Residuals of fit are shown above. B , Kratky plot of scaled MtrCAB suggests slight differences between the data at 3.5 and 9.7 mg/ml. C and D , scattering curves with fits to the data giving rise to the P ( r ) distance distribution curves shown in ( D ).

    Article Snippet: MtrCAB and MtrAB were purified as described in supporting data and concentrated to ∼15 mg/ml using a 100,000 molecular weight cut-off spin concentrator (Millipore) and dialyzed overnight in a sealed DURAN bottle containing 20 mm HEPES, pH 7.8, plus 100 mm NaCl plus 13% D2 O plus 2.8 mm Fos–choline-12 using a 50,000 molecular weight cut-off Dispo-Biodialyzer (Sigma-Aldrich).

    Techniques:

    Small angle neutron scattering of MtrAB in 20 m m HEPES pH 7.8, 100 m m NaCl, 13% D 2 O, 2.8 m m Fos-choline-12. A , Guinier region of MtrAB at 3.5 ( black ) and 8.7 ( gray ) mg/ml. Lines are linear fits used to calculate R g . Residuals of fit are shown above experimental curve. B , Kratky plot of scaled and overlaid 3.5 ( black ) and 8.7 ( gray ) mg/ml. C , P ( r ) distance distribution curve of merged MtrAB data sets. D , scattering curve of merged 3.5 and 8.7 mg/ml datasets. The line is a fit of the P ( r ) curve shown in ( C ) to the scattering data. E , molecular envelope generated by DAMFILT, representing the core features of 19 independent models selected from 20 generated models fitted to the experimental data.

    Journal: The Journal of Biological Chemistry

    Article Title: Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners

    doi: 10.1074/jbc.RA118.001850

    Figure Lengend Snippet: Small angle neutron scattering of MtrAB in 20 m m HEPES pH 7.8, 100 m m NaCl, 13% D 2 O, 2.8 m m Fos-choline-12. A , Guinier region of MtrAB at 3.5 ( black ) and 8.7 ( gray ) mg/ml. Lines are linear fits used to calculate R g . Residuals of fit are shown above experimental curve. B , Kratky plot of scaled and overlaid 3.5 ( black ) and 8.7 ( gray ) mg/ml. C , P ( r ) distance distribution curve of merged MtrAB data sets. D , scattering curve of merged 3.5 and 8.7 mg/ml datasets. The line is a fit of the P ( r ) curve shown in ( C ) to the scattering data. E , molecular envelope generated by DAMFILT, representing the core features of 19 independent models selected from 20 generated models fitted to the experimental data.

    Article Snippet: MtrCAB and MtrAB were purified as described in supporting data and concentrated to ∼15 mg/ml using a 100,000 molecular weight cut-off spin concentrator (Millipore) and dialyzed overnight in a sealed DURAN bottle containing 20 mm HEPES, pH 7.8, plus 100 mm NaCl plus 13% D2 O plus 2.8 mm Fos–choline-12 using a 50,000 molecular weight cut-off Dispo-Biodialyzer (Sigma-Aldrich).

    Techniques: Generated

    MHV-ExoN(-) evolved WT-like genomic RNA accumulation and increased resistance to multiple nucleoside analogues over the passage. (A) Cells were infected with the indicated viruses at MOI = 1 PFU/cell, and intracellular RNA was harvested using TRIzol at the indicated times postinfection. MHV genomic RNA was detected using SYBR green and primers directed to nsp10, and values were normalized to intracellular GAPDH. (B) Same data as in panel A normalized to the RNA level for each virus at 4 hpi. Data represent means and standard errors of results for n = 9 (3 triplicate experiments). (C to F) Sensitivity of passaged viruses to nucleoside analogues at MOI = 0.01 PFU/cell. Cells were treated with the indicated concentrations of 5-FU (C), RBV (D), AZC (E), or CMeA (F) for 30 min prior to infection, supernatants were harvested at 24 hpi, and titers were determined by plaque assay. Data represent changes in titer relative to untreated control results and are plotted as means and standard errors of results from n = 6 (two triplicate experiments). For panels C to F, the statistical significance of changes in the titer of MHV-ExoN(-) P3 relative to MHV-ExoN(-) P250 was determined using the Mann-Whitney test (*, P

    Journal: mBio

    Article Title: Proofreading-Deficient Coronaviruses Adapt for Increased Fitness over Long-Term Passage without Reversion of Exoribonuclease-Inactivating Mutations

    doi: 10.1128/mBio.01503-17

    Figure Lengend Snippet: MHV-ExoN(-) evolved WT-like genomic RNA accumulation and increased resistance to multiple nucleoside analogues over the passage. (A) Cells were infected with the indicated viruses at MOI = 1 PFU/cell, and intracellular RNA was harvested using TRIzol at the indicated times postinfection. MHV genomic RNA was detected using SYBR green and primers directed to nsp10, and values were normalized to intracellular GAPDH. (B) Same data as in panel A normalized to the RNA level for each virus at 4 hpi. Data represent means and standard errors of results for n = 9 (3 triplicate experiments). (C to F) Sensitivity of passaged viruses to nucleoside analogues at MOI = 0.01 PFU/cell. Cells were treated with the indicated concentrations of 5-FU (C), RBV (D), AZC (E), or CMeA (F) for 30 min prior to infection, supernatants were harvested at 24 hpi, and titers were determined by plaque assay. Data represent changes in titer relative to untreated control results and are plotted as means and standard errors of results from n = 6 (two triplicate experiments). For panels C to F, the statistical significance of changes in the titer of MHV-ExoN(-) P3 relative to MHV-ExoN(-) P250 was determined using the Mann-Whitney test (*, P

    Article Snippet: Total RNA was harvested for most passages using 1 ml of TRIzol reagent (Ambion) per 25-cm2 flask and stored at −80°C.

    Techniques: Infection, SYBR Green Assay, Plaque Assay, MANN-WHITNEY

    Reduced intestinal antimicrobial peptide expression in NY8.3NOD mice in the absence of MyD88. (A and B) Gene expression for the intestinal antimicrobial peptides. Fragments (∼3 cm) of the small intestines from ileum and large intestine adjacent to the cecum were harvested from 6–7-wk-old sex-matched WT NY8.3NOD and MyD88 −/− NY8.3NOD mice. After intensive washing with sterile PBS, RNA was extracted from the fragments using TRIzol, followed by reverse transcription. qPCR was performed to determine the mRNA levels of antimicrobial peptides, which were normalized to the housekeeping gene GAPDH. (A) Small intestine. (B) Large intestine. Five to eight mice/group from two independent experiments are shown. One-tailed Mann-Whitney test was used for statistical analysis. Data are shown as mean ± SEM. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Microbial antigen mimics activate diabetogenic CD8 T cells in NOD mice

    doi: 10.1084/jem.20160526

    Figure Lengend Snippet: Reduced intestinal antimicrobial peptide expression in NY8.3NOD mice in the absence of MyD88. (A and B) Gene expression for the intestinal antimicrobial peptides. Fragments (∼3 cm) of the small intestines from ileum and large intestine adjacent to the cecum were harvested from 6–7-wk-old sex-matched WT NY8.3NOD and MyD88 −/− NY8.3NOD mice. After intensive washing with sterile PBS, RNA was extracted from the fragments using TRIzol, followed by reverse transcription. qPCR was performed to determine the mRNA levels of antimicrobial peptides, which were normalized to the housekeeping gene GAPDH. (A) Small intestine. (B) Large intestine. Five to eight mice/group from two independent experiments are shown. One-tailed Mann-Whitney test was used for statistical analysis. Data are shown as mean ± SEM. *, P

    Article Snippet: Total RNA was extracted from the gut fragments with TRIzol and reverse transcribed to cDNA using a SuperScript III First-strand synthesis kit with random hexamers (Invitrogen). qPCR was performed with the specific primers for Reg3β, Reg3γ, C-reactive protein–ductin, RELMβ, and DEFCR-rs-10 ( ).

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, One-tailed Test, MANN-WHITNEY

    Immunoblots of cell cycle-controlling proteins in LCSP-treated CRC cells. Cells were treated with increasing concentrations of LCSP as indicated and then incubated at 37°C for 24 h. Cell protein lysates from Colo320DM (a) and SW480 (b) cells were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted to show cyclin D1, cyclin A, and cyclin B1 levels, and the beta-actin level as a loading control.

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Induction of Apoptosis and Cell Cycle Arrest in Human Colorectal Carcinoma by Litchi Seed Extract

    doi: 10.1155/2012/341479

    Figure Lengend Snippet: Immunoblots of cell cycle-controlling proteins in LCSP-treated CRC cells. Cells were treated with increasing concentrations of LCSP as indicated and then incubated at 37°C for 24 h. Cell protein lysates from Colo320DM (a) and SW480 (b) cells were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted to show cyclin D1, cyclin A, and cyclin B1 levels, and the beta-actin level as a loading control.

    Article Snippet: For immunoblot analysis, the cell extract was subjected to 12% SDS-PAGE and the resolved bands were electrotransferred to PVDF membranes using semidry blot apparatus (Bio-Rad) at 3 mA per cm2 of the gel in transfer buffer (25 mM tris, pH 8.3, 192 mM glycine, and 20% methanol) at room temperature for 30 min.

    Techniques: Western Blot, Incubation, SDS Page

    Immunoblots of apoptosis-controlling proteins in LCSP-treated colorectal carcinoma cells. The cell lysates from LCSP-treated SW480 (a) and Colo320DM (b) were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted to show the levels of Bax, Bcl-2, procaspase 3 and cleavage-PARP. The protein levels of Bax and Bcl-2 were quantified using ImageJ software according to the density of each band on the immunoblotting image, normalized to the reference band ( β -actin), and presented as the fold of the untreated control. (c) The data reported are the averages of three independent experiments and are expressed as means ± SD. *Represents a significant difference ( P

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Induction of Apoptosis and Cell Cycle Arrest in Human Colorectal Carcinoma by Litchi Seed Extract

    doi: 10.1155/2012/341479

    Figure Lengend Snippet: Immunoblots of apoptosis-controlling proteins in LCSP-treated colorectal carcinoma cells. The cell lysates from LCSP-treated SW480 (a) and Colo320DM (b) were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted to show the levels of Bax, Bcl-2, procaspase 3 and cleavage-PARP. The protein levels of Bax and Bcl-2 were quantified using ImageJ software according to the density of each band on the immunoblotting image, normalized to the reference band ( β -actin), and presented as the fold of the untreated control. (c) The data reported are the averages of three independent experiments and are expressed as means ± SD. *Represents a significant difference ( P

    Article Snippet: For immunoblot analysis, the cell extract was subjected to 12% SDS-PAGE and the resolved bands were electrotransferred to PVDF membranes using semidry blot apparatus (Bio-Rad) at 3 mA per cm2 of the gel in transfer buffer (25 mM tris, pH 8.3, 192 mM glycine, and 20% methanol) at room temperature for 30 min.

    Techniques: Western Blot, SDS Page, Software

    Culture of human induced neural stem cells in 3D in vitro bioengineered brain tissue constructs infused with decellularized brain ECM-collagen I hydrogel. The process starts with decellularization of porcine brains and silk scaffold preparation. Scaffolds are punched into 6 mm diameter constructs with a 2 mm diameter central hole. Laminin-coated scaffolds are seeded with dissociated human induced neural stem cells (hiNSCs). Decellularized ECM is mixed with collagen I and added to the scaffolds seeded with cells. The cell-seeded silk-scaffolds are flooded with media after complete gelation of ECM-collagen I. The center of the construct shows a dense axonal network, surrounded by the neuronal cell bodies and astrocytes. “Adapted with permission from Sood, Disha, et al . “Fetal brain extracellular matrix boosts neuronal network formation in 3D bioengineered model of cortical brain tissue.” ACS biomaterials science engineering 2.1 (2015): 131–140. Copyright 2016 American Chemical Society.”

    Journal: Scientific Reports

    Article Title: Functional maturation of human neural stem cells in a 3D bioengineered brain model enriched with fetal brain-derived matrix

    doi: 10.1038/s41598-019-54248-1

    Figure Lengend Snippet: Culture of human induced neural stem cells in 3D in vitro bioengineered brain tissue constructs infused with decellularized brain ECM-collagen I hydrogel. The process starts with decellularization of porcine brains and silk scaffold preparation. Scaffolds are punched into 6 mm diameter constructs with a 2 mm diameter central hole. Laminin-coated scaffolds are seeded with dissociated human induced neural stem cells (hiNSCs). Decellularized ECM is mixed with collagen I and added to the scaffolds seeded with cells. The cell-seeded silk-scaffolds are flooded with media after complete gelation of ECM-collagen I. The center of the construct shows a dense axonal network, surrounded by the neuronal cell bodies and astrocytes. “Adapted with permission from Sood, Disha, et al . “Fetal brain extracellular matrix boosts neuronal network formation in 3D bioengineered model of cortical brain tissue.” ACS biomaterials science engineering 2.1 (2015): 131–140. Copyright 2016 American Chemical Society.”

    Article Snippet: Briefly, porous silk 3D scaffolds were coated with 0.25 mg/mL laminin (Sigma-Aldrich) either overnight at 4 °C or for 2 h at 37 °C.

    Techniques: In Vitro, Construct

    FoxM1 expression is variable in melanoma cell lines. Whole cell protein lysates were prepared from cultured melanoma cells as listed. Proteins (30 µg) were separated by SDS-PAGE prior to western transfer to PVDF membrane. Membranes were probed with antibodies targeting FOXM1 and phospho-AKT (Ser473). HRP-tagged secondary antibodies were detected with enhanced chemiluminescence. Antibodies targeting GAPDH or β-actin were used to determine equal loading. MELRMU8 (SK-MEL-28#8) is a subclone of original ATCC strain. FOXM1, Forkhead box M1.

    Journal: Oncology Letters

    Article Title: Effects of FOXM1 inhibition and ionizing radiation on melanoma cells

    doi: 10.3892/ol.2018.9482

    Figure Lengend Snippet: FoxM1 expression is variable in melanoma cell lines. Whole cell protein lysates were prepared from cultured melanoma cells as listed. Proteins (30 µg) were separated by SDS-PAGE prior to western transfer to PVDF membrane. Membranes were probed with antibodies targeting FOXM1 and phospho-AKT (Ser473). HRP-tagged secondary antibodies were detected with enhanced chemiluminescence. Antibodies targeting GAPDH or β-actin were used to determine equal loading. MELRMU8 (SK-MEL-28#8) is a subclone of original ATCC strain. FOXM1, Forkhead box M1.

    Article Snippet: Whole cell protein extracts (30 µg) were resolved by SDS-PAGE, transferred to a PVDF membrane using the iblot transfer system (Thermo Fisher Scientific, Inc.) and probed with primary antibodies, species-specific HRP-conjugated secondary antibodies and detected by enhanced chemiluminescence.

    Techniques: Expressing, Cell Culture, SDS Page, Western Blot

    Adipogenic, chondrogenic and osteogenic differentiations of WJ-MSCs cultured in DMEM-LG supplemented with 10 % FBS and ESCM under hypoxic condition at P4 ( a ) and P7 ( b ). Adipogenic differentiation was evidenced by the formation of lipid vacuoles

    Journal: Cytotechnology

    Article Title: Embryonic stem cells conditioned medium enhances Wharton’s jelly-derived mesenchymal stem cells expansion under hypoxic condition

    doi: 10.1007/s10616-014-9708-1

    Figure Lengend Snippet: Adipogenic, chondrogenic and osteogenic differentiations of WJ-MSCs cultured in DMEM-LG supplemented with 10 % FBS and ESCM under hypoxic condition at P4 ( a ) and P7 ( b ). Adipogenic differentiation was evidenced by the formation of lipid vacuoles

    Article Snippet: An equal volume of Dulbecco’s modified Eagle’s medium with 1.0 g/l glucose (DMEM-LG) (Invitrogen) containing 50 % FBS (HyClone, Logan, UT, USA) was added and centrifuged at 3,200 g , 4 °C for 10 min.

    Techniques: Cell Culture

    Cell proliferation, cell morphology and population doubling time (PDT) of DMEM-MSCs and ESCM-MSCs. a An in vitro expansion of WJ-MSCs by DMEM-LG supplemented with 10 % FBS and ESCM under hypoxic condition. Fold increases of DMEM-MSCs and ESCM-MSCs

    Journal: Cytotechnology

    Article Title: Embryonic stem cells conditioned medium enhances Wharton’s jelly-derived mesenchymal stem cells expansion under hypoxic condition

    doi: 10.1007/s10616-014-9708-1

    Figure Lengend Snippet: Cell proliferation, cell morphology and population doubling time (PDT) of DMEM-MSCs and ESCM-MSCs. a An in vitro expansion of WJ-MSCs by DMEM-LG supplemented with 10 % FBS and ESCM under hypoxic condition. Fold increases of DMEM-MSCs and ESCM-MSCs

    Article Snippet: An equal volume of Dulbecco’s modified Eagle’s medium with 1.0 g/l glucose (DMEM-LG) (Invitrogen) containing 50 % FBS (HyClone, Logan, UT, USA) was added and centrifuged at 3,200 g , 4 °C for 10 min.

    Techniques: In Vitro

    Immunophenotype characteristics of WJ-MSCs grown in DMEM-LG supplemented with 10 % FBS and ESCM under hypoxic condition at P4 ( a , b ) and P7 ( c , d ). Positive staining of each marker was shown as percentage in average for DMEM-MSCs and ESCM-MSCs

    Journal: Cytotechnology

    Article Title: Embryonic stem cells conditioned medium enhances Wharton’s jelly-derived mesenchymal stem cells expansion under hypoxic condition

    doi: 10.1007/s10616-014-9708-1

    Figure Lengend Snippet: Immunophenotype characteristics of WJ-MSCs grown in DMEM-LG supplemented with 10 % FBS and ESCM under hypoxic condition at P4 ( a , b ) and P7 ( c , d ). Positive staining of each marker was shown as percentage in average for DMEM-MSCs and ESCM-MSCs

    Article Snippet: An equal volume of Dulbecco’s modified Eagle’s medium with 1.0 g/l glucose (DMEM-LG) (Invitrogen) containing 50 % FBS (HyClone, Logan, UT, USA) was added and centrifuged at 3,200 g , 4 °C for 10 min.

    Techniques: Staining, Marker

    RT-PCR analysis of stemness gene expressions of WJ-MSCs grown in DMEM-LG supplemented with 10 % FBS ( lane 1 ) and ESCM ( lane 2 ) under hypoxic condition at P4 ( a ) and P7 ( b ). GAPDH mRNA expression was detected as PCR positive control and input template

    Journal: Cytotechnology

    Article Title: Embryonic stem cells conditioned medium enhances Wharton’s jelly-derived mesenchymal stem cells expansion under hypoxic condition

    doi: 10.1007/s10616-014-9708-1

    Figure Lengend Snippet: RT-PCR analysis of stemness gene expressions of WJ-MSCs grown in DMEM-LG supplemented with 10 % FBS ( lane 1 ) and ESCM ( lane 2 ) under hypoxic condition at P4 ( a ) and P7 ( b ). GAPDH mRNA expression was detected as PCR positive control and input template

    Article Snippet: An equal volume of Dulbecco’s modified Eagle’s medium with 1.0 g/l glucose (DMEM-LG) (Invitrogen) containing 50 % FBS (HyClone, Logan, UT, USA) was added and centrifuged at 3,200 g , 4 °C for 10 min.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Polymerase Chain Reaction, Positive Control